Biochemical and Biophysical Research Communications
A new putative cyclic nucleotide-gated channel gene, cng-3, is critical for thermotolerance in Caenorhabditis elegans☆
Section snippets
Materials and methods
Caenorhabditis elegans strains and maintenance. Four C. elegans strains were utilized in this study. Bristol N2 and tax-4(p768) strains were obtained from the Caenorhabditis Genetics Center (CGC) at the University of Minnesota, USA. The cng-3(jh113) strain was isolated by reverse genetics method in our laboratory and tax-4(p678); cng-3(jh113) strain was made by a cross between tax-4(p678) and cng-3(jh113) strains. Worm breeding and handling were conducted as described [16].
cDNA cloning and
Characterization of a putative gene encoding a cyclic nucleotide-gated channel in C. elegans
We have identified a new gene, F38E11.12, bearing high homology to the family of cyclic nucleotide-gated channels (Fig. 1A). Complementary DNA (cDNA) encoded by this gene was isolated and named as the cng-3. The cng-3 cDNA contains an open reading-frame of 2241 bp and is predicted to encode a protein of 626 amino acids (Fig. 1B). Hydrophobicity analysis of CNG-3 protein revealed six hydrophobic peaks that could correspond to six membrane-spanning regions (S1–S6). The CNG-3 protein shares 16%
Acknowledgments
We thank A. Coulson for cosmids, the CGC for strains, and K. Cunningham and A. Fire for expression vectors and a cDNA library. We are especially grateful to K. Kim for identification of expression patterns of cng-3; B.-J. Park for technical assistance and helpful advice. This work was supported by the grants from the Korean Ministry of Science and Technology through Institute of Science and Technology for Sustainability at GIST and from Korean Systems Biology Research M1-0309-00-006 and
References (22)
Visual excitation and recovery
J. Biol. Chem.
(1991)- et al.
The odorant-sensitive adenylate cyclase of olfactory receptor cells. Differential stimulation by distinct classes of odorants
J. Biol. Chem.
(1986) - et al.
Rod and cone photoreceptor cells express distinct genes for cGMP-gated channels
Neuron
(1993) - et al.
A putative cyclic nucleotide-gated channel is required for sensory development and function in C. elegans
Neuron
(1996) - et al.
Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C. elegans
Neuron
(1996) - et al.
Functional reconstitution of a heteromeric cyclic nucleotide-gated channel of Caenorhabditis elegans in cultured cells
Brain Res.
(1999) - et al.
DNA transformation
Methods Cell. Biol.
(1995) - et al.
Odorant-selective genes and neurons mediate olfaction in C. elegans
Cell
(1993) - et al.
Induction by cyclic GMP of cationic conductance in plasma membrane of retinal rod outer segment
Nature
(1985) - et al.
Odorant-sensitive adenylate cyclase may mediate olfactory reception
Nature
(1985)
A cyclic nucleotide-gated conductance in olfactory receptor cilia
Nature
Cited by (28)
Photo-and thermo-regulation by photonic crystals for extended longevity of C. elegans
2023, Mechanisms of Ageing and DevelopmentAlteration in expressions of ion channels in Caenorhabditis elegans exposed to polystyrene nanoparticles
2021, ChemosphereCitation Excerpt :Probability level of 0.01 (∗∗) was considered to be statistically significant. Previous studies have raised some genes encoding ion channels required for the control of stress response (Cho et al., 2004; Sanders et al., 2005; LeBoeuf et al., 2007; Park et al., 2009; Sesti et al., 2009; Dwyer and Aamodt, 2013; Wan et al., 2013; Lee et al., 2016; Liang et al., 2017; Scott et al., 2017; Goodman and Sengupta, 2019). After the exposure, PS-NPs (1–1000 μg/L) did not significantly affect expressions of mec-4, mec-2, osm-9, trp-1, tax-2, slo-1, osm-12, unc-103, cup-4, and exc-1 in wild-type worms (Fig. 1C).
A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride Inhibition of an rGC
2016, CellCitation Excerpt :Furthermore, the specific localization of KCC-3 to glial membranes around AFD strongly suggests that these ions function in the vicinity of the AFD NRE. AFD neuronal activity requires a cyclic nucleotide-gated (CNG) channel composed of the TAX-2 β subunit and the TAX-4 or CNG-3 α subunits (Cho et al., 2004; Coburn and Bargmann, 1996; Komatsu et al., 1996). CNG channels allow cations (Na+, K+, and Ca+) to flow across membranes down their electrochemical gradients.
Environmental alkalinity sensing mediated by the transmembrane guanylyl cyclase GCY-14 in C. elegans
2013, Current BiologyCitation Excerpt :To find genes involved in alkalinity sensing, we analyzed a series of mutants defective in NaCl chemotaxis (NaCl is mainly sensed by ASE) (Figure 1B). In addition to dyf-3 and che-1 described above, tax-2 and tax-4 mutants of cyclic nucleotide gated (CNG) channel subunits [16, 17] were also completely defective in chemotaxis to alkaline pH. In contrast, a mutant defective in cng-3, which encodes another CNG channel α subunit expressed in ASE [18], did not show a significant defect in alkaline pH chemotaxis. When tax-2 or tax-4 mutants were stimulated by a pH up-step from 6.8 to 10.0, intracellular Ca2+ levels in ASEL did not change (Figures 1F and S1S).
Toxicology at Environmentally Relevant Concentrations in Caenorhabditis elegans
2022, Toxicology at Environmentally Relevant Concentrations in Caenorhabditis elegansMolecular Strategies for Intensity-Dependent Olfactory Processing in Caenorhabditis elegans
2021, Frontiers in Molecular Neuroscience
- ☆
Abbreviations: CNG channel, cyclic nucleotide-gated channel; GFP, green fluorescent protein; PCR, polymerase chain reaction; CNBD, cyclic nucleotide-binding domain.