Central projections of sensory innervation of the rat superior sagittal sinus
Section snippets
Experimental procedures
Sixteen adult male Sprague–Dawley rats weighing 280–400 g were used in this study. The animals received food and water ad libitum and were kept in a 12-h light/dark cycle at a constant environmental room temperature. Experimental procedures were approved in advance by the Animal Care and Use Committee of Malmö/Lund.All experiments conformed in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC). All efforts were made to minimize the number of animals used
Labeling of the SSS and ganglia
Examination of the application sites showed that in most cases intense CTb or WGA–HRP labeling was limited to the adventitia of the SSS and the immediately adjacent dura. In one of the WGA–HRP cases, the tracer spread slightly into neighboring dura. As described below, the transganglionic labeling pattern in this case was somewhat different from that seen in the other cases.
In both the CTb and WGA–HRP cases, numerous labeled cell bodies were observed bilaterally in the TGs and C2 DRGs (Fig. 2A1,
Technical considerations
In the present study we examined the central projections of the SSS sensory innervation in rat using transganglionic tract tracing techniques. CTb or choleragenoid-conjugated horseradish peroxidase (B-HRP) label both large and small diameter myelinated A-fibers, which mainly originate from low threshold mechanoreceptors and terminate in the deep part (laminae III–V) of the spinal dorsal horn. WGA–HRP preferentially labels unmyelinated C-fibers which terminate in the superficial layers (laminae
Acknowledgments
This project was supported by the Swedish Research Council (Project Nos. 5958 and 14276).
References (39)
- et al.
Origin and distribution of cerebral vascular innervation from superior cervical, trigeminal and spinal ganglia investigated with retrograde and anterograde WGA–HRP tracing in the rat
Neuroscience
(1986) - et al.
Activation of trigeminal brain-stem nociceptive neurons by dural artery stimulation
Pain
(1986) - et al.
Glutamatergic transmission in the trigeminal nucleus assessed with local blood flow
Brain Res
(2000) - et al.
Stimulation of an intracranial trigeminally-innervated structure selectively increases cerebral blood flow
Brain Res
(1997) - et al.
Stimulation of the trigeminal ganglion increases flow in the extracerebral but not the cerebral circulation of the monkey
Brain Res
(1986) - et al.
Degenerative changes in primary trigeminal axons and in neurons in nucleus caudalis following tooth pulp extirpations in the cat
Brain Res
(1977) Projection patterns of primary sensory neurons studied by transganglionic methods: somatotopy and target-related organization
Brain Res Bull
(1993)- et al.
Comparative effects of stimulation of the trigeminal ganglion and the superior sagittal sinus on cerebral blood flow and evoked potentials in the cat
Brain Res
(1988) - et al.
Differential termination of large-diameter and small-diameter primary afferent fibers in the spinal dorsal gray matter as indicated by labeling with horseradish peroxidase
Neurosci Lett
(1977) - et al.
Central projections of sensory innervation of the rat superficial temporal artery
Brain Res
(2003)
Transganglionic transport of wheat germ agglutinin-HRP and choleragenoid-HRP in rat trigeminal primary sensory neurons
Brain Res
A comparison between wheat germ agglutinin-and choleragenoid-horseradish peroxidase as anterogradely transported markers in central branches of primary sensory neurones in the rat with some observations in the cat
Neuroscience
Response of brainstem trigeminal neurons to electrical stimulation of the dura
Brain Res
Neuronal pathways to the rat middle meningeal artery revealed by retrograde tracing and immunocytochemistry
J Auton Nerv Syst
Brain stem terminations of the trigeminal and upper spinal ganglia innervation of the cerebrovascular system: WGA–HRP transganglionic study
J Cereb Blood Flow Metab
Enrichment of glutamate-like immunoreactivity in primary afferent terminals throughout the spinal cord dorsal horn
Eur J Neurosci
Spinal and trigeminal mechanisms of nociception
Annu Rev Neurosci
aspects on the pathophysiology of migraine and cluster headache
Pharmacol Toxicol
Retrograde tracing of nerve fibers to the rat middle cerebral artery with true blue: colocalization with different peptides
J Cereb Blood Flow Metab
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