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Immunohistochemistry of biogenic polypeptides in nerve cells and fibres of the guinea pig inferior mesenteric ganglion after perturbations

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Summary

Immunohistochemistry of peptide-and dopamine-β-hydroxylase-(DBH)-containing varicose nerve fibres and ganglion cells, respectively, in the guinea pig inferior mesenteric ganglion was investigated followinga) transsection of mesenteric (colonic) branches,b) transsection of central (lumbar splanchnic, intermesenteric and hypogastric) branches, andc) transplantation into the spleen.

The findings indicate that pathways of different opioid peptides are not identical Met-enkephalin-and met-enkephalin-arg-phe-(cleavage products from pre-proenkephalin) containing fibres course in central branches to make contact in the inferior mesenteric ganglion. Dynorphin-and α-neo-endorphin-(deriving from pre-prodynorphin) containing fibers as well as leu-enkephalin-(included in the dynorphin sequence) fibres appear to rise not only from central and from enteric somata, but also from intraganglionic noradrenergic neurons. Similar pathways seem to be used by VIP-and by neurotensin-immunoreactive cell bodies are rare. Practically all substance P-and most CGRP-immunoreactive fibres enter the ganglion via central branches and, to a large extent, traverse it, but some CGRP-immunoreactive influx appears to come from the intestine. The origin of intraganglionic substance P-and CGRP-immunoreactive fibres after ganglion transplantation remained unidentified. Somatostatin-and neuropeptide Y-immunoreactive fibres predominantly have an intraganglionic origin as have DBH-immunoreactive noradrenergic fibres. The demonstrated alterations in neuropeptide immunoreactivity of intraganglionic and periganglionic nerve fibres following the applied transsection procedures contribute to the present knowledge on origin and destination of peptidergic transmitter segments in the guinea pig inferior mesenteric ganglion. Moreover, the present study provides evidence that intrinsic participitation in intraganglionic fibre supply is more extensive than hitherto believed.

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Authors and Affiliations

  1. Department of Anatomical Sciences, Faculty of Health Sciences, State University of New York, 319 Farber Hall, 14214, Buffalo, NY, USA

    R. H. Webber

  2. Institute I of Anatomy, University of Heidelberg, Im Neuenheimer Feld 307, D-6900, Heidelberg, Federal Republic of Germany

    Ch. Heym

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  1. R. H. Webber
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  2. Ch. Heym
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Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday

Supported by the Deutsche Forschungsgemeinschaft, grant He 919/6-2

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Webber, R.H., Heym, C. Immunohistochemistry of biogenic polypeptides in nerve cells and fibres of the guinea pig inferior mesenteric ganglion after perturbations. Histochemistry 88, 287–297 (1988). https://doi.org/10.1007/BF00570286

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