Priority CommunicationDifferences in Stress-Induced Changes in Extinction and Prefrontal Plasticity in Postweanling and Adult Animals
Section snippets
Methods and Materials
Adult (~60 days old) and postweanling (24–27 days old) male Sprague Dawley rats from the local animal colony at the Haifa University were used (for details, see Supplement 1).
Surgery and electrophysiological recordings were previously reported in our studies (39, 40) (Supplement 1).
Stress procedure was detailed elsewhere (39, 40) (Supplement 1). Corticosterone assessment is detailed in Supplement 1.
Detailed description of the procedure of fear conditioning and extinction was previously detailed
Effects of Exposure to Stress on Extinction in Adult and Postweanling Rats
Adult animals (adults, 60 days old) and postweanling pups (PW, 24–27 days old) were trained to associate a tone with an electrical footshock on the conditioning day. Twenty-four hours after conditioning, the animals were exposed to three tones for fear memory retrieval and immediately thereafter were either exposed to the elevated platform stressor (EP; adults-EP; n = 8; PW-EP; n = 7) or placed back in their home cage (adults-control; n = 8; PW-control; n = 8).
Two-way analysis of variance
Discussion
Using behavior, electrophysiology, and pharmacology, the major finding of this study is that following stress, the response profile of postweanling pups differs from that of the adult animal. However, it should be noted that baseline differences between the two age groups also exist, pointing to developmental differences that will be discussed in the next sections.
Acknowledgments and Disclosures
This project was supported by an Israel Science Foundation grant to MM (663/13). We thank Hamutal Rosengarten and Nissrin Lahoud for valuable help. The authors report no biomedical financial interests or potential conflicts of interest.
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