Summary
The distributions within the coeliac ganglion of different chemically coded subgroups of noradrenaline neurons, and the relationships between these neurons and nerve fibres projecting to the ganglion from the intestine, have been assessed quantitatively by use of an immunohistochemical double-staining method. Noradrenaline (NA) neurons made up 99% of all cell bodies. Of these, 21% were also reactive for somatostatin (NA/SOM neurons), 53% were also reactive for NPY (NA/NPY neurons), and 26% were not reactive for either peptide. NA neurons without reactivity for any of the peptides whose localization was tested have been designated NA/-. A small percentage, about 1%, of neurons were reactive for both NPY and SOM. The three major types of NA neurons were arranged in clumps or ribbons throughout the ganglia, with a tendency for NA/SOM neurons to be medial and NA/NPY neurons to be lateral in the ganglia. A small group of neurons (<1%) encoded with dynorphin, NPY and vasoactive intestinal peptide (VIP) was encountered. VIP-immunoreactive nerve terminals, projecting to the ganglion from cell bodies in the intestine, ended around NA/SOM and NA/neurons but not around NA/NPY neurons. Thus, the VIP axons from the intestine end selectively around neurons that modify intestinal function (NA/SOM and NA/-neurons) but not around neurons, the terminals of which supply blood vessels (NA/NPY neurons).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Böck P (1982) The Paraganglia. Springer-Verlag, Berlin
Bowers CW, Zigmond RE (1979) Localization of neurons in the rat superior cervical ganglion which project into different postganglionic trunks. J Comp Neurol 185:381–392
Costa M, Furness JB (1973) Observations on the anatomy and amine histochemistry of the nerves and ganglia which supply the pelvic viscera and on the associated chromaffin tissue in the guinea-pig. Z Anat Entwickl-Gesch 140:85–108
Costa M, Furness JB (1983) The origins, pathways and terminations of neurons with VIP-like immunoreactivity in the guinea-pig small intestine. Neuroscience 8:665–676
Costa M, Furness JB (1984) Somatostatin is present in a subpopulation of noradrenergic nerve fibres supplying the intestine. Neuroscience 13:911–920
Costa M, Furness JB, Llewellyn-Smith IJ, Davies B, Oliver J (1980) An immunohistochemical study of the projections of somatostatin-containing neurons in the guinea-pig intestine. Neuroscience 5:841–852
Costa M, Furness JB, Yanaihara N, Yanaihara C, Moody TW (1984) Distribution and projections of neurons with immunoreactivity for both gastrin-releasing peptide and bombesin in the guinea-pig small intestine. Cell Tissue Res 235:285–293
Dalsgaard C-J, Hökfelt T, Schultzberg M, Lundberg JM, Terenius L, Dockray G, Goldstein M (1983) Origin of peptide-containing fibres in the inferior mesenteric ganglion of the guinea-pig: immunohistochemical studies with antisera to substance P, enkephalin, vasoactive intestinal polypeptide, cholecystokinin and bombesin. Neuroscience 9:191–211
Eränkö O (1976) SIF cells. Structure and function of the small, intensely fluorescent sympathetic cells. Fogarty International Center Proceedings No. 30, DHEW Publications, Washington D.C.
