NeuropharmacologyCorticosterone and dexamethasone potentiate cytotoxicity associated with oxygen-glucose deprivation in organotypic cerebellar slice cultures
Section snippets
Organotypic cerebellar slice culture preparation
Preparation of cerebellar cultures followed procedures described by Stoppini et al. (1991) with modifications as detailed below. Cerebella from 8-day old male and female Sprague–Dawley rat pups (Harlan, Indianapolis, IN, USA) were aseptically removed and placed into ice-cold dissecting medium (Minimum Essential Medium with 2mM l-glutamine plus 25mM HEPES and 50μM penicillin/streptomycin solutions). Using a McIllwain tissue chopper (Mickle Laboratory Engineering Co. Ltd., Gomshall, UK), each
OGD
Initial studies examined the effects of 90-min exposure to OGD on propidium iodide uptake. Exposure to OGD resulted in significant elevations in uptake of propidium iodide when compared with control cultures at 24-, 48-, and 72-h post-insult [F(1,162)=16.084, P<0.001, post hoc P<0.05; Fig. 1]. Representative images are presented as a part of Fig. 3. In addition, analysis failed to demonstrate a significant main effect for time, though propidium iodide uptake was reduced with each subsequent
Discussion
This report has demonstrated that 90-min exposure to OGD in organotypic cerebellar slice cultures resulted in significant cytotoxicity, at 24-, 48-, and 72-h post-insult. Propidium iodide fluorescence in OGD-treated cultures was lower at 72-h when compared with levels observed 24-h post-insult and varied between experiment. The reason for this is unknown; however, it may be related to microglia activation or exclusion of propidium iodide. Exposure to trolox, a potent antioxidant, completely
References (85)
- et al.
Regulation of ischemic cell death by glucocorticoids and adrenocorticotropic hormone
Neuroscience
(1999) - et al.
Glucocorticoid endangerment of hippocampal neurons is NMDA-receptor dependent
Brain Res
(1990) - et al.
Stress-induced changes in messenger RNA levels of N-methyl-D-aspartate and AMPA receptor subunits in selected regions of the rat hippocampus and hypothalamus
Neuroscience
(1995) - et al.
Neuroprotective effects of progesterone on damage elicited by acute global cerebral ischemia in neurons of the caudate nucleus
Arch Med Res
(2002) Glutamate neurotoxicity and diseases of the nervous system
Neuron
(1988)- et al.
Serum-cortisol reflects severity and mortality in acute stroke
J Neurol Sci
(2004) The rehabilitation of elderly stroke patients
Clin Geriatr Med
(1991)- et al.
Corticosterone impairs hippocampal neuronal calcium regulation-possible mediating mechanisms
Brain Res
(1993) - et al.
Progesterone receptor isoforms expression in the prepuberal and adult male rat brain
Brain Res Bull
(2001) - et al.
Progesterone is neuroprotective after transient middle cerebral artery occlusion in male rats
Brain Res
(1996)
Ischemia-induced death of astrocytes and neurons in primary culturepitfalls in quantifying neuronal cell death
Brain Res Dev Brain Res
Dehydroepiandrosterone sulphate prevents oxygen-glucose deprivation-induced injury in cerebellar granule cell culture
Neuroscience
Philanthotoxin inhibits Ca2+ currents in rat hippocampal CA1 neurons
Eur J Pharmacol
Down-regulation of the hypothalamo-pituitary-adrenal axis reduces brain damage and number of seizures following hypoxia/ischaemia in rats
Brain Res
A single social stress-experience alters glutamate receptor-binding in rat hippocampal CA3 area
Neurosci Lett
Glucocorticoids accelerate ATP loss following metabolic insults in cultured hippocampal neurons
Brain Res
Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus) 2. Short-term effect
Comp Biochem Physiol B Biochem Mol Biol
Mifepristone protects CA1 hippocampal neurons following traumatic brain injury in rat
Neuroscience
Cytotoxicity of tributyltin in rat hippocampal slice cultures
Neurosci Res
Regulation of ischemic hippocampal damage in the gerbiladrenalectomy alters the rate of CA1 cell disappearance
Exp Neurol
(-)-Nicotine ameliorates corticosterone’s potentiation of N-methyl-d-aspartate receptor-mediated cornu ammonis 1 toxicity
Neuroscience
Serum cortisol and outcome of ischemic brain infarction
J Neurol Sci
The glucose paradox of cerebral ischemiaevidence for corticosterone involvement
Brain Res
Protective effect of a new anti-oxidant on the rat brain exposed to ischemia-reperfusion injuryinhibition of free radical formation and lipid peroxidation
Free Radic Biol Med
Energy metabolism, stress hormones and neural recovery from cerebral ischemia/hypoxia
Neurochem Int
Ligand and subfield specificity of corticoid-induced neuronal loss in the rat hippocampal formation
Neuroscience
A simple method for organotypic cultures of nervous tissue
J Neurosci Methods
Alteration of neuronal calcium homeostasis and excitotoxic vulnerability by chronic depolarization
Brain Res
Chronic corticosterone treatment induces parallel changes in N-methyl-D-aspartate receptor subunit messenger RNA levels and antagonist binding sites in the hippocampus
Neuroscience
Brain injury and repair mechanismsthe potential for pharmacologic therapy in closed-head trauma
Ann Emerg Med
Glucocorticoids exacerbate insult-induced declines in metabolism in selectively vulnerable hippocampal cell fields
Brain Res
Isoflurane preconditioning reduces Purkinje cell death in an in vitro model of rat cerebellar ischemia
Neuroscience
Detection of free radicals during brain ischemia and reperfusion by spin trapping and microdialysis
Neurosci Lett
Dexamethasone aggravates ischemia-induced neuronal damage by facilitating the onset of anoxic depolarization and the increase in the intracellular Ca2+ concentration in gerbil hippocampus
J Cereb Blood Flow Metab
Hypoglycemic brain injurymetabolic and structural findings in rat cerebellar cortex during profound insulin-induced hypoglycemia and in the recovery period following glucose administration
J Cereb Blood Flow Metab
Immunochemical detection of the mineralocorticoid receptor in rat brain
Neuroendocrinology
The spectrum of cerebellar infarctions
Neurology
Molecular mechanisms of glutamate-dependent neurodegeneration in ischemia and traumatic brain injury
Cell Mol Life Sci
Fertility control in womenresults with RU 486 by the end of 1985
J Steroid Biochem
Contragestion and other clinical applications of RU 486, an antiprogesterone at the receptor
Science
Protection against oxidative stress-induced neuronal cell deatha novel role for RU486
Eur J Neurosci
Dexamethasone target sites in the central nervous system and their potential relevance to mental illness
Cell Mol Neurobiol
Cited by (0)
- 1
Present address: Medical University of South Carolina, Center for Drug and Alcohol Programs IOP4N, 67 President Street, Charleston, SC 29425, USA.