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Beneficial Effect of Interleukin-1 Receptor Antagonist Protein on Spinal Cord Injury Recovery in the Rat

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Abstract

We assessed the effect of treatment with the interleukin-1 receptor antagonist protein (IRAP) on morphological and functional recovery in a rat model of SCI. All sections were processed for immunohistochemistry, hematoxylin–eosin, and Nissl staining. Rats were assessed for hind limb motor function using the Basso, Beattie, and Bresnahan (BBB) hind limb locomotor rating scale and the inclined plane test. At 1, 48, and 72 h after operation, there was a significant increase in neurofilament proteins and brain-derived neurotrophic factor expression in the IRAP group I when compared with the saline group I and the sham-operated group I (P < 0.05). The mean inclined plane scores and BBB scores for the IRAP group II were higher than the saline group II at 1, 2, 3, and 4 weeks post-injury (P < 0.05). In conclusion, treatment with IRAP enhanced neuronal survival after SCI.

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REFERENCES

  1. Sud, S., S.Y. Yang, C.H. Evans, P.D. Robbins, and P.H. Wooley. 2001. Effects of cytokine gene therapy on particulate-induced inflammation in the murine air pouch. Inflammation 25: 361–372.

    Article  PubMed  CAS  Google Scholar 

  2. Bravo, G., R. Rojas-Martínez, F. Larios, E. Hong, G. Castañeda-Hernández, G. Rojas, and G. Guízar-Sahagún. 2001. Mechanisms involved in the cardiovascular alterations immediately after spinal cord injury. Life Sciences 68: 1527–1534.

    Article  PubMed  CAS  Google Scholar 

  3. Huang, H., S. Fan, X. Ji, Y. Zhang, F. Bao, and G. Zhang. 2009. Recombinant human erythropoietin protects against experimental spinal cord trauma injury by regulating expression of the proteins MKP-1 and p-ERK. The Journal of International Medical Research 37: 511–519.

    PubMed  CAS  Google Scholar 

  4. Tzeng, S.F., H. Cheng, Y.S. Lee, J.P. Wu, B.J. Hoffer, and J.S. Kuo. 2001. Expression of neural cell adhesion molecule in spinal cords following a complete transection. Life Sciences 68(9): 1005–1012.

    Article  PubMed  CAS  Google Scholar 

  5. Johnson, R.W., S. Arkins, R. Dantzer, and K.W. Kelley. 1997. Hormones, lymphohemopoietic cytokines and the neuroimmune axis. Comparative Biochemistry and Physiology. Part A, Physiology 116: 183–201.

    Article  PubMed  CAS  Google Scholar 

  6. Xu, Z., B.R. Wang, X. Wang, F. Kuang, X.L. Duan, X.Y. Jiao, and G. Ju. 2006. ERK1/2 and p38 mitogen-activated protein kinase mediate iNOS-induced spinal neuron degeneration after acute traumatic spinal cord injury. Life Sciences 79: 1895–1905.

    Article  PubMed  CAS  Google Scholar 

  7. Rothwell, N.J., and G.N. Luheshi. 2000. Interleukin 1 in the brain: biology, pathology and therapeutic target. Trends in Neurosciences 23: 618–625.

    Article  PubMed  CAS  Google Scholar 

  8. Wang, X.F., L.D. Huang, P.P. Yu, J.G. Hu, L. Yin, L. Wang, X.M. Xu, and P.H. Lu. 2006. Upregulation of type I interleukin-1 receptor after traumatic spinal cord injury in adult rats. Acta Neuropatho 111: 220–228.

    Article  CAS  Google Scholar 

  9. Baamonde, A., V. Curto-Reyes, L. Juárez, A. Meana, A. Hidalgo, and L. Menéndez. 2007. Antihyperalgesic effects induced by the IL-1 receptor antagonist anakinra and increased IL-1beta levels in inflamed and osteosarcoma-bearing mice. Life Sciences 81: 673–682.

    Article  PubMed  CAS  Google Scholar 

  10. Rohleder, N., J.M. Wolf, I. Herpfer, B.L. Fiebich, C. Kirschbaum, and K. Lieb. 2006. No response of plasma substance P, but delayed increase of interleukin-1 receptor antagonist to acute psychosocial stress. Life Sciences 78: 3082–3089.

    Article  PubMed  CAS  Google Scholar 

  11. Wu, J., H. Yang, Z. Qiu, Q. Zhang, T. Ding, and D. Geng. 2010. Effect of combined treatment with methylprednisolone and Nogo-A monoclonal antibody after rat spinal cord injury. The Journal of International Medical Research 38: 570–582.

    PubMed  CAS  Google Scholar 

  12. Khan, T., R.M. Havey, S.T. Sayers, A. Patwardhan, and W.W. King. 1999. Animal models of spinal cord contusion injuries. Laboratory Animal Science 49: 161–172.

