Analysis of the conserved neurotrophic factor MANF in the Drosophila adult brain
Section snippets
DmMANF is expressed in glial processes
It has been shown that during embryonic stages, DmMANF expression is confined to some glial subpopulations, such as glia surrounding dopaminergic neurons, in longitudinal and in channel glia (Palgi et al., 2009). Here we used immunohistochemistry to identify the cell populations where DmMANF is expressed. We found that in the adult brain, DmMANF is expressed in all glial subtypes, as categorized by Awasaki et al. (2008) and Doherty et al. (2009) (Table 1), thus having a considerably broader
Fly strains
Flies were kept and grown under standard conditions. w1118 flies were regarded as wild type. For the statistical analysis (Fig. 5, Fig. 6), Canton-S flies were used as wild type. We used the following stocks from Bloomington Drosophila Stock Centre (BDSC): UAS-nGFP (4775), UAS-mCD8::GFP (5137), hsFLP (8862), Kr/CyO; D/Tb Sb (7199), FRT82B Gal80 (5135) and UAS-DmMANFRNAi [from Vienna Drosophila RNAi Centre (VDRC) v12835]. The following stocks are the glial subtype drivers obtained from the
Acknowledgements
Most of the antibodies were provided by the Development Studies Hybridoma Bank, and the fly strains were from the Bloomington Drosophila Stock Centre, the Vienna Drosophila RNAi Centre and the Drosophila Genomics Resource Centre. The authors would like to thank Iris Salecker for providing the lama-Gal4 fly stock and Arja Ikävalko for her assistance with crosses and stock maintenance. This work was supported in parts by the Academy of Finland, Marie Curie Early Stage Training, the Finnish
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