Table 4.

Detailed results and statistical analyses related to developmental milestones

Weight
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		466.906	0.000	1.000	−	−	−
Day × genotype effect		2.275	0.045	0.754	−	−	−
Day × gender effect		0.363	0.765	0.117	−	−	−
Day × genotype × gender effect		0.569	0.742	0.214	−	−	−
Genotype effect		3.046	0.048	0.560	0.144	0.018	0.147
Gender effect		0.933	0.339	0.157	−	−	−
Genotype × gender		0.686	0.509	0.158	−	−	−

											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Weight - P1	Nonnormal	1.47 ± 0.02	1.38 ± 0.02	1.36 ± 0.03	2.244	0.118	0.433	−	−	−	1.067	0.307	0.173	0.016	0.984	0.052
Weight - P2	Nonnormal	1.51 ± 0.03	1.42 ± 0.03	1.4 ± 0.04	2.010	0.146	0.393	−	−	−	0.510	0.479	0.108	0.193	0.825	0.078
Weight - P3	Nonnormal	1.62 ± 0.05	1.59 ± 0.04	1.55 ± 0.07	0.451	0.640	0.119	−	−	−	0.030	0.863	0.053	1.047	0.360	0.221
Weight - P4	Nonnormal	1.95 ± 0.09	1.87 ± 0.06	1.87 ± 0.1	0.610	0.548	0.145	−	−	−	0.822	0.369	0.144	0.378	0.688	0.107
Weight - P5	Nonnormal	2.34 ± 0.1	2.27 ± 0.07	2.27 ± 0.12	0.305	0.739	0.095	−	−	−	0.803	0.375	0.142	1.021	0.368	0.217
Weight - P6	Nonnormal	2.77 ± 0.14	2.71 ± 0.08	2.7 ± 0.15	0.320	0.728	0.098	−	−	−	0.436	0.512	0.099	0.356	0.703	0.104
Weight - P7	Nonnormal	3.29 ± 0.12	3.25 ± 0.09	3.13 ± 0.15	0.682	0.511	0.158	−	−	−	0.835	0.366	0.145	0.934	0.401	0.201
Weight - P8	Nonnormal	3.8 ± 0.14	3.73 ± 0.1	3.65 ± 0.15	0.493	0.614	0.126	−	−	−	0.723	0.400	0.132	1.023	0.368	0.217
Weight - P9	Nonnormal	4.26 ± 0.14	4.23 ± 0.1	4 ± 0.17	1.146	0.327	0.239	−	−	−	3.146	0.083	0.411	0.883	0.421	0.192
Weight - P10	Nonnormal	4.86 ± 0.11	4.72 ± 0.1	4.58 ± 0.16	1.013	0.371	0.215	−	−	−	0.299	0.587	0.083	0.051	0.951	0.057
Weight - P11	Nonnormal	5.42 ± 0.11	5.21 ± 0.1	5.03 ± 0.18	1.837	0.171	0.363	−	−	−	0.781	0.382	0.139	0.023	0.978	0.053
Weight - P12	Nonnormal	5.85 ± 0.11	5.7 ± 0.11	5.39 ± 0.13	2.148	0.129	0.417	−	−	−	0.092	0.764	0.060	0.362	0.698	0.105
Weight - P13	Nonnormal	6.22 ± 0.12	6.01 ± 0.11	5.72 ± 0.2	1.787	0.179	0.354	−	−	−	0.853	0.361	0.147	0.657	0.524	0.153
Weight - P14	Nonnormal	6.62 ± 0.12	6.42 ± 0.11	5.83 ± 0.17	4.891	0.012	0.777	0.274	0.004	0.016	0.577	0.451	0.115	0.618	0.544	0.147
Weight - P15	Nonnormal	7.01 ± 0.14	6.73 ± 0.12	6.38 ± 0.22	2.504	0.093	0.476	0.175	0.031	0.198	0.595	0.445	0.117	0.238	0.789	0.085
Weight - P16	Nonnormal	7.31 ± 0.14	6.96 ± 0.13	6.69 ± 0.19	2.668	0.081	0.502	0.094	0.030	0.318	0.157	0.694	0.067	0.072	0.931	0.060
Weight - P17	Nonnormal	7.55 ± 0.14	7.2 ± 0.13	6.83 ± 0.22	2.973	0.061	0.549	0.118	0.020	0.192	0.889	0.351	0.152	0.170	0.845	0.075
Weight - P18	Nonnormal	7.76 ± 0.14	7.43 ± 0.14	6.98 ± 0.2	3.160	0.050	0.577	0.152	0.016	0.127	0.790	0.379	0.140	0.187	0.830	0.077
Weight - P19	Nonnormal	7.98 ± 0.13	7.58 ± 0.16	7.1 ± 0.18	3.534	0.038	0.628	0.115	0.011	0.121	1.170	0.285	0.185	0.861	0.430	0.189
Weight - P20	Nonnormal	8.31 ± 0.19	7.69 ± 0.19	7.18 ± 0.2	4.268	0.020	0.716	0.051	0.006	0.146	0.729	0.398	0.133	1.415	0.254	0.287
Weight - P21	Nonnormal	8.67 ± 0.21	8.05 ± 0.27	7.38 ± 0.28	3.366	0.044	0.605	0.127	0.013	0.127	0.263	0.611	0.079	0.839	0.439	0.185

