Letter to the editorsToxicity of light-exposed Hepes media
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2021, Food ChemistryCitation Excerpt :The oxidative instability of HEPES and similar organic buffers has been noted previously (Kirsch, Lomonosova, Korth, Sustmann, & de Groot, 1998) and can account for reports of production of H2O2 in cell media containing HEPES (Gery, 1985). In support, HEPES-mediated phototoxicity (ambient light) was associated with the production of H2O2 (Gery, 1985; Lepe-Zuniga et al., 1987). The superior efficacy of production of H2O2 by PIPES (Fig. 2a) which contains 2 sulfonic acid groups, versus other Good’s buffers, implicates that the sulfonic acid functional group is responsible for reduction of molecular oxygen to H2O2.
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