Cell Reports
Volume 25, Issue 10, 4 December 2018, Pages 2689-2703.e3
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Article
Single-Cell RNA-Seq of Mouse Olfactory Bulb Reveals Cellular Heterogeneity and Activity-Dependent Molecular Census of Adult-Born Neurons

https://doi.org/10.1016/j.celrep.2018.11.034Get rights and content
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Highlights

  • Single-cell sequencing reveals cellular heterogeneity in the mouse olfactory bulb

  • Differential gene expression uncovers selective markers for cell types

  • Pseudotemporal ordering of adult-born neurons reveals developmentally governed genes

  • Olfactory experience changes the cellular composition of olfactory bulb circuits

Summary

Cellular heterogeneity within the mammalian brain poses a challenge toward understanding its complex functions. Within the olfactory bulb, odor information is processed by subtypes of inhibitory interneurons whose heterogeneity and functionality are influenced by ongoing adult neurogenesis. To investigate this cellular heterogeneity and better understand adult-born neuron development, we utilized single-cell RNA sequencing and computational modeling to reveal diverse and transcriptionally distinct neuronal and nonneuronal cell types. We also analyzed molecular changes during adult-born interneuron maturation and uncovered developmental programs within their gene expression profiles. Finally, we identified that distinct neuronal subtypes are differentially affected by sensory experience. Together, these data provide a transcriptome-based foundation for investigating subtype-specific neuronal function in the olfactory bulb (OB), charting the molecular profiles that arise during the maturation and integration of adult-born neurons and how they dynamically change in an activity-dependent manner.

Keywords

single-cell RNA-seq
adult neurogenesis
olfactory bulb
circuit integration
olfaction
lineage
development
interneuron
transcriptome

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These authors contributed equally

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