Immunocytochemical detection of 5′-bromodeoxyuridine incorporation in the central nervous system of the mouse

doi:10.1016/0165-3806(89)90033-3

Developmental Brain Research

Volume 49, Issue 2, 1 October 1989, Pages 311-317

https://doi.org/10.1016/0165-3806(89)90033-3 Get rights and content

Abstract

In order to label postmitotic neuroblasts, pregnant mice received a single pulse of 5′-bromodeoxyuridine (BrdU) between embryonic days E11 and E17. Young adult offspring were stained for BrdU using a monoclonal antibody and the peroxidase-antiperoxidase method. The distribution of immunoreactive nuclei was very similar to the [³H]thymidine data reported in the literature. The pattern of labelled nuclei after injection at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. Lightly and heavily labelled nuclei, probably representing subsequent postmitotic generations, were distinguished in BrdU-immunoreacted preparations. These data indicate that BrdU can be used to map neuronal birthdates.

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Short communicationImmunocytochemical detection of 5′-bromodeoxyuridine incorporation in the central nervous system of the mouse

Abstract

Dev. Brain Res.

Dev. Brain Res.

Brain Res.

Dev. Brain Res.

Autoradiographic study of cell migration during histogenesis in the cerebral cortex in the mouse

Nature (Lond.)

Time of neuron origin in the hippocampal region

The effects of 5′-bromodeoxyuridine on fusion of the cranial neural folds in the mouse embryo

Teratology

Time of origin of corresponding cell classes in the cerebral cortex of normal and mutant reeler mice: an autoradiographic analysis

J. Comp. Neurol.

Time of neuron origin of the hippocampus and dentate gyrus of normal and reeler mutant mice: an autoradiographic analysis

J. Comp. Neurol.

Rapid fluorometric detection of drug resistant tumour cells

Br. J. Cancer

A flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine

Times of generation of glutamic acid decarboxylase immunoreactive neurons in mouse somatosensory cortex

J. Comp. Neurol.

An immunofluorescence method for monitoring DNA synthesis by flow cytometry

Cytometry

Short communication
Immunocytochemical detection of 5′-bromodeoxyuridine incorporation in the central nervous system of the mouse