Quantitative assessment of developing afferent patterns in the cat inferior colliculus revealed with calbindin immunohistochemistry and tract tracing methods
Section snippets
Experimental procedures
Brains for this study were obtained from five adult cats and 10 kittens (1, 4, 8, 11, 15, 28, and 42 postnatal-days-old (P)). All animal care and experimental procedures were carried out so as to minimize the number of animals used and any pain or suffering and were in compliance with the Guide for the Care and Use of Laboratory Animals in facilities accredited by the American Association for the Accreditation of Laboratory Animal Care (AAALAC). Experimental protocols were approved by the
CB-immunostained afferent compartment in CNIC in neonatal kitten
At birth (P1), about a week before hearing onset in kittens (Shipley et al 1980, Walsh and McGee 1987), a dense afferent plexus in the IC was immunostained for CB (Fig. 1). There was a decreasing gradient in distribution of CB-immunostained cells from the superficial layers to the deep layers of the DCIC that continued into the central region of IC (Zettel et al 1991, Kelley et al 1992, Friauf 1994, Forster and Illing 2000). In contrast, the dense plexus of heavily CB-immunostained axonal
Discussion
The results show that CB immunostaining in CNIC includes an afferent plexus distributed in distinct bands parallel to fibrodendritic layers. CB bands are present at birth in kittens as well as in adult cats. Compared with CB bands in kittens, adult CB bands increase markedly in thickness, but are only slightly longer. However, these changes in CB band thickness and length differ significantly relative to overall IC growth. On the one hand, as IC size increases, relative band thickness and
Acknowledgments
Supported by DC 04412, DC 00813, NS 36916 and NS 35008.
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