Calbindin D-28k immunoreactivity in the rat accessory olfactory bulb

doi:10.1016/0006-8993(95)00547-4

Brain Research

Volume 689, Issue 1, 14 August 1995, Pages 93-100

https://doi.org/10.1016/0006-8993(95)00547-4 Get rights and content

Abstract

The distribution pattern and the morphology of calbindin D-28k-immunoreactive neurons were studied in the accessory olfactory bulb of the rat using a monoclonal antibody and the avidin-biotin-immunoperoxidase method. Positive neurons were observed in all layers but the vomeronasal nerve layer. Scarce monodendritic periglomerular neurons were calbindin D-28k-immunoreactive. Different morphological types of short-axon cells were calbindin D-28k-immunostained, with different degrees of intensity, in the boundary between the internal and external plexiform layer. In addition, deep short-axon cells located in the granule cell layer were calbindin D-28k-immunopositive. By contrast, previous studies described all cells in the rat accessory olfactory bulb as calbindin D-28k-immunonegative. The staining pattern in the rat accessory olfactory bulb showed both similarities and differences with the distribution pattern of the same calcium-binding protein in the main olfactory bulb.

Reference (50)

AlonsoJ.R. et al.
Calbindin D-28K and NADPH-diaphorase activity are localized in different populations of periglomerular cells in the rat olfactory bulb
J. Chem. Neuroanat.
(1993)
BaimbridgeK.G. et al.
Calcium-binding protein distribution in the rat brain
Brain Res.
(1982)
BaimbridgeK.G. et al.
Immunohistochemical localization of calcium-binding protein in the cerebellum, hippocampal formation and olfactory bulb of the rat
Brain Res.
(1982)
BarberP.C. et al.
An autoradiographic investigation of the projections of the vomeronasal organ to the accessory olfactory bulb in the mouse
Brain Res.
(1974)
BeanN.J. et al.
Vomeronasal organ removal and female mouse aggression: the role of experience
Physiol. Behav.
(1989)
CelioM.R. et al.
Neuroscience
(1990)
CelioM.R. et al.
Monoclonal antibodies directed against the calcium binding protein calbindin D-28k
Cell Calcium
(1990)
García-SeguraL.M. et al.
Immunohistochemical mapping of calcium-binding protein in the rat central nervous system
Brain Res.
(1984)
GoodmanJ.H. et al.
Calbindin D-28k immunoreactivity and selective vulnerability to ischemia in the dentate gyrus of the developing rat
Brain Res.
(1993)
HalászN. et al.
Tyrosine hydroxylase and 28k-vitamin D-dependent calcium binding protein are localized in different subpopulations of periglomerular cells of the rat olfactory bulb
Neurosci. Lett.
(1985)

JohnsonE.W. et al.

Calbindin-like immunoreactivity in two peripheral chemosensory tissues of the rat: taste buds and the vomeronasal organ

Brain Res.

(1992)

KaterS.B. et al.

Calcium regulation of the neuronal growth cone

Trends Neurosci.

(1988)

KennedyM.B.

Regulation of neuronal function by calcium

Trends Neurosci.

(1989)

LehmanM.N. et al.

Vomeronasal and olfactory pathways to the amygdala controlling male hamster sexual behavior: autoradiographic and behavioral analyses

Brain Res.

(1982)

MerchenthalerI. et al.

Immunocytochemical localization of proenkephalin-derived peptides in the central nervous system of the rat

Neuroscience

(1986)

MugnainiE. et al.

Immunocytochemical localization of GABA neurons and dopamine neurons in the rat main and accessory olfactory bulbs

Neurosci. Lett.

(1984)

RogersJ.H. et al.

Calretinin and calbindin D-28k in rat brain: patterns of partial co-localization

Neuroscience

(1992)

SeroogyK.B. et al.

Somatostatin like immunoreactivity in rat main olfactory bulb: extent of coexistence with neuropeptide Y-, tyrosine hydroxylase- and vitamin D-dependent calcium binding protein-like immunoreactivities

Brain Res.

(1989)

SéquierJ.M. et al.

Localization of calbindin D-28k mRNA in rat tissues by in situ hybridization

Neurosci. Lett.

(1988)

SmithsonK.G. et al.

Supraoptic nucleus afferents from the accessory olfactory bulb: evidence from anterograde and retrograde tract tracing in the rat

Brain Res. Bull.

(1992)

StraussK.I. et al.

Nucleotide sequence of rat calretinin cDNA

Neurochem. Int.

(1993)

TakamiS. et al.

The morphology of GABA-immunoreactive neurons in the accessory olfactory bulb of rats

Brain Res.

(1992)

TeicherM.H. et al.

Olfactory and vomeronasal system mediation of maternal recognition in the developing rat

Brain Res.

(1984)

WysockiC.J.

Neurobehavioral evidence for the involvement of the vomeronasal system in mammalian reproduction

Neurosci. Biobehav. Rev.

(1979)

AlonsoJ.R. et al.