Eye opening
Repeated measures, sphericity assumed		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		192.080	0.000	1.000	−	−	−
Day × genotype effect		1.565	0.190	0.469	−	−	−
Day × gender effect		0.716	0.494	0.169	−	−	−
Day × genotype × gender effect		0.653	0.629	0.544
Genotype effect		1.403	0.257	0.285	−	−	−
Gender effect		1.852	0.181	0.265	−	−	−
Genotype × gender		0.957	0.392	0.205
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Eye opening score - P9	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Eye opening score - P10	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Eye opening score - P11	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Eye opening score - P12	Nonnormal	0.3 ± 0.2	0.28 ± 0.13	0.1 ± 0.1	0.534	0.590	0.132	−	−	−	1.917	0.173	0.273	0.496	0.613	0.126
Eye opening score - P13	Nonnormal	1.23 ± 0.34	1.35 ± 0.25	0.6 ± 0.3	1.445	0.247	0.292	−	−	−	0.707	0.405	0.130	0.032	0.969	0.055
Eye opening score - P14	Nonnormal	2.38 ± 0.18	2.75 ± 0.16	2.1 ± 0.09	4.723	0.014	0.761	0.134	0.167	0.005	2.464	0.124	0.336	2.248	0.118	0.433
Eye opening score - P15	Nonnormal	3 ± 0.25	3.1 ± 0.17	2.7 ± 0.26	0.646	0.529	0.151	−	−	−	0.043	0.837	0.055	1.262	0.293	0.260
Eye opening score - P16	Nonnormal	4 ± 0	3.85 ± 0.06	3.9 ± 0.1	0.734	0.486	0.166	−	−	−	3.076	0.087	0.403	1.249	0.297	0.257
Eye opening score - P17	Nonnormal	4 ± 0	3.85 ± 0.06	4 ± 0	1.665	0.201	0.332	−	−	−	1.155	0.288	0.183	1.971	0.152	0.386
Eye opening score - P18	Nonnormal	4 ± 0	3.89 ± 0.05	4 ± 0	0.957	0.392	0.205	−	−	−	0.690	0.411	0.128	1.041	0.362	0.220
Eye opening score - P19	Nonnormal	4 ± 0	3.96 ± 0.03	4 ± 0	0.428	0.654	0.115	−	−	−	0.320	0.575	0.086	0.426	0.656	0.115
Eye opening score - P20	−	4 ± 0	4 ± 0	4 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Average day of full opening	Nonnormal	15.53 ± 0.18	15.57 ± 0.33	15.9 ± 0.17	0.469	0.629	0.122	−	−	−	1.472	0.232	0.220	0.749	0.479	0.169

