Research paperCryopreservation of freshly isolated synaptosomes prepared from the cerebral cortex of rats
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Patient-Derived In Vitro Models of Microglial Function and Synaptic Engulfment in Schizophrenia
2022, Biological PsychiatryCitation Excerpt :Following homogenization and fractionation (125), synaptosomal preparations are enriched in isolated pre- and postsynaptic components retaining their in vivo functions, including synaptic mitochondrial bioenergetics and respiration (126,127), neurotransmitter release (including glutamate), and endocytosis activities (123,128,129), and contain synaptic nucleotides including noncoding circular RNAs (130) and microRNAs (131). In addition, synaptosomal preparations can be cryopreserved while retaining their activity (132) to be banked as a ready-to-use reagent for downstream assays. Synaptosomes derived from postmortem patients have been used to model schizophrenia for the study of synaptic-specific protein levels and RNA expression specifically in synapses (131,133), aberrant synaptic protein content (134,135), synaptic proteomic studies (136,137), glutamate release (138,139), and complement deposition (140).
Superfusion of synaptosomes to study presynaptic mechanisms involved in neurotransmitter release from rat brain
2001, Brain Research ProtocolsAconitum sp. alkaloids: The modulation of voltage-dependent Na<sup>+</sup> channels, toxicity and antinociceptive properties
1997, European Journal of PharmacologyAnticonvulsive action of (±)-kavain estimated from its properties on stimulated synaptosomes and Na<sup>+</sup> channel receptor sites
1996, European Journal of Pharmacology