Statistics table
| Figures | Data structure | Type of test | Statistical information |
|---|---|---|---|
| Figure 1A | Normal distribution | One-way ANOVA | F(6,14) = 52.697, p < 0.0001 |
| Vehicle vs 0.01 μM | Dunnett's multiple comparisons test | CI: –1.8649 to –0.17292 | |
| Vehicle vs 0.1 μM | Dunnett's multiple comparisons test | CI: –3.6802 to –1.9882 | |
| Vehicle vs 1 μM | Dunnett's multiple comparisons test | CI: –3.9667 to –2.2748 | |
| Vehicle vs 10 μM | Dunnett's multiple comparisons test | CI: –3.5352 to –1.8432 | |
| Vehicle vs FAC | Dunnett's multiple comparisons test | CI: –4.198 to –2.5061 | |
| Vehicle vs DFO | Dunnett's multiple comparisons test | CI: –0.77749 to 0.91446 | |
| Figure 1B | Normal distribution | One-way ANOVA | F(3,8) = 6.2933, p = 0.0168 |
| Vehicle vs morphine | Dunnett's multiple comparisons test | CI: –1.9825 to –0.01226 | |
| Vehicle vs PTX | Dunnett's multiple comparisons test | CI: –1.0416 to 0.92856 | |
| Vehicle vs PTX + Mor | Dunnett's multiple comparisons test | CI: –0.53893 to 1.4313 | |
| Figure 1C | Normal distribution | One-way ANOVA | F(7,16) = 94.711, p < 0.0001 |
| Vehicle vs 30 m Mor | Dunnett's multiple comparisons test | CI: –0.49618 to 1.6318 | |
| Vehicle vs 6 h Mor | Dunnett's multiple comparisons test | CI: –1.2963 to 0.8317 | |
| Vehicle vs 24 h Mor | Dunnett's multiple comparisons test | CI: –1.6376 to 0.49035 | |
| Vehicle vs 24 h FAC | Dunnett's multiple comparisons test | CI: –6.7161 to –4.5881 | |
| Vehicle vs 1 h TNF | Dunnett's multiple comparisons test | CI: –2.109 to 0.019 | |
| Vehicle vs 3 h TNF | Dunnett's multiple comparisons test | CI: –6.4336 to –4.3057 | |
| Vehicle vs 24 h TNF | Dunnett's multiple comparisons test | CI: –0.98676 to 1.1412 | |
| Figure 1D | Normal distribution | One-way ANOVA | F(3,42) = 0.38357, p = 0.7654 |
| Vehicle vs 30 m Mor | Dunnett's multiple comparisons test | CI: –0.23702 to 0.11747 | |
| Vehicle vs 6 h Mor | Dunnett's multiple comparisons test | CI: –0.24894 to 0.10556 | |
| Vehicle vs 24 h Mor | Dunnett's multiple comparisons test | CI: –0.21279 to 0.14933 | |
| Figure 2B, cytoplasmic | Normal distribution | One-way ANOVA | F(3,8) = 24.28, p = 0.0002 |
| Vehicle vs 3 h Mor | Dunnett's multiple comparisons test | CI: –1.002 to 0.4371 | |
| Vehicle vs 6 h Mor | Dunnett's multiple comparisons test | CI: –1.629 to –0.1897 | |
| Vehicle vs 24 h Mor | Dunnett's multiple comparisons test | CI: –2.684 to –1.245 | |
| Figure 2B, nuclear | Normal distribution | One-way ANOVA | F(3,8) = 1.644, p = 0.2549 |
| Vehicle vs 3 h Mor | Dunnett's multiple comparisons test | CI: –0.6596 to 0.767 | |
| Vehicle vs 6 h Mor | Dunnett's multiple comparisons test | CI: –0.7541 to 0.6725 | |
| Vehicle vs 24 h Mor | Dunnett's multiple comparisons test | CI: –1.151 to 0.2751 | |
| Figure 3A, spine density | Normal distribution | Two-tailed, unpaired t test | t(16) = 9.372, CI: –2.825 to –1.783 |
| Figure 3A, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(3,64) = 13.9, p < 0.0001Treatment F(3,64) = 151.9, p < 0.0001Morphology F(1,64) = 81.83, p < 0.0001 |
| Vehicle, morphine | |||
| Thin | Sidak's multiple comparisons test | CI: 0.7779 to 1.428 | |
| Stubby | Sidak's multiple comparisons test | CI: 0.007506 to 0.6573 | |
