Neuron
Volume 2, Issue 6, June 1989, Pages 1597-1603
Journal home page for Neuron

PC12 cell
L1-mediated axon outgrowth occurs via a homophilic binding mechanism

https://doi.org/10.1016/0896-6273(89)90048-2Get rights and content

Abstract

The molecular mechanism by which the L1 cell adhesion molecule mediates neurite outgrowth has been examined. Purified L1 from mouse and L1 from chick brain were attached to nitrocellulose dishes. Both chick and mouse neurons were able to adhere to purified mouse L1 and chick L1. Both molecules promoted neurite extension from chick and mouse neurons. Addition of Fabs specific for chick L1 to the cultures inhibited chick neurite outgrowth on both mouse L1 and chick L1. These findings suggest that L1-like molecules support neurite outgrowth via a “homophilic” binding mechanism.

References (28)

  • R. Hawkes et al.

    A dot-immunobinding assay for monoclonal and other antibodies

    Anal. Biochem.

    (1982)
  • R.D. Lund et al.

    Uses of a species specific antibody for demonstrating mouse neurons transplanted to rat brains

    Neurosci. Lett.

    (1985)
  • M.G. Mage

    Preparation of Fab fragments from IgGs of different animal species

    Meth. Enzymol.

    (1980)
  • J.L. Bixby et al.

    Identification of the major proteins that promote neuronal process outgrowth on Schwann cells in vitro

    J. Cell Biol.

    (1988)
  • E. Bock et al.

    Demonstration of immunochemical identity between the nerve growth factor-inducible large external (NILE) glycoprotein and the cell adhesion molecule L1

    EMBO J.

    (1985)
  • S. Chang et al.

    Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins

    J. Cell Biol.

    (1987)
  • G.J. Cole et al.

    A heparin-binding domain from NCAM is involved in neural cell-substratum adhesion

    J. Cell Biol.

    (1986)
  • J. Covalt et al.

    Neurite outgrowth on cryostat sections of innervated and denervated skeletal muscle

    J. Cell Biol.

    (1987)
  • J.K. Daniloff et al.

    Differential distribution of cell adhesion molecules during histogenesis of the chick nervous system

    J. Neurosci.

    (1986)
  • S.F. De St. Groth et al.

    Production of monoclonal antibodies: strategies and tactics

    J. Immunol. Meth.

    (1980)
  • D.R. Friedlander et al.

    Nerve growth factor enhances expression of neuron-glia cell adhesion molecule in PC12 cells

    J. Cell Biol.

    (1986)
  • M. Grumet et al.

    Heterotypic binding between neuronal membrane vesicles and glial cells is mediated by a specific cell adhesion molecule

    J. Cell Biol.

    (1984)
  • M. Grumet et al.

    Neuron-glia cell adhesion molecule interacts with neurons and astroglia via different binding mechanisms

    J. Cell Biol.

    (1988)
  • M. Grumet et al.

    Two antigenically related neuronal cell adhesion molecules of different specificities mediate neuron-neuron and neuron-glia adhesion

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    Present address: Center for Neurosciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.

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