Vesicular glutamate transporters in the lateral geniculate nucleus: expression of VGLUT2 by retinal terminals

doi:10.1016/j.brainres.2003.10.032

Brain Research

Volume 996, Issue 2, 23 January 2004, Pages 251-254

https://doi.org/10.1016/j.brainres.2003.10.032 Get rights and content

Abstract

We used immunohistochemistry to localize vesicular glutamate transporters VGLUT1 and VGLUT2 in the rat lateral geniculate nucleus. The lateral geniculate nucleus is intensely immunoreactive for both transporters. Monocular eye removal abolished staining for VGLUT2 in a pattern corresponding to the distribution of terminals from the missing eye, without affecting distribution of VGLUT1 immunoreactivity. These data indicate retinal ganglion cells are the source of VGLUT2-containing synapses in the lateral geniculate nucleus.

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Acknowledgements

Supported by NIH grant NS41428 (P.W.L.); E.K. was supported by NSF REU DBI-0097404. Dr. Susan Erickson and Dr. Deda Gillespie provided helpful comments.

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The slides were washed in PBS followed by blocking and permeabilization for 1 hour in blocking buffer (PBS, 0.5% Triton X-100, 5.5% donkey serum, 5.5% goat serum, pH ∼ 7.4). The slides were then incubated in primary antibodies diluted in blocking buffer (1:250 guinea pig-anti-vGlut2, Millipore AB255; 1:500 rabbit-anti-Iba1, Wako 019-19741) overnight – vGlut2 is a selective marker for retinogeniculate terminals in the dLGN (Fujiyama et al., 2003; Land et al., 2004; Yoshida et al., 2009; Koch et al., 2011; Seabrook et al., 2013), while Iba1 was used to label microglia. Following incubation with primary antibodies, the slices were washed in PBS, blocked for 1 hour and incubated with secondary antibodies diluted in blocking buffer (1:200, goat-anti-guinea pig IgG, Alexa Fluor 488, Invitrogen A11073; 1:200 donkey-anti-rabbit IgG, Alexa Fluor 568, Invitrogen A10042), washed and coverslipped with Vectashield Hardset mounting medium and stored at 4 °C until imaged.
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Short communicationVesicular glutamate transporters in the lateral geniculate nucleus: expression of VGLUT2 by retinal terminals

Abstract

Section snippets

Acknowledgements

J. Biol. Chem.

Brain Res.

Neuron

Biochimie

Mol. Brain Res.

Brain Res.

Brain Res.

Curr. Opin. Neurobiol.

Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter

J. Neurochem.

The localization of the brain-specific inorganic phosphate transporter suggests a specific presynaptic role in glutamatergic transmission

J. Neurosci.

The ventral lateral geniculate nucleus of the albino rat morphological and histochemical observations

J. Hirnforsch.

The identification of vesicular glutamate transporter 3 suggests novel modes of signaling by glutamate

Proc. Natl. Acad. Sci. USA

Immunocytochemical localization of candidates for vesicular glutamate transporters in the rat cerebral cortex

J. Comp. Neurol.

Short communication
Vesicular glutamate transporters in the lateral geniculate nucleus: expression of VGLUT2 by retinal terminals