Furness JB, Costa M (1974) The adrenergic innervation of the gastrointestinal tract. Ergeb Physiol 69:1–54
Furness JB, Costa M, Walsh JH (1981) Evidence for and significance of the projections of VIP neurons from the myenteric plexus to the taenia coli in the guinea-pig. Gastroenterology 80:1557–1561
Furness JB, Costa M, Keast JR (1984) Choline acetyltransferaseand peptide immunoreactivity of submucous neurons in the small intestine of the guinea-pig. Cell Tissue Res 237:328–336
Furness JB, Costa M, Gibbins IL, Llewellyn-Smith IJ, Oliver JR (1985) Neurochemically similar myenteric and submucous neurons directly traced to the mucosa of the small intestine. Cell Tissue Res 241:155–163
Hökfelt T, Elfvin L-G, Elde R, Schultzberg M, Goldstein M, Luft R (1977) Occurrence of somatostatin-like immunoreactivity in some peripheral sympathetic noradrenergic neurons. Proc Natl Acad Sci USA 74:3587–3591
Job C, Lundberg A (1952) Reflex excitation of cells in the inferior mesenteric ganglion on stimulation of the hypogastric nerve. Acta Physiol Scand 26:366–382
Keast JR, Furness JB, Costa M (1984) Somatostatin in human enteric nerves: Distribution and characterisation. Cell Tissue Res 237:299–308
Kuntz A, Saccomanno GJ (1944) Reflex inhibition of intestinal motility mediated through decentralized prevertebral ganglia. J Neurophysiol 7:163–170
Larsson L-I, Rehfeld JF (1979) Localization and molecular heterogeneity of cholecystokinin in the central and peripheral nervous system. Brain Res 165:201–218
Lichtman JW, Purves D, Yip JW (1979) On the purpose of selective innervation of guinea-pig superior cervical ganglion cells. J Physiol (London) 292:69–84
Lundberg JM, Hökfelt T, Anggard A, Kimmel J, Goldstein M, Markey K (1980) Coexistence of an avian pancreatic polypeptide (APP) immunoreactive substance and catecholamine in some peripheral and central neurons. Acta Physiol Scand 110:107–109
Lundberg JM, Hökfelt T, Anggard A, Terenius L, Elde R, Markey K, Goldstein M (1982) Organization principles in the peripheral sympathetic nervous system: Subdivision by coexisting peptides (Somatostatin, avian pancreatic polypeptide-, and vasoactive intestinal polypeptide-like immunoreactivities). Proc Natl Acad Sci USA 79:1303–1307
Maccarrone C, Jarrott B (1985) Differences in regional brain concentrations of neuropeptide Y in spontaneously hypertensive (SH) and Wistar-Kyoto (WKY) rats. Brain Res 345:165–169
McLennan H, Pascoe JH (1954) The origin of certain non-medullated nerve fibres which form synapses in the inferior mesenteric ganglion of the rabbit. J Physiol (London) 124:145–156
Miller RJ, Chang KJ, Cooper B, Cuatrecasas P (1978) Radioimmunoassay and characterization of enkephalins in rat tissues. J Biol Chem 253:531–538
Morris JL, Gibbins IL, Furness JB, Costa M, Murphy R (1985) Co-localization of NPY, VIP and dynorphin, in non-noradrenergic axons of the guinea-pig uterine artery. Neurosci Letts 62:31–37
Purves D (1975) Functional and structural changes in mammalian sympathetic neurons following interruption of their axons. J Physiol (London) 252:429–463
Purves D (1976) Functional and structural changes in mammalian sympathetic neurones following colchicine application to postganglionic nerves. J Physiol (London) 259:159–175
Shively MJ, Stump JE (1975) The systemic arterial pattern of the guinea pig: the abdomen. Anat Rec 182:355–366
Taxi J (1979) The chromaffin and chromaffm-like cells in the autonomic nervous system. Int Rev Cytol 57:283–343
Ungváry G, Léránth C (1970) Termination in the prevertebral abdominal sympathetic ganglia of axons arising from the local (terminal) vegetative plexus of visceral organs. Z Zellforsch 110:185–191
Yanaihara N, Yanaihara C, Mochizuki T, Iwahara K, Fujita T, Iwanaga T (1981) Immunoreactive GRP. Peptides 2:185–191
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Macrae, I.M., Furness, J.B. & Costa, M. Distribution of subgroups of noradrenaline neurons in the coeliac ganglion of the guinea-pig. Cell Tissue Res. 244, 173–180 (1986). https://doi.org/10.1007/BF00218395
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218395