Abstract
Proper formation of neuronal dendritic branching is crucial for correct brain function. The number and distribution of receptive synaptic contacts are defined by the size and shape of dendritic arbors. Our previous research found that protocadherin 11 X-linked protein (Pcdh11x) is predominantly expressed in neurons and has an influence on dendritic branching. In this study, gain-of-function and loss-of-function experiments revealed that Pcdh11x acts as a negative regulator of dendritic branching in cultured cortical neurons derived from embryonic day 16 mice. Overexpression of wild-type Pcdh11x (Pcdh11x-GFP) reduced dendritic complexity, whereas knockdown of Pcdh11x increased dendritic branching. It was further demonstrated that Pcdh11x activates PI3K/AKT signaling to negatively regulate dendritic branching.
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Acknowledgments
This study was funded by Military Medical Project (BWS11J002, BWS12J010) and National Natural Science Foundation (No. 81401031). We thank Springer Language Editing (Project No.: C1501-40452-WuCuiying) for assisting in the preparation of this manuscript. The authors would like to thank all the anonymous reviewers for their valuable comments on how to improve the quality of this paper.
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No conflicts of interest, financial or otherwise, are declared by the authors.
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Wu, C., Niu, L., Yan, Z. et al. Pcdh11x Negatively Regulates Dendritic Branching. J Mol Neurosci 56, 822–828 (2015). https://doi.org/10.1007/s12031-015-0515-8
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DOI: https://doi.org/10.1007/s12031-015-0515-8