Distribution of the growth associated protein GAP-43 in the central processes of axotomized primary afferents in the adult rat spinal cord; presence of growth cone-like structures

doi:10.1016/0304-3940(91)90331-M

Neuroscience Letters

Volume 131, Issue 1, 30 September 1991, Pages 37-41

https://doi.org/10.1016/0304-3940(91)90331-M Get rights and content

Abstract

GAP-43-immunolabelled structures were visualized by electron microscopy in the adult rat L₄–L₅ superficial dorsal horn 2 weeks after sciatic nerve transection. The majority of immunolabelled elements were unmyelinated axons, but some synaptic terminals and myelinated axons also labelled. The labelled unmyelinated axons were commonly located in prominent bundles which on serial section analysis could be followed into larger single trunks. These enlargements contain many organelles and give rise to smaller processes, which is compatible with their being growth cones. Sciatic nerve transection may result, therefore, in central regenerative processes which reorganize the neuropil and contribute to the decreased sensibility and pain that follows peripheral nerve section.

References (29)

J. Arvidsson et al.
Cell loss in lumbar dorsal root ganglia and transganglionic degeneration after sciatic nerve resection in the rat
Brain Res.
(1986)
M.A. Bisby
Dependence of GAP-43 (B50, F1) transport on axonal regeneration in rat dorsal root ganglion cells
Brain Res.
(1988)
C.B. McGuire et al.
Light microscopic immunolocalization of the growth and plasticity-associated protein GAP-43 in the developing rat brain
Dev. Brain Res.
(1988)
M.L. Reynolds et al.
GAP-43 expression in developing cutaneous and muscle nerves in the rat hindlimb
Neuroscience
(1991)
C.J. Woolf et al.
GAP-43, a growth-associated protein, appears in dorsal root ganglion cells and in the dorsal horn of the rat spinal cord following peripheral nerve injury
Neuroscience
(1990)
L.I. Benowitz et al.
Anatomical distribution of the growth-associated protein GAP-43/B-50 in the adult rat brain
J. Neurosci.
(1988)
S.R.Y. Cajal
Histologie du Systeme Nerveuse de l'Homme et des Vertebres
(1911)
J.M. Castro-Lopes et al.
Ultrastructural changes of the central scalloped (C₁) primary afferent endings of synaptic glomeruli in the substantia gelatinosa Rolandi of the rat after peripheral neurotomy
J. Neurocytol.
(1990)
B. Csillik et al.
Regenerative synaptogenesis in the mammalian spinal cord dynamics of synaptochemical restoration in the Rolando substance after transganglionic degenerative atrophy
J. Neural Transm.
(1981)
S.A. Fidel et al.
Effects of manipulation of GAP-43 expression on morphology of PC12 cells and cultured hippocampal neurons
Soc. Neurosci. Abstr.
(1990)

T.G.M.F. Gorgels et al.

B50/GAP-43 is localized at the cytoplasmic side of the plasma membrane in developing and adult pyramidal tract

J. Neurosci.

(1989)

P. Hoffman

Expression of GAP-43, a rapidly transported growth-associated protein and class II beta tubulin, a slowly transported cytoskeletal protein, are co-ordinated in regenerating neurons

J. Neurosci.

(1989)

R.D. Jacobson et al.

A protein associated with growth, GAP 43, is widely distributed and developmentally regulated in rat CNS

J. Neurosci.

(1986)

F.L. Katz et al.

Nerve growth cones isolated from fetal rat brain. III. Calcium-dependent protein phosphorylation

J. Neurosci.

(1985)

Cited by (0)

^☆: This work was supported by the MRC, the Wellcome Trust, a Bristol-Myers Squibb Unrestricted Pain Research Grant and NIH Grants NS10161 and NS 11255.

¹: We thank Dr. L.I. Benowitz for the supply of the antibody.

View full text

Distribution of the growth associated protein GAP-43 in the central processes of axotomized primary afferents in the adult rat spinal cord; presence of growth cone-like structures☆

Abstract

Brain Res.

Brain Res.

Dev. Brain Res.

Neuroscience

Neuroscience

Anatomical distribution of the growth-associated protein GAP-43/B-50 in the adult rat brain

J. Neurosci.

Histologie du Systeme Nerveuse de l'Homme et des Vertebres

Ultrastructural changes of the central scalloped (C1) primary afferent endings of synaptic glomeruli in the substantia gelatinosa Rolandi of the rat after peripheral neurotomy

J. Neurocytol.

Regenerative synaptogenesis in the mammalian spinal cord dynamics of synaptochemical restoration in the Rolando substance after transganglionic degenerative atrophy

J. Neural Transm.

Effects of manipulation of GAP-43 expression on morphology of PC12 cells and cultured hippocampal neurons

Soc. Neurosci. Abstr.

B50/GAP-43 is localized at the cytoplasmic side of the plasma membrane in developing and adult pyramidal tract

J. Neurosci.

Expression of GAP-43, a rapidly transported growth-associated protein and class II beta tubulin, a slowly transported cytoskeletal protein, are co-ordinated in regenerating neurons

J. Neurosci.

A protein associated with growth, GAP 43, is widely distributed and developmentally regulated in rat CNS

J. Neurosci.

Nerve growth cones isolated from fetal rat brain. III. Calcium-dependent protein phosphorylation

J. Neurosci.

Ultrastructural changes of the central scalloped (C₁) primary afferent endings of synaptic glomeruli in the substantia gelatinosa Rolandi of the rat after peripheral neurotomy