Sensory systemProperties of external plexiform layer interneurons in mouse olfactory bulb slices
Section snippets
Recording methods
Juvenile and mature 22–34 day old C57BL/6J mice from Jackson Laboratories (Bar Harbor, ME, USA) and transgenic mice reared at the University of Maryland, Baltimore, MD, USA, were used. The transgenic mice expressed enhanced green fluorescent protein (GFP) under control of the regulatory region of the 65 kDa mouse glutamic acid decarboxylase (GAD) 65 gene (Erdélyi et al 2002, Galarreta et al 2004). Horizontal slices (400μm thick) were obtained using a vibratome (Series 1000, Ted Pella Inc.,
Morphology
Voltage- and current-clamp recordings were obtained from 20 histologically verified interneurons: 12 in slices from C57BL/6J mice and eight in slices from GAD65-GFP mice. All 20 of the interneurons were distinguished by their varicose processes (Fig. 1A, B, and D). The cell bodies were round-to-oval in shape, with average major and minor dimensions of 12.6±0.6μm (mean±S.E.M.) and 9.1±0.4μm, respectively. Both the GL and mitral cell layer (MCL) borders were visible in sections containing 19 of
Discussion
This study provides new information about the poorly understood interneurons found in the EPL of the main olfactory bulb. Biocytin staining shows that the mouse interneurons resemble the VG and parvalbumin-IR multipolar interneurons described in the hamster and rat using other staining methods (Schneider and Macrides 1978, Kosaka et al 1994). Like many of the hamster and rat interneurons, the mouse interneurons do not appear to bear axons. The axons of EPL interneurons might only be
Acknowledgments
We thank Jason Aungst, Phil Heyward, Sergei Karnup, Stephanie Parrish-Aungst, and Adam Puche for technical assistance, Frank Margolis for the GAD65-GFP mice, Faith Scipio for genotyping, and Michael Shipley and the Department of Anatomy and Neurobiology for sabbatical leave support for K. A. Hamilton at the University of Maryland, Baltimore. Support contributed by NIH grants DC00347, DC03112, DC03195, DC06356, DC36940, and the Biomedical Research Foundation of Northwest Louisiana.
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Present address: Department of Anatomy, Howard University College of Medicine, Washington, DC 20059, USA (T. Heinbockel); Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA (M. Ennis, A. Hayar).