NADPH-diaphorase active and calbindin D-28k-immunoreactive neurons and fibers in the olfactory bulb of the hedgehog (Erinaceus europaeus)

J. Comp. Neurol.

(1995)

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Calbindin D28K immunoreactive neurons in vomeronasal organ and their projections to the accessory olfactory bulb in the rat
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Citation Excerpt :
Axons of these neurons terminate in the glomeruli of the accessory olfactory bulb (AOB). CB expression has been demonstrated in the rat AOB [19,31] and VNO [1,21] and in mouse VNO [24]. In this study we used antibodies against CB to immunostain the rat VNO and AOB and identified a novel pattern of projections from the CB-immunoreactive subpopulation of receptor neurons to a special group of glomeruli in the anterior AOB.
The vomeronasal system is a nasal chemosensory system involved in pheromone detection. The chemosensory receptor neurons are located in the sensory epithelium of the vomeronasal organ (VNO). Their axons terminate in the glomeruli of the accessory olfactory bulb (AOB). In this study, we examined the expression of calbindin D28k (CB) in the rat VNO and AOB. In the VNO, a subpopulation of receptor neurons in the middle layer of the sensory epithelium was immunostained with antibodies to CB. Their axons could be traced to terminate in a group of glomeruli in the anterior half of the AOB glomerular layer. This group of CB-immunostained glomeruli in the anterior half of the AOB included a few large glomeruli close to the boundary between the anterior and posterior halves of the AOB, and several small glomeruli scattered in the anterior region of the AOB glomerular layer. The positions of the CB-immunostained glomeruli in the AOB, especially those close to the anterior–posterior boundary, were similar in the two bulbs and in different rats. No sex difference was found. A developmental study showed that the CB-immunoreactive receptor neurons in the middle layer of the VNO sensory epithelium and CB-immunoreactive glomeruli in the anterior AOB were present on the 14th postnatal day and older. The distribution pattern of the CB-immunostained receptor neurons and their localized projection suggest the possibility that these neurons may express the same or functionally related pheromone receptor genes.
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Several developments during the past 15 years have profoundly affected our understanding of the vomeronasal system (VNS) of vertebrates. In the mid 1990s, the vomeronasal epithelium of mammals was found to contain two populations of receptor cells, based on their expression of G-proteins. These two populations of neurons were subsequently found to project their axons to different parts of the accessory olfactory bulb (AOB), forming the basis of segregated pathways with possibly heterogeneous functions. A related discovery was the cloning of members of at least two gene families of putative vomeronasal G-protein-coupled receptors (GPRs) in the vomeronasal epithelium. Ligand binding to these receptors was found to activate a phospholipase C (PLC)-dependent signal transduction pathway that primarily involves an increase in intracellular inositol-tris-phosphate and intracellular calcium. In contrast to what was previously believed, neuron replacement in the vomeronasal epithelium appears to occur through a process of vertical migration in most mammals. New anatomical studies of the central pathways of the olfactory and vomeronasal systems indicated that these two systems converge on neurons in the telencephalon, providing an anatomical substrate for functional interactions. Combined anatomical, physiological and behavioral studies in mice provided new information that furthered our understanding of one of the most striking pheromonal phenomena, the Bruce effect. Finally, contrary to prior observations, new anatomical studies indicated that a vomeronasal organ (VNO) was present in human adults and reports were published indicating that this system might be functional. These latter observations are still controversial and require confirmation from independent laboratories.
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The distribution and morphology of neurocalcin-immunopositive neurons have been studied in the rat accessory olfactory bulb. Different subsets of neurons displaying neurocalcin immunoreactivity were found in the glomerular layer, the external plexiform layer and the internal plexiform layer. The most abundant staining was detected in the glomerular layer where neurocalcin-immunoreactive periglomerular cells and external tufted cells were observed in the lateral glomeruli, whereas the central region of this layer was practically devoid of immunopositive neurons. In the external plexiform layer, medial tufted cells and Van Gehuchten cells displayed neurocalcin immunoreactivity. In the internal plexiform layer, interneurons classified as horizontal cells and vertical cells of Cajal were neurocalcin-immunostained. The staining pattern for neurocalcin in the accessory olfactory bulb showed similarities with the immunostaining described in this brain region for another EF-hand calcium binding protein, calbindin D-28k. However, after double immunohistochemical labeling, colocalization of both proteins in the same neuron was not observed, reflecting a biochemical heterogeneity within morphologically homogeneous neuronal groups.
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Calbindin D-28k immunoreactivity in the rat accessory olfactory bulb

Abstract

J. Chem. Neuroanat.

Brain Res.

Brain Res.

Brain Res.

Physiol. Behav.

Neuroscience

Cell Calcium

Brain Res.

Brain Res.

Neurosci. Lett.

Brain Res.

Trends Neurosci.

Trends Neurosci.

Brain Res.

Neuroscience

Neurosci. Lett.

Neuroscience

Brain Res.

Neurosci. Lett.

Brain Res. Bull.

Neurochem. Int.

Brain Res.

Brain Res.

Neurosci. Biobehav. Rev.

NADPH-diaphorase active and calbindin D-28k-immunoreactive neurons and fibers in the olfactory bulb of the hedgehog (Erinaceus europaeus)

J. Comp. Neurol.