| Mushroom | Sidak's multiple comparisons test | CI: 0.4853 to 1.135 | |
| Filopodia | Sidak's multiple comparisons test | CI: –0.2768 to 0.373 | |
| Figure 3B, spine density | Normal distribution | One-way ANOVA | F(4,40) = 50.32, p < 0.0001 |
| Vehicle vs 0.01 μM | Tukey's multiple comparisons test | CI: 0.0951 to 1.616 | |
| Vehicle vs 0.1 μM | Tukey's multiple comparisons test | CI: 1.048 to 2.569 | |
| Vehicle vs 1 μM | Tukey's multiple comparisons test | CI: 2.003 to 3.524 | |
| Vehicle vs 10 μM | Tukey's multiple comparisons test | CI: 2.487 to 4.008 | |
| 0.01 vs 0.1 μM | Tukey's multiple comparisons test | CI: 0.1923 to 1.713 | |
| 0.1 vs 1 μM | Tukey's multiple comparisons test | CI: 0.1951 to 1.716 | |
| 1 vs 10 μM | Tukey's multiple comparisons test | CI: –0.2771 to 1.244 | |
| Figure 3B, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(12,160) = 21.58, p < 0.0001Morphology F(3,160) = 956.9, p < 0.0001Treatment F(4,160) = 42.9, p < 0.0001 |
| Filopodia | |||
| Vehicle vs 0.01 μM | Tukey's multiple comparisons test | CI: –0.4073 to 0.3795 | |
| Vehicle vs 0.1 μM | Tukey's multiple comparisons test | CI: –0.1906 to 0.5962 | |
| Vehicle vs 1 μM | Tukey's multiple comparisons test | CI: –0.3156 to 0.4712 | |
| Vehicle vs 10 μM | Tukey's multiple comparisons test | CI: –0.2767 to 0.5101 | |
| Mushroom | |||
| Vehicle vs 0.01 μM | Tukey's multiple comparisons test | CI: –0.1934 to 0.5934 | |
| Vehicle vs 0.1 μM | Tukey's multiple comparisons test | CI: 0.006604 to 0.7934 | |
| Vehicle vs 1 μM | Tukey's multiple comparisons test | CI: 0.08438 to 0.8712 | |
| Vehicle vs 10 μM | Tukey's multiple comparisons test | CI: 0.1983 to 0.9851 | |
| Stubby | |||
| Vehicle vs 0.01 μM | Tukey's multiple comparisons test | CI: –0.3934 to 0.3934 | |
| Vehicle vs 0.1 μM | Tukey's multiple comparisons test | CI: –0.4378 to 0.349 | |
| Vehicle vs 1 μM | Tukey's multiple comparisons test | CI: –0.3434 to 0.4434 | |
| Vehicle vs 10 μM | Tukey's multiple comparisons test | CI: –0.3295 to 0.4573 | |
| Thin | |||
| Vehicle vs 0.01 μM | Tukey's multiple comparisons test | CI: 0.4372 to 1.224 | |
| Vehicle vs 0.1 μM | Tukey's multiple comparisons test | CI: 0.803 to 1.59 | |
| Vehicle vs 1 μM | Tukey's multiple comparisons test | CI: 1.77 to 2.557 | |
| Vehicle vs 10 μM | Tukey's multiple comparisons test | CI: 2.114 to 2.9 | |
| 0.01 vs 0.1 μM | Tukey's multiple comparisons test | CI: –0.02756 to 0.7592 | |
| 0.1 vs 1 μM | Tukey's multiple comparisons test | CI: 0.5741 to 1.361 | |
| 1 vs 10 μM | Tukey's multiple comparisons test | CI: –0.05034 to 0.7365 | |
| Figure 3C, spine density | Normal distribution | One-way ANOVA | F(5,42) = 15.29, p < 0.0001 |
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 1.608 to 3.41 | |
| Vehicle vs PTX | Tukey's multiple comparisons test | CI: 0.0146 to 1.817 | |
| Vehicle vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.3166 to 1.485 | |
| Vehicle vs CTAP | Tukey's multiple comparisons test | CI: 0.2709 to 2.073 | |
| Vehicle vs CTAP + Mor | Tukey's multiple comparisons test | CI: 0.09273 to 1.895 | |
| PTX vs PTX + Mor | Tukey's multiple comparisons test | CI: –1.232 to 0.5698 | |
| CTAP vs CTAP + Mor | Tukey's multiple comparisons test | CI: –1.079 to 0.7229 | |
