Cell
Volume 87, Issue 7, 27 December 1996, Pages 1317-1326
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Article
Subregion- and Cell Type–Restricted Gene Knockout in Mouse Brain

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Abstract

Using the phage P1–derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination–based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

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