Elsevier

NeuroImage

Volume 183, December 2018, Pages 897-906
NeuroImage

The frequency of alpha oscillations: Task-dependent modulation and its functional significance

https://doi.org/10.1016/j.neuroimage.2018.08.063Get rights and content

Highlights

  • Alpha frequency (AF) is modulated in a task-dependent fashion.

  • Higher prestimulus AF predicted slower reaction time and weaker stimulus response.

  • Task-modulation of AF and alpha power (AP) are independent.

  • BOLD in visual cortex are negatively coupled with AF but not AP.

  • AF along with AP provides more comprehensive indexing of sensory gating.

Abstract

Power (amplitude) and frequency are two important characteristics of EEG alpha oscillations (8–12 Hz). There is an extensive literature showing that alpha power can be modulated in a goal-oriented manner to either enhance or suppress sensory information processing. Only a few studies to date have examined the task-dependent modulation of alpha frequency. Instead, alpha frequency is often viewed as a trait variable, and used to characterize individual differences in cognitive functioning. We performed two experiments to examine the task-dependent modulation of alpha frequency and its functional significance. In the first experiment, high-density EEG was recorded from 21 participants performing a Sternberg working memory task. The results showed that: (1) during memory encoding, alpha frequency decreased with increasing memory load, whereas during memory retention and retrieval, alpha frequency increased with increasing memory load, (2) higher alpha frequency prior to the onset of probe was associated with longer reaction time, and (3) higher alpha frequency prior to the onset of cue or probe was associated with weaker early cue-evoked or probe-evoked neural responses. In the second experiment, simultaneous EEG-fMRI was recorded from 59 participants during resting state. An EEG-informed fMRI analysis revealed that the spontaneous fluctuations of alpha frequency, but not alpha power, were inversely associated with BOLD activity in the visual cortex. Taken together, these findings suggest that alpha frequency is task-dependent, may serve as an indicator of cortical excitability, and along with alpha power, provides more comprehensive indexing of sensory gating.

Introduction

EEG alpha rhythm (8–12 Hz) is prominent over occipital-parietal cortices in humans during restful wakefulness. Like any oscillatory activity, alpha can be characterized by its amplitude (power) and frequency. Since its initial discovery by Berger (1929), extensive evidence has accumulated to suggest that alpha oscillations are involved in cognitive processing, including attention and working memory (Fink et al., 2005; Jensen et al., 2002; Jensen and Tesche, 2002; Klimesch et al., 1997). In particular, alpha power is considered a reliable indicator of cortical excitability (Jensen and Mazaheri, 2010; Lange et al., 2013) and can be modulated by higher-order brain areas to functionally inhibit or facilitate sensory information processing according to behavioral goals (Liu et al., 2014; Mulholland, 1968; Rajagovindan and Ding, 2011; Wang et al., 2016).

Alpha frequency, in contrast, has been viewed mainly as a trait variable. Individual differences in alpha frequency have been linked to differences in cognitive capabilities (Anokhin and Vogel, 1996; Grandy et al., 2013). An existing theory postulates that alpha frequency is a manifestation of an internal brain clock controlling the speed of information processing (Klimesch et al., 1996); the faster the internal clock (higher individual alpha frequency), the faster the information and cognitive processing (e.g., in memory retrieval and cognitive control). In addition, alpha frequency is known to change systematically over the lifespan (Bernhard and Skoglund, 1939), and is subject to the impact of neurological disorders (Bonanni et al., 2008). Perceptually, individual differences in alpha peak frequency has been shown to predict the temporal windows of the double-flash illusion, suggesting that the peak alpha frequency is the “fingerprint” that drives cross-modal impact on visual perception (Cecere et al., 2015). Similarly, the phasic differences of alpha rhythm has been shown to impact visual perception of rapid sequential stimuli (Minami and Amano, 2017; Valera et al., 1981).

