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High-resolution comparative mapping of pig Chromosome 4, emphasizing the FAT1 region

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Abstract

The first quantitative trait locus (QTL) in pigs, FAT1, was found on Chromosome 4 (SSC4) using a Wild Boar intercross. Further mapping has refined the FAT1 QTL to a region with conserved synteny to both human Chromosomes 1 and 8. To both improve the comparative map of the entire SSC4 and to define the specific human chromosome region with conserved synteny to FAT1, we have now mapped 103 loci to pig Chromosome 4 using a combination of radiation hybrid and linkage mapping. The physical data and linkage analysis results are in very good agreement. Comparative analysis revealed that gene order is very well conserved across SSC4 compared to both HSA1 and HSA8. The breakpoint in conserved synteny was refined to an area of about 23 cR on the q arm of SSC4 corresponding to a genetic distance of less than 0.5 cM. Localizations of the centromeres do not seem to have been conserved between the two species. No remnants of the HSA1 centromere were detected on the corresponding region on SSC4 and traces from the centromeric region of SSC4 cannot clearly be revealed on the homologous region on HSA8. This refined SSC4 map and the comparative analysis will be a great aid in the search for the genes underlying the FAT1 locus.

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Acknowledgments

Hanna Karlsson is acknowledged for expert technical assistance, Katie Barry for selection of ESTs, and Brad Edeal for RH mapping. This work was supported by USDA-NRICGP 99-35205-8370, the Iowa Agriculture and Home Economics Experiment Station, Ames, IA, Project No. 3596, the University of Nebraska Agricultural Research Division (Lincoln, NE; Journal Series No. 14396), and Hatch funds to Iowa and Nebraska. The work has also been supported by the Foundation for Strategic Research, The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, and Arexis AB. ALA and SIA acknowledge support from the UK Biotechnology and Biological Sciences Research Council.

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  1. Maria Moller

    Present address: Experimental Medicine Unit, Swansea Clinical School, University of Wales, Singleton Park, SA2 8PP, Swansea, Swansea, Wales

Authors and Affiliations

  1. Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, 75124, Uppsala, Sweden

    Maria Moller, Frida Berg & Leif Andersson

  2. Laboratoire de Génétique Cellulaire, Institut National de la Recherche Agronomique (INRA), BP 27, 31326, Castanet-Tolosan, France

    Juliette Riquet, Katia Feve & Denis Milan

  3. Department of Animal Science, University of Nebraska, Lincoln, Nebraska, 68583, USA

    Daniel Pomp

  4. Department of Genomics and Bioinformatics, Roslin Institute, Midlothian, EH25 9PS, Scotland, UK

    Alan Archibald & Susan Anderson

  5. Department of Animal Science and Center for Integrated Animal Genomics, Iowa State University, 225 Kildee Hall, Ames, Iowa, 50011, USA

    Yuandan Zhang, Max Rothschild & Christopher K. Tuggle

  6. Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, 75124, Uppsala, Sweden

    Leif Andersson

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  1. Maria Moller
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Correspondence to Christopher K. Tuggle.

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Moller, M., Berg, F., Riquet, J. et al. High-resolution comparative mapping of pig Chromosome 4, emphasizing the FAT1 region. Mamm Genome 15, 717–731 (2004). https://doi.org/10.1007/s00335-004-2366-4

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