Elsevier

Developmental Biology

Volume 208, Issue 1, 1 April 1999, Pages 166-188
Developmental Biology

Regular Article
Distinct Neural Stem Cells Proliferate in Response to EGF and FGF in the Developing Mouse Telencephalon

https://doi.org/10.1006/dbio.1998.9192Get rights and content
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Abstract

Multipotent, self-renewing neural stem cells reside in the embryonic mouse telencephalic germinal zone. Using anin vitroneurosphere assay for neural stem cell proliferation, we demonstrate that FGF-responsive neural stem cells are present as early as E8.5 in the anterior neural plate, but EGF-responsive neural stem cells emerge later in development in a temporally and spatially specific manner. By separately blocking EGF and FGF2 signaling, we also show that EGF alone and FGF2 alone can independently elicit neural stem cell proliferation and at relatively high cell densities separate cell nonautonomous effects can substantially enhance the mitogen-induced proliferation. At lower cell densities, neural stem cell proliferation is additive in the presence of EGF and FGF2 combined, revealing two different stem cell populations. However, both FGF-responsive and EGF-responsive neural stem cells retain their self-renewal and multilineage potential, regardless of growth factor conditions. These results support a model in which separate, lineage-related EGF- and FGF-responsive neural stem cells are present in the embryonic telencephalic germinal zone.

Keywords

EGF
FGF2
mouse
neural development
neural stem cells
proliferation
telencephalon

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