To what extent alpha frequency can be modulated in a task-dependent manner? If such modulation occurs, what is the associated functional significance? These questions have yet to be fully addressed. Two earlier studies by Osaka and by Earle reported inconclusive findings. Osaka showed that alpha frequency increased with task difficulty (Osaka, 1984), but Earle reported that while in some tasks alpha frequency increased when task difficulty increased, in other tasks, the opposite was observed (Earle, 1988). Haegens et al., using an n-back working memory task, found that alpha frequency increased with task difficulty, i.e., higher working memory load was accompanied by higher alpha frequency (Haegens et al., 2014). A more recent study reported that alpha frequency changes according to the types of visual perception such as temporal stimulus integration and temporal stimulus segregation (Wutz et al., 2018). Despite these advances, many questions remain; in particular, the functional and behavioral relevance of alpha frequency modulation by task conditions remains to be further elucidated. Some of the tasks used in previous studies have temporally overlapping cognitive processes. For example, in the n-back task used by Haegens et al. (2014), cognitive processes such as sensory encoding, memory retention and memory retrieval are difficult to separate temporally, and each process may have a differential effect on alpha frequency (Jensen et al., 2002).

Alpha frequency modulation may also be considered in the context of how alpha interacts with other neural processes, such as theta oscillations (Sauseng et al., 2005; Scheeringa et al., 2009), beta oscillations (Yuan et al., 2010), and gamma oscillations (Voytek et al., 2010). In particular, in light of the proposal that gamma cycles are embedded within alpha (Osipova et al., 2008; Roux and Uhlhaas, 2014), alpha frequency modulation may become a means to flexibly increase and decrease the number of gamma cycles accommodated within a cycle of alpha to either facilitate or inhibit sensory processing.

In this study, we considered task-dependent modulation of alpha frequency, its functional significance, and the underlying neural correlates by conducting two experiments. In the first experiment, high-density EEG (128 channels) was recorded from 21 subjects performing a Sternberg working memory task. In this task, memory related cognitive processes such as encoding, retention and retrieval are well separated in time, making it well-suited for analyzing modulations of alpha frequency by distinct cognitive processes. Alpha frequency was estimated during different stages of the task and compared across memory loads. Functional significance was further assessed by correlating the modulations of alpha frequency with behavioral performance and other neural variables such as event-related brain responses. In the second experiment, we examined the neural sources underlying the modulation of scalp level alpha variables, namely, alpha power and alpha frequency. Simultaneous EEG-fMRI was recorded from a cohort of 59 subjects at rest. Utilizing the naturally occurring fluctuations of alpha frequency and alpha power, we examined the possible neural underpinnings of alpha frequency modulation and alpha power modulation by correlating them with the simultaneously recorded fMRI BOLD (Blood Oxygen Level Dependence) fluctuations from different regions of the visual cortex as well as the entire brain.

Section snippets

Procedure and paradigm

The experimental protocol was approved by the Institutional Review Board of the University of Florida (UF IRB). Twenty-one healthy individuals (age: 20 to 34; 3 women) with normal or corrected-to-normal vision gave written informed consent and participated in the study. On each trial of the working memory task, a digit set (cue set) of 1, 3 or 5 distinct numerical digits (0–9) was displayed for 1s (encoding). This was followed by a 3s period during which the subjects held the cue set in working

Behavioral results

Behavioral measures were analyzed using a mixed-effects linear model with performance metrics as the dependent variable, memory load (1, 3 and 5) as the independent variable, and subjects as the random effects. As shown in Fig. 1C and D, reaction time was significantly slower for higher memory load (p < 0.001), whereas accuracy was significantly lower for higher memory load (p < 0.001), in agreement with previous studies of the same paradigm (Jensen and Lisman, 1998; Sternberg, 1969).

Alpha power and frequency modulation by working memory load

Power

Main findings

We performed two experiments to examine the task-dependent modulation of alpha frequency and its functional significance as well as its neural substrate. In the first experiment, the analysis of EEG recorded from healthy subjects performing a Sternberg working memory task shows that: (1) alpha power decreased with memory load during encoding, increased with memory load during retention, and had no systematic relationship with memory load during retrieval, (2) alpha frequency decreased with

Disclosure of interest

The authors report no conflicts of interest.

Acknowledgment

This work was supported by US National Institutes of Health grant MH112206 and US National Science Foundation grant BCS1439188.

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