TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons

doi:10.1016/j.neuroscience.2004.09.021

Neuroscience

Volume 130, Issue 1, 2005, Pages 223-228

https://doi.org/10.1016/j.neuroscience.2004.09.021 Get rights and content

Abstract

TRPV2, a member of transient receptor potential ion channels, responds to high-threshold noxious heat, but neither to capsaicin nor to proton. Although TRPV2 is expressed in medium- to large-sized dorsal root ganglion (DRG) neurons with myelinated fibers in adult rodents, little is known about the neurotrophin dependence of TRPV2-positive neurons in the developing and adult DRGs of mice. In the present study, using immunohistochemistry, we found that TRPV2 was first expressed in DRG neurons at embryonic day (E) 11.5, when neither TRPV1 nor TRPM8 was detected yet. Double-immunofluorescence staining revealed that tyrosine kinase receptor C (TrkC) was expressed in most of TRPV2-positive DRG neurons at E11.5 and E13.5. In addition, the percentage of TRPV2-positive neurons in the total DRG neurons at E13.5 reached the same as that of adulthood. In adult DRGs, TrkC and Ret were expressed in 68% and 25% of TRPV2-positive neurons, respectively. These results suggest that TRPV2 is expressed predominantly in the NT-3-dependent subpopulation of DRG neurons throughout development and in adult mice.

Section snippets

Preparation of mouse embryos

Pregnant C57BL/6 mice (Nihon SLC, Hamamatsu, Japan) were used in the present study. These mice were kept under a 12-h light/dark cycle with food and water ad libitum. At all times, the experiments were carried out under the control of the Animal Research Control Committee in accordance with The Guidelines for Animal Experiments of Wakayama Medical University, Japanese Government Notification on Feeding and Safekeeping of Animals (No. 6), and the National Institute of Health Guide for the Care

Developmental expression of TRPV2 in DRGs, spinal cord, and skin

TRPV2-positive cells were first observed in the mouse DRGs at E11.5 (Fig. 1A). At this stage, all TRPV2-positive cells expressed NeuN, indicating that these cells were postmitotic neurons (Mullen et al., 1992; data not shown). TRPV2 expression in the DRG neurons was observed up to P56 (Figs. 1B, C, 5). There were no significant changes in the percentage of TRPV2-positive neurons in total neuronal population from 13.4±0.6% (121/858 cells) at E13.5 to 18.7±2.3% (229/1230 cells) at P56 (Fig. 3).

Discussion

TRPV2 is expressed in a subpopulation of medium- to large-sized neurons in adult DRGs (Caterina et al., 1999; Ma, 2001; Lewinter et al., 2004). Large-sized neurons are generated between E9 and E10 in mice, and most of which begin to express TrkC between E10 and E11 (Farinas et al., 1998; Ma et al., 1999). In the present study, we found that DRGs began to express TRPV2 between E9.5 and E11.5. In addition, TRPV2 expression was mainly observed in TrkC-positive DRG neurons at E11.5 and E13.5. NT-3

Acknowledgments

We thank Dr. Makoto Tominaga (Section of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences) for the generous gift of the TRPV2 antiserum. We are also grateful to Dr. Keiichiro Okamoto (Department of Physiology, Wakayama Medical University) for critical advices of the statistical analysis. This work was supported by Grant-in-Aid for Scientific Research (C) from The Ministry of Education, Culture, Sports, Science and Technology (15500264) and

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TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons

Abstract

Section snippets

Preparation of mouse embryos

Developmental expression of TRPV2 in DRGs, spinal cord, and skin

Discussion

Acknowledgments

Neuron

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Neuroscience

Neuron

Neuron

Cell

Neuron

Pain

Neuron

The GDNF family: signaling, biological functions and therapeutic value

Nat Rev Neurosci

Postnatal changes in the expression of the trkA high-affinity NGF receptor in primary sensory neurons

Eur J Neurosci

The capsaicin receptor: a heat-activated ion channel in the pain pathway

Nature

A capsaicin-receptor homologue with a high threshold for noxious heat

Nature

Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor

Nature