    PubMed  CAS  Google Scholar 

  13. Maharajh, G.S., E.A. Pascoe, W.C. Halliday, H.P. Grocott, D.B. Thiessen, L.G. Girling, M.S. Cheang, and W.A. Mutch. 1996. Neurological outcome in a porcine model of descending thoracic aortic surgery. Left atrial-femoral artery bypass versus clamp/repair. Stroke 27: 2095–2100.

    Article  PubMed  CAS  Google Scholar 

  14. Panico, A.M., P. Vicini, G. Massimo, V. Cardile, B. Gentile, S. Avondo, F. Vittorio, and G. Ronsisvalle. 2004. Protective effects of benzisothiazolylamidines on IL-1 beta induced alterations in human articular chondrocyte metabolism. Inflammation 28: 231–235.

    Article  PubMed  CAS  Google Scholar 

  15. Sanchez, C.P., and Y.Z. He. 2002. Alterations in the growth plate cartilage of rats with renal failure receiving corticosteroid therapy. Bone 30: 692–698.

    Article  PubMed  CAS  Google Scholar 

  16. Yao, H.W., J. Li, J.Q. Chen, and S.Y. Xu. 2004. A 771726, the active metabolite of leflunomide, inhibits TNF-alpha and IL-1 from Kupffer cells. Inflammation 28: 97–103.

    Article  PubMed  CAS  Google Scholar 

  17. Theodorou, V., J. Fioramonti, and L. Bueno. 1993. Recombinant interleukin-1 receptor antagonist protein prevents sensitization and intestinal anaphylaxis in guinea pigs. Life Sciences 53: 733–738.

    Article  PubMed  CAS  Google Scholar 

  18. Fitch, M.T., C. Doller, C.K. Combs, G.E. Landreth, and J. Silver. 1999. Cellular and molecular mechanisms of glial scarring and progressive cavitation: In vivo and in vitro analysis of inflammation-induced secondary injury after CNS trauma. Journal of Neuroscience 19: 8182–8198.

    PubMed  CAS  Google Scholar 

  19. Rapalino, O., O. Lazarov-Spiegler, E. Agranov, G.J. Velan, E. Yoles, M. Fraidakis, A. Solomon, R. Gepstein, A. Katz, M. Belkin, M. Hadani, and M. Schwartz. 1998. Implantation of stimulated homologous macrophages results in partial recovery of paraplegic rats. Nat Med 4: 814–821.

    Article  PubMed  CAS  Google Scholar 

  20. Kamei, N., N. Tanaka, Y. Oishi, T. Hamasaki, K. Nakanishi, N. Sakai, and M. Ochi. 2007. BDNF, NT-3, and NGF released from transplanted neural progenitor cells promote corticospinal axon growth in organotypic cocultures. Spine 32: 1272–1278.

    Article  PubMed  Google Scholar 

  21. Sasaki, M., C. Radtke, A.M. Tan, P. Zhao, H. Hamada, K. Houkin, O. Honmou, and J.D. Kocsis. 2009. BDNF-hypersecreting human mesenchymal stem cells promote functional recovery, axonal sprouting, and protection of corticospinal neurons after spinal cord injury. The Journal of Neuroscience 29: 14932–14941.

    Article  PubMed  CAS  Google Scholar 

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ACKNOWLEDGMENTS

This study was supported by Dr. Start Fund of Guangxi Medical University (308010) and Research Foundation of Guangxi Education Department (Gui Education200710LX063).

Conflict of Interest Statement

The authors declare that there are no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Spine Osteopathia, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People’s Republic of China

    Shaohui Zong

  2. College of Public Hygiene of Guangxi Medical University, No. 22 Shuangyong Road, Nanning, 530021, Guangxi, People’s Republic of China

    Gaofeng Zeng & Bo Wei

  3. Graduate School of Guangxi Medical University, Nanning, Guangxi, People’s Republic of China

    Chunxiang Xiong & Yuxi Zhao

Authors
  1. Shaohui Zong
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  2. Gaofeng Zeng
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  3. Bo Wei
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  4. Chunxiang Xiong
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  5. Yuxi Zhao
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Corresponding author

Correspondence to Bo Wei.

Additional information

Gaofeng Zeng contributed equally to this work and should be considered as co-first authors.

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Zong, S., Zeng, G., Wei, B. et al. Beneficial Effect of Interleukin-1 Receptor Antagonist Protein on Spinal Cord Injury Recovery in the Rat. Inflammation 35, 520–526 (2012). https://doi.org/10.1007/s10753-011-9341-5

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  • DOI: https://doi.org/10.1007/s10753-011-9341-5

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