Ear opening
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		316.707	0.000	1.000	−	−	−
Day × genotype effect		0.807	0.594	0.361	−	−	−
Day × gender effect		2.150	0.079	0.617	−	−	−
Day × genotype × gender effect		1.056	0.396	0.472	−	−	−
Genotype effect		0.113	0.893	0.066	−	−	−
Gender effect		0.438	0.512	0.099	−	−	−
Genotype × gender		0.676	0.514	0.156	−	−	−

											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Ear opening score - P1	Nonnormal	0.23 ± 0.23	0.13 ± 0.09	0 ± 0	0.753	0.477	0.170	−	−	−	2.371	0.131	0.325	0.669	0.517	0.155
Ear opening score - P2	Nonnormal	2.15 ± 0.15	2.06 ± 0.04	2 ± 0	0.675	0.514	0.156	−	−	−	2.261	0.140	0.313	0.468	0.629	0.122
Ear opening score - P3	Nonnormal	2.38 ± 0.21	2.31 ± 0.13	2.3 ± 0.21	0.123	0.885	0.068	−	−	−	1.054	0.310	0.171	0.994	0.378	0.212
Ear opening score - P4	Nonnormal	3.15 ± 0.27	3.27 ± 0.13	3.6 ± 0.22	0.966	0.389	0.207	−	−	−	2.693	0.108	0.362	1.501	0.234	0.303
Ear opening score - P5	Nonnormal	4.15 ± 0.1	4.2 ± 0.11	4.1 ± 0.1	0.167	0.847	0.074	−	−	−	0.106	0.746	0.062	0.382	0.685	0.108
Ear opening score - P6	Nonnormal	5.76 ± 0.16	5.93 ± 0.06	6 ± 0	1.052	0.358	0.222	−	−	−	0.780	0.382	0.139	0.733	0.486	0.166
Ear opening score - P7	−	6 ± 0	6 ± 0	6 ± 0	0.439	0.647	0.117	−	−	−	0.270	0.606	0.080	0.617	0.544	0.146
Ear opening score - P8	−	6 ± 0	6 ± 0	6 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Ear opening score - P9	−	6 ± 0	6 ± 0	6 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Average day of full opening	Nonnormal	6.15 ± 0.1	5.93 ± 0.06	6 ± 0	0.622	0.541	0.147	−	−	−	0.070	0.793	0.058	0.274	0.761	0.091

Tooth eruption
Bottom incisor - repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		120.634	0.000	1.000	−	−	−
Day × genotype effect		1.452	0.177	0.648	−	−	−
Day × gender effect		1.873	0.116	0.564	−	−	−
Day × genotype × gender effect		1.671	0.107	0.723	−	−	−
Genotype effect		1.855	0.169	0.366	−	−	−
Gender effect		0.094	0.761	0.060	−	−	−
Genotype × gender		0.637	0.533	0.150	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Bottom incisor score - P7	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Bottom incisor score - P8	Nonnormal	0.38 ± 0.14	0.06 ± 0.04	0.1 ± 0.1	3.701	0.033	0.650	0.010	0.072	0.746	0.685	0.412	0.128	1.274	0.290	0.262
Bottom incisor score - P9	Nonnormal	0.92 ± 0.07	0.75 ± 0.08	1 ± 0.14	1.247	0.297	0.257	−	−	−	1.198	0.280	0.188	1.949	0.155	0.382
Bottom incisor score - P10	Nonnormal	1.15 ± 0.1	1.03 ± 0.06	1.1 ± 0.1	0.661	0.521	0.164	−	−	−	2.184	0.147	0.304	1.371	0.265	0.279
Bottom incisor score - P11	Nonnormal	1.84 ± 0.1	1.65 ± 0.08	1.5 ± 0.16	1.438	0.248	0.291	−	−	−	0.212	0.647	0.074	0.873	0.425	0.191
Bottom incisor score - P12	Nonnormal	1.92 ± 0.07	1.93 ± 0.04	1.8 ± 0.13	0.795	0.458	0.177	−	−	−	4.249	0.045	0.523	1.594	0.215	0.319
Bottom incisor score - P13	−	2 ± 0	2 ± 0	2 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Bottom incisor, day of full eruption	Nonnormal	11.07 ± 0.21	11.37 ± 0.15	11.5 ± 0.37	0.720	0.492	0.164	−	−	−	0.018	0.895	0.052	0.141	0.869	0.070