| Figure 3C, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(15,168) = 9.193, p < 0.0001Morphology F(3,168) = 1448, p < 0.0001Treatment F(5,168) = 17.39, p < 0.0001 |
| Filopodia | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: –0.3425 to 0.4612 | |
| Vehicle vs PTX | Tukey's multiple comparisons test | CI: –0.3894 to 0.4144 | |
| Vehicle vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.4519 to 0.3519 | |
| Vehicle vs CTAP | Tukey's multiple comparisons test | CI: –0.3675 to 0.4362 | |
| Vehicle vs CTAP + Mor | Tukey's multiple comparisons test | CI: –0.3487 to 0.455 | |
| Mushroom | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 0.1544 to 0.9581 | |
| Vehicle vs PTX | Tukey's multiple comparisons test | CI: –0.2019 to 0.6019 | |
| Vehicle vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.2956 to 0.5081 | |
| Vehicle vs CTAP | Tukey's multiple comparisons test | CI: –0.08937 to 0.7144 | |
| Vehicle vs CTAP + Mor | Tukey's multiple comparisons test | CI: –0.1081 to 0.6956 | |
| PTX vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.4956 to 0.3081 | |
| CTAP vs CTAP + Mor | Tukey's multiple comparisons test | CI: –0.4206 to 0.3831 | |
| Stubby | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: –0.4644 to 0.3394 | |
| Vehicle vs PTX | Tukey's multiple comparisons test | CI: –0.5612 to 0.2425 | |
| Vehicle vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.4206 to 0.3831 | |
| Vehicle vs CTAP | Tukey's multiple comparisons test | CI: –0.4144 to 0.3894 | |
| Vehicle vs CTAP + Mor | Tukey's multiple comparisons test | CI: –0.38 to 0.4237 | |
| Thin | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 1.404 to 2.208 | |
| Vehicle vs PTX | Tukey's multiple comparisons test | CI: 0.1481 to 0.9519 | |
| Vehicle vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.03 to 0.7737 | |
| Vehicle vs CTAP | Tukey's multiple comparisons test | CI: 0.4544 to 1.258 | |
| Vehicle vs CTAP + Mor | Tukey's multiple comparisons test | CI: –0.03625 to 0.7675 | |
| PTX vs PTX + Mor | Tukey's multiple comparisons test | CI: –0.58 to 0.2237 | |
| CTAP vs CTAP + Mor | Tukey's multiple comparisons test | CI: –0.8925 to –0.08875 | |
| Figure 4A | Normal distribution | Two-tailed, unpaired t test | t(6) = 2.717, CI: 0.6332 to 12.11 |
| Figure 4B, spine density | Normal distribution | Two-tailed, unpaired t test | t(10) = 8.482, CI: –7.026 to –4.103 |
| Figure 4B, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(3,40) = 7.579, p = 0.0004Morphology F(3,40) = 114, p < 0.0001Treatment F(1,40) = 44.5, p < 0.0001 |
| Vehicle, morphine | |||
| Thin | Sidak's multiple comparisons test | CI: 1.922 to 4.195 | |
| Stubby | Sidak's multiple comparisons test | CI: –0.2348 to 2.038 | |
| Mushroom | Sidak's multiple comparisons test | CI: 0.4543 to 2.727 | |
| Filopodia | Sidak's multiple comparisons test | CI: –0.8738 to 1.399 | |
| Figure 5A | Normal distribution | Two-way ANOVA | Interaction F(4,20) = 0.1919, p = 0.9398Treatment F(4,20) = 12.94, p < 0.0001Expression F(1,20) = 0.002895, p = 0.9576 |
| Vehicle:FHC vs 30 m FAC:FHC | Tukey's multiple comparisons test | CI: –1.704 to 1.063 | |
| Vehicle:FHC vs 6 h FAC:FHC | Tukey's multiple comparisons test | CI: –1.8 to 0.9668 | |
