Abstract
The chemistry and the physics of voltage sensitive dyes (VSDs) should be understood and appreciated as a prerequisite for their optimal application to problems in neuroscience cardiology. This chapter provides a basic understanding of the properties of the large variety of available organic VSDs. The mechanisms by which the dyes respond to voltage guides the best set up of the optics for recording or imaging electrophysiological activity. The physical and chemical properties of the dyes can be tuned to optimize delivery to and staining of the cells in different experimental preparations. The aim of this chapter is to arm the experimentalists who use the dyes with enough information and data to be able to intelligently choose the best dye for their specific requirements.
Keywords
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Acknowledgment
I am indebted to the many talented chemists, microscopists and neuroscientists who have collaborated with me and who have carried out much of the research summarized in this chapter. Most notably, I wish to acknowledge my long term collaborators Larry Cohen, Aaron Lewis, Mei-de Wei and Joe Wuskell. The more recent work in my lab has benefited from collaborations with Ping Yan, Corey Acker and Srdjan Antic. This work was supported by NIH EB001963.
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Loew, L.M. (2015). Design and Use of Organic Voltage Sensitive Dyes. In: Canepari, M., Zecevic, D., Bernus, O. (eds) Membrane Potential Imaging in the Nervous System and Heart. Advances in Experimental Medicine and Biology, vol 859. Springer, Cham. https://doi.org/10.1007/978-3-319-17641-3_2
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