Elsevier

Neuroscience Letters

Volume 312, Issue 1, 12 October 2001, Pages 17-20
Neuroscience Letters

Imaging postsynaptic activities of teleost thalamic neurons at single cell resolution using a voltage-sensitive dye

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Abstract

Optical recording of neuronal activities using voltage-sensitive dyes (VSDs) is a useful method for simultaneous multi-site recording. However, it has been rather difficult to distinguish optical signals from individual, identified cells. We applied the optical recording technique using a high-speed charge coupled device (CCD) imaging system to a teleost thalamic nucleus, corpus glomerulosum (CG) which has a well-defined histological organization and large postsynaptic dendrites. Patch-like dye (di-4-ANEPPS) signals were observed in the dendritic layer of the CG in response to afferent nerve stimulations. These responses were completely blocked by an α-amino-3-hydroxy-5-methyl-4-isoxazole-proprionate (AMPA) receptor antagonist, did not propagate, and the size of the patches were close to that of a single dendritic tip of the ‘large cell’. Thus, we found that these patch-like VSD signals most likely represent postsynaptic potentials at individual dendritic tips of the large cells.

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Acknowledgements

This work was supported by Grants in Aid from the Ministry of Education, Science, Culture, and Sports of Japan to Y.O. and Research Fellowships of the Japan Society for Promotion of Science for Young Scientists to H.T.

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