Only ‘de novo’ long-term depression (LTD) in the rat hippocampus in vitro is blocked by the same low concentration of FK506 that blocks LTD in the visual cortex

doi:10.1016/0006-8993(95)01168-4

Brain Research

Volume 705, Issues 1–2, 24 December 1995, Pages 241-246

https://doi.org/10.1016/0006-8993(95)01168-4 Get rights and content

Abstract

It has been proposed that the long-term depression (LTD) seen following low frequency stimulation (LFS) in the rat hippocampus involves calcineurin. We have tested this by examining the effect of FK506, a macrolide which blocks calcineurin at nanomolar concentrations, on synaptic transmission in the rat hippocampal slice at a concentration of 1 μM which has been shown to block LTD in the visual cortex. The effect of FK506 on long-term potentiation (LTP) and spontaneous transmitter release was also studied. The magnitude of LTD induced by LFS was 16.7 ± 2.4% in control which was not significantly different from the 22.3 ± 3.0% seen in the same preparations after exposure to FK506 for 25–30 min. In contrast the magnitude of LTD induced ‘de novo’ in preparations exposed to FK506 was significantly reduced. FK506 had no significant effect on LTP, miniature EPSP frequency, miniature EPSP amplitude, resting membrane potential or input resistance. These results, therefore, support the hypothesis that calcineurin is involved in ‘de novo’ LTD but it appears that an event is triggered by LFS whereby FK506-insensitive LTD can subsequently be activated by a second episode of LFS.

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Only ‘de novo’ long-term depression (LTD) in the rat hippocampus in vitro is blocked by the same low concentration of FK506 that blocks LTD in the visual cortex

Abstract

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Different voltage-dependent thresholds for inducing long-term depression and long-term potentiation in slices of rat visual cortex

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Long-term potentiation in the striatum is unmasked by removing the voltage-dependent magnesium block of NMDA receptor channels

Eur. J. Neurosci.

GABAA receptor function is regulated by phosphorylation in acutely dissociated guinea-pig hippocampal neurones

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Immunosuppressant FK506 enhances phosphorylation of nitric oxide synthase and protects against glutamate neurotoxicity

The role of protein kinase C substrate B-50 (GAP-43) in neurotransmitter release and long-term potentiation

Homosynaptic long-term depression in area CAI of hippocampus and effects of N-methyl-d-aspartate receptor blockade

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