| Vehicle:FHC vs 24 h FAC:FHC | Tukey's multiple comparisons test | CI: –2.94 to –0.1729 | |
| Vehicle:FHC vs DFO:FHC | Tukey's multiple comparisons test | CI: –1.299 to 1.468 | |
| Vehicle:FLC vs 30 m FAC:FLC | Tukey's multiple comparisons test | CI: –1.433 to 1.334 | |
| Vehicle:FLC vs 6 h FAC:FLC | Tukey's multiple comparisons test | CI: –1.762 to 1.005 | |
| Vehicle:FLC vs 24 h FAC:FLC | Tukey's multiple comparisons test | CI: –3.136 to –0.3684 | |
| Vehicle:FLC vs DFO:FLC | Tukey's multiple comparisons test | CI: –1.365 to 1.402 | |
| 24 h FAC:FHC vs 24 h FAC:FLC | Tukey's multiple comparisons test | CI: –1.579 to 1.188 | |
| Figure 5B | Normal distribution | Two-way ANOVA | Interaction F(3,24) = 1.244, p = 0.3157Treatment F(3,24) = 22.94, p < 0.0001Expression F(1,24) = 9.252, p = 0.0056 |
| Vehicle:FHC vs 30 m Mor:FHC | Sidak's multiple comparisons test | CI: –1.581 to 0.5878 | |
| Vehicle:FHC vs 6 h Mor:FHC | Sidak's multiple comparisons test | CI: –2.202 to –0.03317 | |
| Vehicle:FHC vs 24 h Mor:FHC | Sidak's multiple comparisons test | CI: –3.17 to –1.001 | |
| Vehicle:FLC vs 30 m Mor:FLC | Sidak's multiple comparisons test | CI: –1.118 to 1.051 | |
| Vehicle:FLC vs 6 h Mor:FLC | Sidak's multiple comparisons test | CI: –1.596 to 0.5731 | |
| Vehicle:FLC vs 24 h Mor:FLC | Sidak's multiple comparisons test | CI: –2.356 to –0.1869 | |
| 30 m Mor:FHC vs 30 m Mor:FLC | Sidak's multiple comparisons test | CI: –0.6212 to 1.548 | |
| 6 h Mor:FHC vs 6 h Mor:FLC | Sidak's multiple comparisons test | CI: –0.4783 to 1.691 | |
| 24 h Mor:FHC vs 24 h Mor:FLC | Sidak's multiple comparisons test | CI: –0.2706 to 1.899 | |
| Figure 5C | Normal distribution | Two-way ANOVA | Interaction F(1,12) = 3.823, p = 0.0742Treatment F(1,12) = 43.94, p < 0.0001Expression F(1,12) = 3.814, p = 0.0745 |
| Vehicle:FHC vs morphine:FHC | Tukey's multiple comparisons test | CI: –1.358 to –0.4659 | |
| Vehicle:FLC vs morphine:FHC | Tukey's multiple comparisons test | CI: –1.358 to –0.4657 | |
| Morphine:FHC vs morphine:FLC | Tukey's multiple comparisons test | CI: –0.03085 to 0.8613 | |
| Figure 6B | Normal distribution | One-way ANOVA | F(4,8) = 112, p < 0.0001 |
| Vehicle vs 0.1 μM | Dunnett's multiple comparisons test | CI: –1.472 to 2.722 | |
| Vehicle vs 1 μM | Dunnett's multiple comparisons test | CI: 1.293 to 5.487 | |
| Vehicle vs 10 μM | Dunnett's multiple comparisons test | CI: 8.804 to 13.49 | |
| Vehicle vs 100 μM | Dunnett's multiple comparisons test | CI: 10.07 to 14.76 | |
| Figure 6D | Normal distribution | One-way ANOVA | F(4,13) = 47.98, p < 0.0001 |
| Vehicle vs 0.1 μM | Dunnett's multiple comparisons test | CI: –26.25 to 4.68 | |
| Vehicle vs 1 μM | Dunnett's multiple comparisons test | CI: –34.28 to –6.624 | |
| Vehicle vs 10 μM | Dunnett's multiple comparisons test | CI: –60.4 to –35.66 | |
| Vehicle vs 100 μM | Dunnett's multiple comparisons test | CI: –61.63 to –36.89 | |
| Figure 6E, top | Normal distribution | One-way ANOVA | F(3,8) = 180.2, p < 0.0001 |
| Vehicle vs 0.1 μM | Dunnett's multiple comparisons test | CI: –0.2068 to 0.0702 | |
| Vehicle vs 1 μM | Dunnett's multiple comparisons test | CI: –0.6368 to –0.3598 | |
| Vehicle vs 10 μM | Dunnett's multiple comparisons test | CI: –1.127 to –0.8498 | |
