[3H]MK-801 binding to the NMDA receptor complex, and its modulation in human frontal cortex during development and aging

doi:10.1016/0006-8993(92)91586-4

Brain Research

Volume 588, Issue 2, 21 August 1992, Pages 277-286

https://doi.org/10.1016/0006-8993(92)91586-4 Get rights and content

Abstract

[³H]MK-801 binding was found to decline with age in well washed membranes from human frontal cortex taken from an age series from 24 weeks gestation to 100 years old. The decline was significant under basal conditions (no added modulators) (P < 0.01), and highly significant under stimulation with glutamate, glycine and spermidine alone and in combination (P < 0.001). Scatchard analysis in the presence of glutamate and glycine showed this decline was due to a loss in the number [³H]MK-801 binding sites rather than a change in the affinity of the binding site. There was a highly significant age related reduction in the attenuation of [³H]MK-801 binding by zinc (P < 0.001). In foetal and neonatal cases up to 7 weeks of age spermidine behaved in an antagonistic manner, inhibiting rather than stimulating [³H]MK-801 binding, when alone or in the presence of glutamate and glycine. The changes in influence of glutamate, glycine, spermidine and zinc on [³H]MK-801 binding during development and aging were not due to other pre- or postmortem factors. The reverse effect of spermidine in the foetal and neonatal cases has therapeutic implications in the treatment of neonates with antiischaemic agents whose action involves the polyamine site.

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Research report[3H]MK-801 binding to the NMDA receptor complex, and its modulation in human frontal cortex during development and aging

Abstract

Trends Pharmacol.

Trends Neurosci.

Int. J. Dev. Neurosci.

Dev. Brain Res.

Neurobiol. Aging

Neuroscience

Trends Pharmacol.

Neurosci. Lett.

J. Biol. Chem.

Brain Res. Rev.

Exp. Neurol.

Trends Pharmacol.

Neurosci. Lett.

Eur. J. Pharmacol.

Neurosci. Lett.

Brain Res.

Brain Res.

Dev. Brain Res.

Neurobiol. Aging

An age comparison of the rates of aquisition and forgetting of spatial infoormation in relation to long term enhancement of hippocampal synapses

Behav. Neurosci.

Structural changes in the aging nervous system

Ifenprodil and SL 82.0715 as cerebral antiischaemic agents III. Evidence for antagonistic effects at the polyamine modulatory site within the N-methyl-d-aspartate receptor complex

J. Pharmacol. Exp. Ther.

Effect of zinc on NMDA receptor-mediated channel currents in cortical neurons

J. Neurosci.

Neuron numbers and sizes in aging brain: comparisons of human, monkey, and rodent data

Neurobiol. Aging

The novel anticonvulsant MK-801 binds to the activated state of the N-methyl-d-aspartate receptor in rat brain

Br. J. Pharmacol.

Research report
[³H]MK-801 binding to the NMDA receptor complex, and its modulation in human frontal cortex during development and aging

Ifenprodil and SL 82.0715 as cerebral antiischaemic agents III. Evidence for antagonistic effects at the polyamine modulatory site within the $N- methyl- d -aspartate$ receptor complex

The novel anticonvulsant MK-801 binds to the activated state of the $N- methyl- d -aspartate$ receptor in rat brain