[3h]-nociceptin ligand-binding and nociceptin opioid receptor mrna expression in the human brain
Section snippets
Tissue acquisition and slice preparation
Human brain tissues (n=4) were obtained from male patients at medicolegal autopsy. Patients (age: 56–67 years, mean 61 years) had died from heart failure (n=3) or car accident. None of the patients had a history of neurological or psychiatric disease. With a postmortem interval of mean 19.0 h (6–29.5), brain tissues were removed for diagnostic reasons. Representative tissue blocks were prepared and rapidly frozen on dry ice. Sections (20 μm) of brain tissue used in the present study were cut on
Results
In the present study, the differential expression of NOP1 receptors in the human brain (cortex, basal ganglia, hippocampal area and cerebellum) was investigated by utilizing on-section ligand binding corroborated with mRNA detection on parallel sections of the same brain tissues. In general, [3H]-nociceptin ligand binding and NOP1 receptor mRNA expression were widespread and indicative of a considerable high NOP1 receptor expression in these anatomical regions.
[3H]-nociceptin ligand binding was
Discussion
Although the neuroanatomical distribution of the NOP1 receptor has been well characterized in the rodent CNS, to date no data have been presented regarding the expression of this opioid receptor in the adult human brain. The present study closes this gap by describing the patterns of nociceptin ligand binding and NOP1 receptor mRNA expression in neurologically normal adult human postmortem brain. By corroborating binding sites with corresponding sites of transcript expression, this combination
Acknowledgements
This work was supported by SFB 391 of the Deutsche Forschungsgemeinschaft.
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