Innervation of taste buds in the canine larynx as revealed by immunohistochemistry for the various neurochemical markers

doi:10.1016/S0040-8166(97)80009-2

Tissue and Cell

Volume 29, Issue 3, June 1997, Pages 339-346

https://doi.org/10.1016/S0040-8166(97)80009-2 Get rights and content

Abstract

The distribution and innervation of the canine laryngeal taste buds were observed using immuno-histochemistry with antibodies against protein gene product 9.5 (PGP 9.5) and neurofilament protein (NFP). We also observed the immunohistochemical distribution of serotonin, tyrosine hydroxylase (TH) and various neuropeptides including calcitonin gene-related peptide (CGRP), substance P (SP), vasoactive intestinal peptide (VIP), galanin, methionine enkephalin (ENK) and neuropeptide Y (NPY). The taste buds in the canine larynx were densely distributed in the mucosa at the basal portion of the epiglottis and cuneiform process of the arytenoid cartilage. The taste cells were immunoreactive for PGP 9.5 and serotonin. The nerve fibers with immunoreactivity for PGP 9.5 in the taste buds were observed in the perigemmal region and intra- and subgemmal plexuses, and these were classified into two types based on their diameter. The thick nerve fibers corresponded to the fibers immunoreactive for NFP, while the thin nerve fibers corresponded to the fibers immunoreactive for TH and various neuropeptides. Numerous nerve fibers immunoreactive for SP and CGRP were observed in the perigemmal region, and intra- and subgemmal plexuses. A few galanin- and ENK-immunoreactive nerve fibers were also observed in the taste buds, whereas NPY-immunoreactive nerve fibers were noted beneath them. All peptide-containing fibers except for VIP-immunoreactive nerves were situated in the subgemmal regions.

In conclusion, the multiple innervation to the laryngeal taste buds were documented. Thick nerve fibers are likely to be irritant receptors, while thin varicose nerve fibers seem to regulate taste buds themselves. The laryngeal taste buds may be among the important structures which are sensitive to exogeneous chemical and/or mechanical stimuli, for the protection of the airway and the regulation of the respiratory function.

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Innervation of taste buds in the canine larynx as revealed by immunohistochemistry for the various neurochemical markers

Abstract

Regul. Pept.

Regul. Pept.

Exp. Neurol.

Regul. Pept.

Regul. Pept.

Clin. Chest Med.

Respir. Physiol.

Brain Res.

Water-responsive laryngeal receptors in the dog are not specialized endings

Respir. Physiol.

The response of laryngeal afferent fibres to mechanical and chemical stimuli

J. Physiol.

The chemical neuroanatomy of sympathetic ganglia

Ann. Rev. Neurosci.

Peptide immunohistochemistry demonstrates multiple classes of perigemmal nerve fibers in the circumvallate papilla of the rat

Chem. Senses

The paraneuron

Liquid-sensitive receptors in the developing sheep, cat and monkey

J. Physiol.

Histological study on the nerve distribution in the larynx of cat

Arch. Histol. Jpn.

Distribution of calcitonin gene-related peptide nerve fibers in the canine larynx

Eur. Arch. Otorhinolaryngol.

The cytologic composition of primate laryngeal chemosensory corpuscles

Am. J. Anat.

On the innervation of taste-buds in larynx in dog

Tohoku J. Exp. Med.