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Identification of a Novel Fibroblast Growth Factor, FGF-23, Preferentially Expressed in the Ventrolateral Thalamic Nucleus of the Brain☆

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Abstract

We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus (∼24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical (∼72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 (∼24% and ∼22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. Therefore, FGF-23 is expected a unique FGF that plays roles in the function of the ventrolateral thalamic nucleus.

References (25)

  • M. Yamasaki et al.

    J. Biol. Chem.

    (1996)
  • A. Miyake et al.

    Biochem. Biophys. Res. Commun.

    (1998)
  • M. Hoshikawa et al.

    Biochem. Biophys. Res. Commun.

    (1998)
  • N. Ohbayashi et al.

    J. Biol. Chem.

    (1998)
  • T. Nishimura et al.

    Biochim. Biophys. Acta

    (1999)
  • T. Nishimura et al.

    Biochim. Biophys. Acta

    (2000)
  • A. Baird et al.

    Cancer Cells

    (1991)
  • W.H. Burgess et al.

    Annu. Rev. Biochem.

    (1989)
  • A. Tanaka et al.

    Proc. Natl. Acad. Sci. USA

    (1992)
  • M. Miyamoto et al.

    Mol. Cell. Biol.

    (1993)
  • C. Dickson et al.

    Ann. NY Acad. Sci.

    (1991)
  • T. Yoshida et al.

    Ann. NY Acad. Sci.

    (1991)

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    ☆

    The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL, and GenBank nucleotide sequence databases with Accession Nos. AB037889 and AB037973.

    1

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