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Stratified organization and disorganization of inner plexiform layer revealed by TNAP activity in healthy and diabetic rat retina

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Abstract

Tissue non-specific alkaline phosphatase (TNAP), an abundant ectophosphatase, is present in various organs including the brain and retina of several vertebrate species. Evidence is emerging that TNAP influences neural functions in multiple ways. In rat, strong TNAP activity has been found in retinal vessels, photoreceptors, and both synaptic layers. In the present study, we identified eleven strata of the inner plexiform layer (IPL) by using TNAP histochemistry alone. The TNAP strata corresponded exactly to the strata seen after combined immunohistochemistry with four canonical IPL markers (TH-ChAT-CR-PKCα). Therefore, as described in other mammalian species, our data support the existence of multiple morphologically and functionally discernible IPL strata in rats. Remarkably, the stratification pattern of the IPL was severely disrupted in a diabetic rat model, even before changes in the canonical IPL markers were detectable. These findings indicate that TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration.

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Acknowledgments

This work is dedicated to the blessed memory of our dear colleague, Dr. Tamás Görcs. The authors wish to thank Zsuzsanna Vidra for her technical help and are grateful to Dr. Mark Eyre for assistance with the English language.

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58, H-1094, Budapest, Hungary

    Orsolya Kántor, Alexandra Varga, Róbert Tóth, Emese Pálfi, Andrea Székely & László Négyessy

  2. Department of Ophthalmology, Semmelweis University, Budapest, H-1085, Hungary

    Anna Énzsöly

  3. Department of Human Morphology and Developmental Biology, Semmelweis University, Budapest, H-1094, Hungary

    Anna Énzsöly, Ágoston Szél & Ákos Lukáts

  4. Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs, H-7624, Hungary

    Tamás Kovács-Öller & Béla Völgyi

  5. János Szentágothai Research Center, Pécs, H-7624, Hungary

    Tamás Kovács-Öller & Béla Völgyi

  6. Life Imaging Center, Center for Biological Systems Analysis, Albert-Ludwigs University, Freiburg, 79104, Germany

    Roland Nitschke

  7. BIOSS Centre for Biological Signaling Studies, Albert-Ludwigs-University Freiburg, Freiburg, 79104, Germany

    Roland Nitschke

  8. Complex Systems and Computational Neuroscience Group, Wigner Research Center for Physics, Hungarian Academy of Sciences, Budapest, H-1121, Hungary

    László Négyessy & Zoltán Somogyvári

Authors
  1. Orsolya Kántor
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  2. Alexandra Varga
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  4. Anna Énzsöly
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  5. Emese Pálfi
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  6. Tamás Kovács-Öller
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  7. Roland Nitschke
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  8. Ágoston Szél
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  9. Andrea Székely
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  10. Béla Völgyi
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  11. László Négyessy
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  12. Zoltán Somogyvári
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  13. Ákos Lukáts
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Corresponding author

Correspondence to Orsolya Kántor.

Additional information

This study was supported by the Hungarian National Research Fund (OTKA NN79366 to L.N., OTKA K105247 to B.V., OTKA K73000 to Á.S.), the European Union, and the State of Hungary and was co-financed by the European Social Fund in the framework of the National Excellence Program (TÁMOP-4.2.4.A/2-11/1-2012-0001 to T.K-Ö. and B.V., TÁMOP-4.2.1B-09/1KMRB2010-0001 to Á.S.).

Zoltán Somogyvári and Ákos Lukáts contributed equally to this work.

The manuscript contains no clinical studies or patient data.

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Kántor, O., Varga, A., Tóth, R. et al. Stratified organization and disorganization of inner plexiform layer revealed by TNAP activity in healthy and diabetic rat retina. Cell Tissue Res 359, 409–421 (2015). https://doi.org/10.1007/s00441-014-2047-x

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