Neuron
Volume 76, Issue 2, 18 October 2012, Pages 297-308
Journal home page for Neuron

NeuroResource
Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice

https://doi.org/10.1016/j.neuron.2012.07.011Get rights and content
Under an Elsevier user license
open archive

Summary

The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks.

Highlights

Thy1-GCaMP2.2c and Thy1-GCaMP3 transgenic lines were generated ► Spontaneous and evoked calcium transients can be detected in acute brain slices ► Calcium transients can be detected in dendrites and spines in vivo ► Calcium transients can be detected in neuronal populations in vivo

Cited by (0)

10

These authors contributed equally to this work