Pitfalls in the use of brain slices
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Appendix—Brain slice methods
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Cited by (86)
Relationship between artificial cerebrospinal fluid oxygenation, slice depth and tissue performance in submerged brain slice experiments
2020, Neuroscience LettersCitation Excerpt :Thus, factors that affect oxygenation are important to consider, especially in experiments where tissue oxygen levels are not directly recorded or controlled. In submerged slices, tissue oxygenation is known to be highly dependent upon artificial cerebrospinal fluid (aCSF) flow rate [2–4]. A less well studied variable is the depth of slice submersion.
Normobaric hyperoxia (95% O<inf>2</inf>) stimulates CO<inf>2</inf>-sensitive and CO<inf>2</inf>-insensitive neurons in the caudal solitary complex of rat medullary tissue slices maintained in 40% O<inf>2</inf>
2014, NeuroscienceCitation Excerpt :Cultures of nucleus ambiguus and NTS neurons maintained in ∼60 Torr (8 kPa) O2 grew well and were shown to have spontaneous pacemaker-like activity while exposure to 600 Torr (80 kPa) resulted in hyperpolarization and a complete lack of spontaneous activity (Rigatto et al., 1992, 1994). Historically, the rationale for using 95% O2 as the control O2 level for brain slice studies was to maintain tissue slice oxygenation at all depths in the tissue slice by (1) offsetting the reduced O2 carrying capacity of ACSF, which lacks hemoglobin, and (2) to overcome the diffusion barrier of a tissue slice lacking a perfused vasculature system (Bingmann and Kolde, 1982; Fujii et al., 1982; Reid et al., 1988; Aitken et al., 1995). Is 95% O2 required to accomplish both tasks however?
Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions
2012, Journal of Neuroscience MethodsCitation Excerpt :Most researchers in this field perfuse their slices continuously with fresh, carbogenated artificial cerebrospinal fluid (ACSF) in order to maintain pH balance and oxygen delivery to the tissue. Static slice recording, where the ACSF is not perfused, was originally developed for the purpose of analyzing slice metabolic waste products (Reid et al., 1988). While static recording has been discussed anecdotally in wider contexts (Aitken et al., 1995); in practice it is seldom used or reported on in the literature.
The membrane chamber: A new type of in vitro recording chamber
2011, Journal of Neuroscience MethodsCitation Excerpt :The interface chamber however has some significant disadvantages. The two main ones being the fact that the slice can be compressed due to gravity which may change its physiology (Croning and Haddad, 1998) and that the turnover of aCSF is very slow (Reid et al., 1988; Zhang and Heinemann, 1992; Thomson et al., 2000; Wu et al., 2001; Hájos and Mody, 2009). Additionally, the upper surface of the slice is ‘dry’ (although in fact there is a thin film of solution due to meniscal and capillary forces).
Establishing a physiological environment for visualized in vitro brain slice recordings by increasing oxygen supply and modifying aCSF content
2009, Journal of Neuroscience MethodsArtificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
2022, Frontiers in Cellular Neuroscience