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Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics

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Book cover Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 806))

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein–protein interactions, and protein turnover.

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Authors and Affiliations

  1. Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 540 First Avenue, New York, NY, 10016, USA

    Esthelle Hoedt, Guoan Zhang & Thomas A. Neubert

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  1. Esthelle Hoedt
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  2. Guoan Zhang
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  3. Thomas A. Neubert
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Correspondence to Thomas A. Neubert .

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Editors and Affiliations

  1. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Alisa G. Woods

  2. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Costel C. Darie

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Hoedt, E., Zhang, G., Neubert, T.A. (2014). Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_5

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