Distribution of messenger RNA for the vasopressin V1a receptor in the CNS of male and female rats

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Abstract

The distribution of cells expressing mRNA encoding a vasopressin V1a receptor (V1aR) was examined in Long-Evans male and female rats by in situ hybridization using a [35S] cRNA probe. Specific hybridization to the vasopressin V1aR mRNA was evident in cells of the frontal cortex, piriform cortex, internal granular layer and the medial, dorsal, ventral and lateral portion of the anterior olfactory nucleus, zona limitans of the islands of Calleja, suprachiasmatic nucleus, CA1, CA2, CA3 and dentate gyrus of the hippocampus, paraventricular hypothalamic nucleus, ventromedial hypothalamic nucleus, arcuate nucleus, lateral habenular nucleus, and the molecular and granular cell layers of the cerebellum. The cerebellum, olfactory nucleus and the dentate gyrus appeared to be the most intensely labeled areas, while all other areas exhibited a lower level of expression. The anatomical distribution and the amount (as measured by optical density) of V1aR mRNA labeling was identical between male and female rats. This indicates that unlike the vasopressin gene itself, the expression of the vasopressin V1aR mRNA does not exhibit sexual dimorphism. These data demonstrate a wide spread distribution in the expression of the vasopressin V1aR mRNA in the CNS of male and female rats. This information on the anatomical distribution of the V1aR mRNA when combined with data concerning the anatomical distribution of the V1a binding sites, provides new information on the possible pre- and post-synaptic location of these neuropeptide receptors.

Reference (55)

  • GuillonG. et al.

    Kinetic and pharmacological characterization of vasopressin membrane receptors from human kidney medulla: relation to adenylate cyclase activation

    Eur. J. Pharmacol.

    (1982)
  • LawrenceJ.A.M. et al.

    [3H] Arginine vasopressin binding to rat brain: a homogenate and autoradiographic study

    Brain Res.

    (1988)
  • MatsuguchiH. et al.

    Blood pressure and heart rate responses to microinjection of vasopressin into the nucleus tractus solitarious region of the rat

    Neuropharmacology

    (1982)
  • McKinleyM.J. et al.

    Sensors for antidiuresis and thirst: osmoreceptors or CSF sodium detectors?

    Brain Res.

    (1978)
  • MeibachR.C. et al.

    Efferent connections of the hippocampal formation in the rat

    Brain Res.

    (1977)
  • MeisenbergG. et al.

    Centrally mediated effects of neurophypophyseal hormones

    Neurosci. Biobehav. Rev.

    (1983)
  • MillerM.A. et al.

    Sex differences in vasopressin neurons in the bed nucleus of the stria terminalis by in situ hybridization

    Peptides

    (1989)
  • PhillipsP.A. et al.

    Localization of vasopressin binding sites in rat brain by in vitro autoradiography using a radioiodinated V1 receptor antagonists

    Neuroscience

    (1988)
  • SzotP. et al.

    Cytoplasmic and nuclear vasopressin RNA in hypothalamic and extrahypothalamic neurons of the Brattleboro rat: an in situ hybridization study

    Mol. Cell. Neurosci.

    (1992)
  • van LeeuwenF.W. et al.

    Quantitative light microscopic autoradiographic localization of binding sites labelled with [3H]vasopressin antagonists d(CH2)5Tyr(Me)VP in the rat brain, pituitary and kidney

    Neurosci. Lett.

    (1987)
  • BaertschiA.J. et al.

    A novel type of vasopressin receptor on anterior pituitary corticotrophs

    Endocrinology

    (1985)
  • BallaT.P. et al.

    Pressor-type vasopressin receptors in the adrenal cortex: properties of binding, effects on phosphoinositide metabolism and aldosterone secretion

    Endocrinology

    (1985)
  • BrintonR.E. et al.

    Regional distribution of putative vasopressin receptors in rat brain and pituitary by quantitative autoradiography

  • BroadwellR.D.

    Olfactory relationship of the telencephalon and diencephalon in the rabbit. II. An autoradiographic and horseradish peroxidase study of the efferent connections of the anterior olfactory nucleus

    J. Comp. Neurol.

    (1975)
  • ButlenD. et al.

    Structural requirements for activation of vasopressin-sensitive adenylate cyclase, hormone binding and antidiuretic actions: effects of highly potent analogs and competitive inhibitors

    Mol. Pharmacol.

    (1978)
  • CantauB. et al.

    Vasopressin binding to isolated rat hepatocytes and liver membranes: regulation by GTP and regulation to glycogen phosphorylase activiation

    J. Recept. Res.

    (1980)
  • de WiedD. et al.

    Central target for the behavioral effects of vasopressin neuropeptide

    Nature

    (1984)
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