Top incisor - repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		41.000	0.000	1.000	−	−	−
Day × genotype effect		84.000	0.587	0.355	−	−	−
Day × gender effect		41.000	0.150	0.497	−	−	−
Day × genotype × gender effect		84.000	0.563	0.370	−	−	−
Genotype effect		0.314	0.732	0.097	−	−	−
Gender effect		0.845	0.363	0.147	−	−	−
Genotype × gender		1.028	0.366	0.218	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Top incisor score - P10	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Top incisor score - P11	Nonnormal	0.23 ± 0.12	0.1 ± 0.05	0 ± 0	1.757	0.184	0.348	−	−	−	0.021	0.886	0.052	0.903	0.413	0.196
Top incisor score - P12	Nonnormal	0.76 ± 0.12	0.79 ± 0.07	0.8 ± 0.13	0.010	0.990	0.051	−	−	−	1.285	0.263	0.198	1.558	0.222	0.313
Top incisor score - P13	Nonnormal	1.3 ± 0.17	1.24 ± 0.13	1 ± 0.21	0.590	0.559	0.142	−	−	−	0.009	0.925	0.051	0.804	0.454	0.179
Top incisor score - P14	Nonnormal	1.76 ± 0.12	1.79 ± 0.07	1.8 ± 0.13	0.010	0.990	0.051	−	−	−	1.285	0.263	0.198	1.558	0.222	0.313
Top incisor score - P15	Nonnormal	1.92 ± 0.07	1.89 ± 0.05	1.8 ± 0.13	0.487	0.618	0.125	−	−	−	4.489	0.040	0.545	0.861	0.430	0.189
Top incisor score - P16	−	2 ± 0	2 ± 0	2 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Top incisor, day of full eruption	Nonnormal	13.92 ± 0.26	14.41 ± 0.17	14.2 ± 0.32	1.110	0.339	0.233	−	−	−	0.816	0.371	0.143	1.006	0.374	0.214

Fur development
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		347.979	0.000	1.000	−	−	−
Day × genotype effect		0.885	0.546	0.458	−	−	−
Day × gender effect		1.948	0.089	0.646	−	−	−
Day × genotype × gender effect		3.234	0.001	0.986	−	−	−
Genotype effect		1.683	0.198	0.335	−	−	−
Gender effect		0.635	0.430	0.122	−	−	−
Genotype × gender		8.265	0.001	0.950	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Fur score - P1	Nonnormal	1 ± 0	0.92 ± 0.07	1 ± 0	0.439	0.647	0.117	−	−	−	0.270	0.606	0.080	0.617	0.544	0.146
Fur score - P2	Nonnormal	1.76 ± 0.12	1.79 ± 0.07	2 ± 0	1.484	0.238	0.300	−	−	−	0.000	0.995	0.050	6.724	0.003	0.897
Fur score - P3	Nonnormal	2.53 ± 0.18	2.37 ± 0.1	2.5 ± 0.16	0.898	0.415	0.195	−	−	−	1.736	0.194	0.252	7.221	0.002	0.918
Fur score - P4	Nonnormal	3.23 ± 0.2	3.13 ± 0.11	3.4 ± 0.16	1.605	0.212	0.321	−	−	−	5.973	0.019	0.667	9.904	0.000	0.978

