Extending the cerebellar Lugaro cell class
Section snippets
Experimental procedures
In accordance with the European Communities Directive 86/609/EEC, the strict minimum of experimental animals were used and all efforts were made to avoid any suffering during the perfusion protocol.
Results
The description we provide here is based on a series of 15 Golgi-stained neurons with an impregnated axonal arborization. These were selected by a thorough scanning of serial parasagittal sections of the vermises of a collection of young rat cerebella aged 12–30 post-natal days. Cells with an axon impregnated beyond the initial segment were only found in eight rats aged 16–24 days. This could be due to the development of a myelin sheath around these axons in older animals, thus leading to a
Discussion
We present here a previously undescribed group of large interneurons located in the granular layer of the rat cerebellar cortex. We propose that these globular cells be considered as a subpopulation of an extended Lugaro cell class, with whom they share a unique pattern of axonal projection, a molecular specification by calretinin and an important afferentation by recurrent collaterals of PC axons.
Acknowledgements
The authors sincerely thank M.E. Marc for expert technical help.
References (35)
- et al.
Immunohistochemical localization of calcium-binding protein in the cerebellum, hippocampal formation and olfactory bulb of the rat
Brain Res.
(1982) - et al.
Granular layer collaterals of the unipolar brush cell axon display rosette-like excrescences: a Golgi study in the rat cerebellar cortex
Neurosci. Lett.
(1994) - et al.
Unipolar brush cell: a potential feedforward excitatory interneuron of the cerebellum
Neuroscience
(2000) - et al.
Morphological and neurochemical differentiation of large granular layer interneurons in the adult rat cerebellum
Neuroscience
(2001) - et al.
Choline acetyltransferase immunoreactivity in the cat cerebellum
Neuroscience
(1991) A subtype of cerebellar Golgi cells may be cholinergic
Brain Res.
(1990)Monodendritic neurons: a cell type in the human cerebellar cortex identified by chromogranin A-like immunoreactivity
Brain Res.
(1990)- et al.
Metabotropic glutamate receptors mGluR2 and mGluR5 are expressed in two non-overlapping populations of Golgi cells in the rat cerebellum
Neuroscience
(1996) - et al.
Colocalization of glycine-like and GABA-like immunoreactivities in Golgi cell terminals in the rat cerebellum: a postembedding light and electron microscopic study
Brain Res.
(1988) Immunoreactivity for calretinin and other calcium-binding proteins in cerebellum
Neuroscience
(1989)
Enkephalin immunoreactivity in Golgi cells and mossy fibers of mammalian, avian, amphibian and teleost cerebellum
Neuroscience
Time of origin and distribution of a new cell type in the rat cerebellar cortex
Exp. Brain Res.
Immunohistochemical localization of calretinin in the rat hindbrain
J. Comp. Neurol.
On three types of large nerve cells in the granular layer of the human cerebellar cortex
Anat. Embryol.
Serotonin-driven long-range inhibitory connections in the cerebellar cortex
J. Neurosci.
Distribution of unipolar brush cells and other calretinin immunoreactive components in the mammalian cerebellar cortex
J. Neurocytol.
IPSC kinetics at identified GABAergic and mixed GABAergic and glycinergic synapses onto cerebellar Golgi cells
J. Neurosci.
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