Elsevier

Behavioural Brain Research

Volume 281, 15 March 2015, Pages 69-77
Behavioural Brain Research

Research report
TRPC3 channels critically regulate hippocampal excitability and contextual fear memory

https://doi.org/10.1016/j.bbr.2014.12.018Get rights and content
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open access

Highlights

  • Targeted proteomics identify set of proteins correlated with contextual fear memory.

  • Whole-genome transcript profiling highlights Trpc3 as putative regulator of memory.

  • Mechanistic studies demonstrate TRPC3 regulates hippocampal neuronal excitability.

  • Functional studies show TRPC3 regulates contextual fear memory in vivo.

Abstract

Memory formation requires de novo protein synthesis, and memory disorders may result from misregulated synthesis of critical proteins that remain largely unidentified. Plasma membrane ion channels and receptors are likely candidates given their role in regulating neuron excitability, a candidate memory mechanism. Here we conduct targeted molecular monitoring and quantitation of hippocampal plasma membrane proteins from mice with intact or impaired contextual fear memory to identify putative candidates. Here we report contextual fear memory deficits correspond to increased Trpc3 gene and protein expression, and demonstrate TRPC3 regulates hippocampal neuron excitability associated with memory function. These data provide a mechanistic explanation for enhanced contextual fear memory reported herein following knockdown of TRPC3 in hippocampus. Collectively, TRPC3 modulates memory and may be a feasible target to enhance memory and treat memory disorders.

Keywords

Hippocampus
Memory
Transient receptor potential cation channel
Afterhyperpoloarization
Aging
Proteomics

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