| Figure 6E, bottom | Normal distribution | One-way ANOVA | F(3,8) = 127.2, p < 0.0001 |
| Vehicle vs morphine | Dunnett's multiple comparisons test | CI: –1.17 to –0.8066 | |
| Vehicle vs naloxone | Dunnett's multiple comparisons test | CI: –0.11 to 0.2534 | |
| Vehicle vs Nal + Mor | Dunnett's multiple comparisons test | CI: –0.21 to 0.1534 | |
| Figure 6G, EL | Normal distribution | Two-tailed, unpaired t test | t(4) = 7.036, CI: –27.35 to –11.87 |
| Figure 6G, cytosol | Normal distribution | Two-tailed, unpaired t test | t(4) = 16.86, CI: 11.13 to 15.52 |
| Figure 6H, EL | Normal distribution | One-way ANOVA | F(3,8) = 541.2, p < 0.0001 |
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 30.67 to 36.88 | |
| Vehicle vs naloxone | Tukey's multiple comparisons test | CI: –2.663 to 3.55 | |
| Vehicle vs Nal + Mor | Tukey's multiple comparisons test | CI: 4.1 to 10.31 | |
| Morphine vs Nal + Mor | Tukey's multiple comparisons test | CI: –29.68 to –23.46 | |
| Figure 6H, cytosol | Normal distribution | One-way ANOVA | F(3,8) = 26.27, p = 0.0002 |
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: –14.9 to –5.351 | |
| Vehicle vs DFO | Tukey's multiple comparisons test | CI: –4.623 to 4.929 | |
| Vehicle vs DFO + Mor | Tukey's multiple comparisons test | CI: –3.169 to 6.383 | |
| Morphine vs DFO + Mor | Tukey's multiple comparisons test | CI: 6.957 to 16.51 | |
| Figure 6I | Normal distribution | One-way ANOVA | F(3,8) = 45.05, p < 0.0001 |
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: –0.249 to –0.131 | |
| Vehicle vs naloxone | Tukey's multiple comparisons test | CI: –0.06895 to 0.04895 | |
| Vehicle vs Nal + Mor | Tukey's multiple comparisons test | CI: –0.119 to –0.001048 | |
| Morphine vs Nal + Mor | Tukey's multiple comparisons test | CI: 0.07105 to 0.189 | |
| Figure 7A | Normal distribution | One-way ANOVA | F(7,16) = 87.91, p < 0.0001 |
| Vehicle vs 30 m Mor | Dunnett's multiple comparisons test | CI: –678.2 to –19.94 | |
| Vehicle vs 3 h Mor | Dunnett's multiple comparisons test | CI: –1010 to –351.8 | |
| Vehicle vs 6 h Mor | Dunnett's multiple comparisons test | CI: –1033 to –374.9 | |
| Vehicle vs 24 h Mor | Dunnett's multiple comparisons test | CI: –741.5 to –83.21 | |
| Vehicle vs Phen | Dunnett's multiple comparisons test | CI: –27.91 to 630.4 | |
| Vehicle vs Phen + 24 h Mor | Dunnett's multiple comparisons test | CI: –208.3 to 449.9 | |
| Vehicle vs FAC | Dunnett's multiple comparisons test | CI: –2417 to –1759 | |
| Figure 7B | Normal distribution | One-way ANOVA | F(5,14) = 13.72, p < 0.0001 |
| Vehicle vs morphine | Dunnett's multiple comparisons test | CI: –0.954 to –0.06 | |
| Vehicle vs DFO | Dunnett's multiple comparisons test | CI: –0.06606 to 0.8279 | |
| Vehicle vs Mor + DFO | Dunnett's multiple comparisons test | CI: –0.009428 to 0.8182 | |
| Vehicle vs DTPA | Dunnett's multiple comparisons test | CI: –0.1879 to 0.7061 | |
| Vehicle vs Mor + DTPA | Dunnett's multiple comparisons test | CI: –0.9824 to –0.08839 | |
| Figure 7C, spine density | Normal distribution | One-way ANOVA | F(6,56) = 24.21, p < 0.0001 |
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 1.869 to 3.642 | |
| Vehicle vs FAC | Tukey's multiple comparisons test | CI: 1.494 to 3.267 | |
