Visible light effects on tumoral cells in a culture medium enriched with tryptophan and riboflavin

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Abstract

When NSO/2 myeloid cell line and teratocarcinoma F9 cells were irradiated in Dulbecco's modified Eagle medium enriched with tryptophan and riboflavin, toxic photoproducts for these tumoral cells were generated. The active participation of 1O2 and ·OH was established using specific scavengers and quenchers. A cytotoxic effect was also observed when unirradiated tumoral cells were incubated in a previously irradiated culture medium enriched with tryptophan and riboflavin. When irradiated medium was used alone, enriched only with tryptophan or only with riboflavin, no toxic effect was observed. The relevance of charge transfer processes between triplet riboflavin and tryptophan in the generation of cytotoxic photoproducts is discussed.

References (37)

  • R.B. Misra et al.

    Riboflavin induced phototoxicity to Paramecium

    Indian J. Exp. Biol.

    (1987)
  • R.B. Misra et al.

    Phototoxic effects of riboflavin in Tetrahymena thermophila

    Indian J. Exp. Biol.

    (1990)
  • M.N. Donoso et al.

    Tryptophan—riboflavin photoinduced adduct and hepatic dysfunction in rats

    Nutr. Rep. Int.

    (1988)
  • E. Silva et al.

    Toxic effect of a photoinduced tryptophan—riboflavin adduct on F9-Teratocarcinoma cells and preimplantation mouse embryos

    Int. J. Vit. Nutr. Res.

    (1988)
  • I. Ferrer et al.

    Study of a photoinduced lysozyme—riboflavin bond

    Radiat. Environ. Biophys.

    (1985)
  • M. Salim-Hanna et al.

    Obtention of a photoinduced adduct between a vitamin and an essential amino acid. Binding of riboflavin to tryptophan

    Int. J. Vit. Nutr. Res.

    (1987)
  • J. Rojas et al.

    Photochemical-like behaviour of riboflavin in the dark promoted by enzyme-generated triplet acetone

    Photochem. Photobiol.

    (1988)
  • J.H. Luft

    Improvements in epoxy resin methods

    J. Biophys. Biochem. Cytol.

    (1961)
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