TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons
Section snippets
Preparation of mouse embryos
Pregnant C57BL/6 mice (Nihon SLC, Hamamatsu, Japan) were used in the present study. These mice were kept under a 12-h light/dark cycle with food and water ad libitum. At all times, the experiments were carried out under the control of the Animal Research Control Committee in accordance with The Guidelines for Animal Experiments of Wakayama Medical University, Japanese Government Notification on Feeding and Safekeeping of Animals (No. 6), and the National Institute of Health Guide for the Care
Developmental expression of TRPV2 in DRGs, spinal cord, and skin
TRPV2-positive cells were first observed in the mouse DRGs at E11.5 (Fig. 1A). At this stage, all TRPV2-positive cells expressed NeuN, indicating that these cells were postmitotic neurons (Mullen et al., 1992; data not shown). TRPV2 expression in the DRG neurons was observed up to P56 (Figs. 1B, C, 5). There were no significant changes in the percentage of TRPV2-positive neurons in total neuronal population from 13.4±0.6% (121/858 cells) at E13.5 to 18.7±2.3% (229/1230 cells) at P56 (Fig. 3).
Discussion
TRPV2 is expressed in a subpopulation of medium- to large-sized neurons in adult DRGs (Caterina et al., 1999; Ma, 2001; Lewinter et al., 2004). Large-sized neurons are generated between E9 and E10 in mice, and most of which begin to express TrkC between E10 and E11 (Farinas et al., 1998; Ma et al., 1999). In the present study, we found that DRGs began to express TRPV2 between E9.5 and E11.5. In addition, TRPV2 expression was mainly observed in TrkC-positive DRG neurons at E11.5 and E13.5. NT-3
Acknowledgments
We thank Dr. Makoto Tominaga (Section of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences) for the generous gift of the TRPV2 antiserum. We are also grateful to Dr. Keiichiro Okamoto (Department of Physiology, Wakayama Medical University) for critical advices of the statistical analysis. This work was supported by Grant-in-Aid for Scientific Research (C) from The Ministry of Education, Culture, Sports, Science and Technology (15500264) and
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