Fur score - P5	Nonnormal	3.92 ± 0.13	3.86 ± 0.06	4 ± 0	1.034	0.364	0.219	−	−	−	0.013	0.909	0.051	4.885	0.012	0.777
Fur score - P6	Nonnormal	3.92 ± 0.13	3.89 ± 0.05	4 ± 0	0.657	0.523	0.153	−	−	−	0.090	0.766	0.060	4.026	0.025	0.689
Fur score - P7	Nonnormal	4.15 ± 0.19	4.27 ± 0.1	4.5 ± 0.16	1.002	0.375	0.213	−	−	−	0.353	0.556	0.089	1.378	0.263	0.281
Fur score - P8	Nonnormal	4.69 ± 0.17	4.62 ± 0.09	5 ± 0	2.746	0.075	0.514	−	−	−	1.116	0.297	0.178	4.075	0.024	0.694
Fur score - P9	Nonnormal	5 ± 0	4.93 ± 0.04	5 ± 0	0.927	0.403	0.200	−	−	−	0.604	0.441	0.118	1.203	0.310	0.249
Fur score - P10	Nonnormal	5.23 ± 0.12	5.24 ± 0.08	5.3 ± 0.15	0.125	0.882	0.068	−	−	−	0.007	0.936	0.051	2.343	0.108	0.450
Fur score - P11	Nonnormal	5.53 ± 0.14	5.72 ± 0.08	5.8 ± 0.13	0.906	0.411	0.196	−	−	−	0.997	0.324	0.164	3.754	0.031	0.656
Fur score - P12	Nonnormal	6 ± 0	5.96 ± 0.03	6 ± 0	0.401	0.672	0.111	−	−	−	0.274	0.603	0.081	0.479	0.622	0.123
Fur score - P13	−	6 ± 0	6 ± 0	6 ± 0	−	−	.	−	−	−	−	−	−	−	−	−
Fur score - P14	−	6 ± 0	6 ± 0	6 ± 0	−	−	.	−	−	−	−	−	−	−	−	−
Day of full fur	Nonnormal	11.3 ± 0.2	10.75 ± 0.37	10.9 ± 0.23	0.460	0.634	0.120	−	−	−	0.110	0.741	0.062	1.960	0.153	0.384

Auditory startle
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		56.506	0.000		−	−	−
Day × genotype effect		3.280	0.002		−	−	−
Day × gender effect		0.283	0.873		−	−	−
Day × genotype × gender effect		1.321	0.241		−	−	−
Genotype effect		12.867	0.000		0.070	0.000	0.000
Gender effect		0.058	0.811		−	−	−
Genotype × gender		0.358	0.701		−	−	−
											Gender effect			Gender × genotypeeffect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Percentage of responders - P10	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Percentage of responders - P11	Nonnormal	15.38 ± 10.41	13.79 ± 6.51	0 ± 0	1.308	0.281	0.268	−	−	−	0.001	0.971	0.050	3.054	0.057	0.561
Percentage of responders - P12	Nonnormal	53.84 ± 14.39	13.79 ± 6.51	0 ± 0	8.700	0.001	0.959	0.001	0.000	0.178	0.488	0.488	0.105	1.584	0.217	0.318
Percentage of responders - P13	Nonnormal	53.84 ± 14.39	55.17 ± 9.39	10 ± 10	3.045	0.058	0.560	0.969	0.043	0.023	0.238	0.628	0.077	1.082	0.348	0.228
Percentage of responders - P14	Nonnormal	100 ± 0	86.2 ± 6.51	60 ± 16.32	3.161	0.052	0.577	0.265	0.016	0.072	0.000	0.990	0.050	0.009	0.991	0.051
Percentage of responders - P15	Nonnormal	100 ± 0	100 ± 0	70 ± 15.27	8.228	0.001	0.949	0.970	0.001	0.000	1.019	0.318	0.167	0.865	0.428	0.189
Percentage of responders - P16	−	100 ± 0	100 ± 0	100 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Percentage of responders - average	Nonnormal	51.92 ± 1.67	47.41 ± 1.31	36.66 ± 2.83	7.944	0.001	0.995	0.286	0.000	0.002	0.466	0.498	0.056	0.144	0.866	0.104
First day of two consecutive successes	Nonnormal	14.07 ± 0.26	14.41 ± 0.16	15.6 ± 0.33	12.867	0.000	0.941	0.070	0.000	0.000	0.058	0.811	0.102	0.358	0.701	0.071

