Ultrastructural analysis of the central terminals of primary sensory neurones labelled by transganglionic transport of bandeiraea simplicifolia i-isolectin B4
Section snippets
Animals
Three adult Wistar strain rats with body weights ranging from 240 to 430 g were used in this study. All animal work was carried out in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals and the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes and with the approval of the University Research Ethics Committee. All efforts were made to minimise the number of animals used in this experiment and their suffering.
Dorsal root ganglia
Light microscopic examination of vibratome sections of the L4 dorsal root ganglia in all three animals revealed a dense reaction product associated with a large number of neurones indicating that lectin transport had been successful. Although no detailed morphometric analysis was performed, it was clear that lectin transport was restricted to a subpopulation of small diameter neurones (Fig. 1A).
Examination at higher magnification, and with the aid of an oil-immersion objective, revealed that
Discussion
This study constitutes the first detailed analysis of the ultrastructural appearance of the central terminals of primary sensory neurones labelled by transganglionic transport of BSI-B4. The results raise a number of interesting issues regarding the mode of transport of the lectin and the features of the neurones that it labels.
Acknowledgements
This study was supported by an Australian Postgraduate Award from the Commonwealth Department of Education, Science and Training to M.B.G. and a Queensland University of Technology Research Encouragement Award to M.B.P.
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