Elsevier

Neuroscience

Volume 73, Issue 2, July 1996, Pages 299-315
Neuroscience

Correlated morphological and neurochemical features identify different subsets of vasoactive intestinal polypeptide-immunoreactive interneurons in rat hippocampus

https://doi.org/10.1016/0306-4522(95)00610-9Get rights and content

Abstract

Vasoactive intestinal polypeptide-immunoreactive interneurons have been classified according to their axonal and dendritic patterns and neurochemical features in the hippocampus of the rat. A correlation of these characteristics unravelled three distinct types of vasoactive intestinal polypeptide-containing cells. Intemeurons forming a dense axonal plexus at the border of stratum oriens and alveus always contain the calcium binding protein, calretinin, but lack the neuropeptide cholecystokinin. The axon of another type of vasoactive intestinal polypeptide-positive interneuron surrounds pyramidal cell bodies in a basket-like manner, and co-localizes cholecystokinin but not calretinin. Vasoactive intestinal polypeptide-containing cells projecting to stratum radiatum form two subsets distinguished by dendritic morphology. Those with dendrites restricted to stratum lacunosum-moleculare lack both calretinin and cholecystokinin, whereas the other subtype with dendrites spanning all layers contains calretinin in 40% of the cases and occasionally also cholecystokin. GABA was shown to be present, and the calcium binding proteins calbindin D-28k and parvalbumin absent from all three types of vasoactive intestinal polypeptide-positive interneurons.

The specific dendritic and axonal arbours imply different input and output properties for the three interneuron types. The correlation of these features with the content of neurochemical markers strongly suggests that they are specialized for distinct inhibitory functions in the hippocampal network.

Reference (50)

  • KosakaT. et al.

    GABAergic neurons containing the Ca2+-binding protein parvalbumin in the rat hippocampus and dentate gyrus

    Brain. Res.

    (1987)
  • LorenI. et al.

    Distribution of vasoactive intestinal polypeptide in the rat and mouse brain

    Neuroscience

    (1979)
  • MiettinenR. et al.

    Calretinin is present in non-pyramidal cells of the rat hippocampus—II. Co-existence with other calcium binding proteins and GABA

    Neuroscience

    (1992)
  • MorrisonJ.H. et al.

    Immunohistochemical distribution of pro-somatostatin-related peptides in hippocampus

    Neurosci. Lett.

    (1982)
  • RobertsG.W. et al.

    Distribution of neuropeptides in the limbic system of the rat: the hippocampus

    Neuroscience

    (1984)
  • RogersJ.H.

    Two calcium-binding proteins mark many chick sensory neurons

    Neuroscience

    (1989)
  • SomogyiP. et al.

    A new type of specific interneuron in the monkey hippocampus forming synapses exclusively with the axon initial segments of pyramidal cells

    Brain. Res.

    (1983)
  • SomogyiP. et al.

    Identified axo-axonic cells are immunoreactive for GABA in the hippocampus and visual cortex of the cat

    Brain. Res.

    (1985)
  • SzabatE. et al.

    A new monoclonal antibody against the GABA-protein conjugate shows immunoreactivity in sensory neurons of the rat

    Neuroscience

    (1992)
  • TothK. et al.

    Calbindin D28k-containing non-pyramidal cells in the rat hippocampus: their immunoreactivity for GABA and projection to the medial septum

    Neuroscience

    (1992)
  • Blasco-IbanezJ.M. et al.

    Synaptic input of horizontal interneurons in stratum oriens of the hippocampal CA1 subfield: structural basis of feedback activation

    Eur. J. Neurosci.

    (1995)
  • BraginA. et al.

    Gamma (40–100 Hz) oscillation in the hippocampus of the behaving rat

    J. Neurosci.

    (1995)
  • BuckmasterP.S. et al.

    Somatostatin-immunoreactivity in the hippocampus of mouse, rat, guinea pig, and rabbit

    Hippocampus

    (1994)
  • BuckmasterP.S. et al.

    Interneurons and inhibition in the dentate gyrus of the rat in vivo

    J. Neurosci

    (1995)
  • BuhlE.H. et al.

    Diverse sources of hippocampal unitary inhibitory postsynaptic potentials and the number of synaptic release sites [see comments]

    Nature

    (1994)
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