Cliff aversion
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		3.957	0.000	0.995	−	−	−
Day × genotype effect		0.796	0.702	0.580	−	−	−
Day × gender effect		0.613	0.782	0.299	−	−	−
Day × genotype × gender effect		1.266	0.209	0.835	−	−	−
Genotype effect		1.355	0.269	0.276	−	−	−
Gender effect		0.218	0.643	0.074	−	−	−
Genotype × gender		0.116	0.891	0.067	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Time to turn (seconds) - P2	Nonnormal	23.61 ± 2.77	24.44 ± 1.86	21.77 ± 3.56	0.745	0.481	0.168	−	−	−	0.482	0.491	0.104	0.866	0.428	0.189
Time to turn (seconds) - P3	Nonnormal	15.76 ± 3.37	14.17 ± 2.06	14.7 ± 4.34	0.099	0.906	0.064	−	−	−	0.056	0.814	0.056	1.513	0.232	0.304
Time to turn (seconds) - P4	Nonnormal	8.61 ± 3	4.93 ± 1.35	7.6 ± 3.73	0.963	0.390	0.206	−	−	−	0.340	0.563	0.088	1.916	0.160	0.376
Time to turn (seconds) - P5	Nonnormal	6.84 ± 2.86	7.03 ± 1.69	8.4 ± 3.61	0.156	0.856	0.073	−	−	−	0.898	0.349	0.153	2.242	0.119	0.432
Time to turn (seconds) - P6	Nonnormal	11.15 ± 3.42	8.75 ± 2.07	9.6 ± 3.55	0.126	0.882	0.068	−	−	−	0.040	0.843	0.054	2.223	0.121	0.429
Time to turn (seconds) - P7	Nonnormal	14.38 ± 3.83	9.75 ± 2.21	10 ± 4.36	1.057	0.356	0.223	−	−	−	1.259	0.268	0.195	0.368	0.694	0.105
Time to turn (seconds) - P8	Nonnormal	12.61 ± 3.97	4.82 ± 1.34	6.55 ± 3.08	2.580	0.087	0.488		0.144	0.788	0.618	0.436	0.120	0.315	0.731	0.097
Time to turn (seconds) - P9	Nonnormal	10.69 ± 3.19	9.72 ± 2.18	3.8 ± 0.92	1.129	0.333	0.236	−	−	−	0.011	0.917	0.051	0.285	0.753	0.092
Time to turn (seconds) - P10	Nonnormal	13.46 ± 3.45	7.03 ± 1.62	5.6 ± 2.77	2.447	0.098	0.466	0.048	0.076	0.770	1.109	0.298	0.177	0.316	0.731	0.097
Time to turn (seconds) - P11	Nonnormal	9.3 ± 2.66	11.51 ± 2.38	9 ± 3.55	0.634	0.535	0.149	−	−	−	0.263	0.611	0.079	1.486	0.238	0.300
Time to turn (seconds) - P12	Nonnormal	8.61 ± 1.52	8.93 ± 1.67	5.3 ± 1.21	0.467	0.630	0.121	−	−	−	0.001	0.975	0.050	0.651	0.527	0.152
Time to turn (seconds) - P13	Nonnormal	5.46 ± 1.7	6.48 ± 1.42	5.3 ± 2.78	0.125	0.883	0.068	−	−	−	2.134	0.151	0.298	0.791	0.460	0.176
Time to turn (seconds) - P14	Nonnormal	5.76 ± 2.16	4.1 ± 0.67	4.3 ± 1.67	0.605	0.551	0.144	−	−	−	1.345	0.253	0.205	0.941	0.398	0.202
Number of falls	Nonnormal	1.07 ± 0.53	0.44 ± 0.11	0.6 ± 0.26	1.568	0.220	0.314	−	−	−	3.688	0.061	0.467	1.125	0.334	0.235
First day of two consecutive successes (10-s cutoff)	Nonnormal	4.84 ± 0.29	4.75 ± 0.22	5.2 ± 0.55	0.546	0.583	0.134	−	−	−	2.726	0.106	0.365	2.174	0.126	0.421
First day of two consecutive successes (30-s cutoff)	Nonnormal	4.38 ± 0.33	4.06 ± 0.14	4.5 ± 0.45	1.370	0.265	0.279	−	−	−	0.037	0.849	0.054	1.044	0.361	0.221
Time to turn (seconds) - mean	Nonnormal	11.25 ± 1.25	9.36 ± 0.65	8.5 ± 0.85	1.315	0.279	0.269	−	−	−	0.259	0.613	0.079	0.119	0.888	0.067