| Vehicle vs DFO | Tukey's multiple comparisons test | CI: 0.1525 to 1.925 | |
| Vehicle vs DFO + Mor | Tukey's multiple comparisons test | CI: 0.1108 to 1.884 | |
| Vehicle vs DTPA | Tukey's multiple comparisons test | CI: –0.3058 to 1.467 | |
| Vehicle vs DTPA + Mor | Tukey's multiple comparisons test | CI: 1.13 to 2.903 | |
| Morphine vs FAC | Tukey's multiple comparisons test | CI: –1.261 to 0.5114 | |
| DFO vs DFO + Mor | Tukey's multiple comparisons test | CI: –0.928 to 0.8447 | |
| DTPA vs DTPA + Mor | Tukey's multiple comparisons test | CI: 0.5497 to 2.322 | |
| Figure 7C, spine morphology | Normal distribution | Two-way ANOVA | Interaction F(18,224) = 17.58Morphology F(3,224) = 1991, p < 0.0001Treatment F(6,224) = 31.61, p < 0.0001 |
| Filopodia | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: –0.254 to 0.504 | |
| Vehicle vs FAC | Tukey's multiple comparisons test | CI: –0.2623 to 0.4956 | |
| Vehicle vs DFO | Tukey's multiple comparisons test | CI: –0.2984 to 0.4595 | |
| Vehicle vs DFO + Mor | Tukey's multiple comparisons test | CI: –0.3355 to 0.4225 | |
| Vehicle vs DTPA | Tukey's multiple comparisons test | CI: –0.2568 to 0.5012 | |
| Vehicle vs DTPA + Mor | Tukey's multiple comparisons test | CI: –0.2512 to 0.5068 | |
| Mushroom | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 0.1044 to 0.8623 | |
| Vehicle vs FAC | Tukey's multiple comparisons test | CI: 0.07103 to 0.829 | |
| Vehicle vs DFO | Tukey's multiple comparisons test | CI: –0.1206 to 0.6373 | |
| Vehicle vs DFO + Mor | Tukey's multiple comparisons test | CI: –0.05953 to 0.6984 | |
| Vehicle vs DTPA | Tukey's multiple comparisons test | CI: –0.2068 to 0.5512 | |
| Vehicle vs DTPA + Mor | Tukey's multiple comparisons test | CI: 0.03769 to 0.7956 | |
| Morphine vs FAC | Tukey's multiple comparisons test | CI: –0.4123 to 0.3456 | |
| DFO vs DFO + Mor | Tukey's multiple comparisons test | CI: –0.3179 to 0.4401 | |
| DTPA vs DTPA + Mor | Tukey's multiple comparisons test | CI: –0.1345 to 0.6234 | |
| Stubby | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: –0.3845 to 0.3734 | |
| Vehicle vs FAC | Tukey's multiple comparisons test | CI: –0.3706 to 0.3873 | |
| Vehicle vs DFO | Tukey's multiple comparisons test | CI: –0.4429 to 0.3151 | |
| Vehicle vs DFO + Mor | Tukey's multiple comparisons test | CI: –0.3956 to 0.3623 | |
| Vehicle vs DTPA | Tukey's multiple comparisons test | CI: –0.3956 to 0.3623 | |
| Vehicle vs DTPA + Mor | Tukey's multiple comparisons test | CI: –0.3929 to 0.3651 | |
| Thin | |||
| Vehicle vs morphine | Tukey's multiple comparisons test | CI: 1.788 to 2.546 | |
| Vehicle vs FAC | Tukey's multiple comparisons test | CI: 1.457 to 2.215 | |
| Vehicle vs DFO | Tukey's multiple comparisons test | CI: 0.4081 to 1.166 | |
| Vehicle vs DFO + Mor | Tukey's multiple comparisons test | CI: 0.3432 to 1.101 | |
| Vehicle vs DTPA | Tukey's multiple comparisons test | CI: –0.07897 to 0.679 | |
| Vehicle vs DTPA + Mor | Tukey's multiple comparisons test | CI: 1.121 to 1.879 | |
| Morphine vs FAC | Tukey's multiple comparisons test | CI: –0.7095 to 0.04842 | |
| DFO vs DFO + Mor | Tukey's multiple comparisons test | CI: –0.4438 to 0.3142 | |
| DTPA vs DTPA + Mor | Tukey's multiple comparisons test | CI: 0.821 to 1.579 | |