Day effect		5.197	0.000	0.994	−	−	−
Day × genotype effect		0.866	0.581	0.502	−	−	−
Day × gender effect		0.830	0.547	0.325	−	−	−
Day × genotype × gender effect		1.115	0.348	0.637	−	−	−
Genotype effect		2.147	0.129	0.416	−	−	−
Gender effect		0.152	0.698	0.067	−	−	−
Genotype × gender		0.834	0.441	0.184	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Percentage of responders - P7	Nonnormal	46.15 ± 14.39	17.24 ± 7.13	50 ± 16.66	2.610	0.085	0.493	0.076	0.870	0.073	0.347	0.559	0.089	0.888	0.419	0.193
Percentage of responders - P8	Nonnormal	30.76 ± 13.32	6.89 ± 4.78	30 ± 15.27	2.340	0.108	0.449	−	−	−	0.089	0.766	0.060	0.834	0.441	0.184
Percentage of responders - P9	Nonnormal	46.15 ± 14.39	17.24 ± 7.13	10 ± 10	2.860	0.068	0.532	0.046	0.037	0.551	0.244	0.624	0.077	0.368	0.694	0.105
Percentage of responders - P10	Nonnormal	38.46 ± 14.04	24.13 ± 8.08	30 ± 15.27	0.394	0.677	0.110	−	−	−	3.054	0.088	0.401	1.829	0.173	0.361
Percentage of responders - P11	Nonnormal	53.84 ± 14.39	62.06 ± 9.16	70 ± 15.27	0.461	0.633	0.121	−	−	−	0.143	0.707	0.066	2.804	0.071	0.524
Percentage of responders - P12	Nonnormal	46.15 ± 14.39	41.37 ± 9.3	50 ± 16.66	0.214	0.808	0.081	−	−	−	0.527	0.472	0.109	0.270	0.765	0.090
Percentage of responders - P13	Nonnormal	46.15 ± 14.39	48.27 ± 9.44	60 ± 16.32	0.064	0.938	0.059	−	−	−	1.507	0.226	0.225	0.890	0.418	0.194
Percentage of responders - P14	Nonnormal	61.53 ± 14.04	72.41 ± 8.44	80 ± 13.33	0.379	0.687	0.107	−	−	−	0.022	0.882	0.052	0.468	0.629	0.122
Percentage of responders - P15	Nonnormal	100 ± 0	96.55 ± 3.44	90 ± 10	0.497	0.612	0.126	−	−	−	0.569	0.455	0.114	2.150	0.129	0.417
Percentage of responders - Average	Nonnormal	52.13 ± 5.39	42.91 ± 2.62	52.22 ± 3.33	2.147	0.129	0.416	−	−	−	0.152	0.698	0.067	0.834	0.441	0.184
First day of two consecutive successes	Nonnormal	9.23 ± 0.63	10.13 ± 0.36	8.8 ± 0.61	1.851	0.169	0.365	−	−	−	0.137	0.713	0.065	1.106	0.340	0.232

Rooting reflex
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		8.013	0.000	0.999	−	−	−
Day × genotype effect		1.657	0.107	0.735	−	−	−
Day × gender effect		0.847	0.503	0.276	−	−	−
Day × genotype × gender effect		1.347	0.219	0.625	−	−	−
Genotype effect		1.689	0.196	0.336	−	−	−
Gender effect		4.277	0.045	0.525	−	−	−
Genotype × gender		0.283	0.755	0.092	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Percentage of responders - P2	Nonnormal	23.07 ± 12.16	6.89 ± 4.78	20 ± 13.33	1.259	0.294	0.259	−	−	−	2.018	0.163	0.285	2.605	0.085	0.492
Percentage of responders - P3	Nonnormal	38.46 ± 14.04	34.48 ± 8.98	20 ± 13.33	0.643	0.531	0.151	−	−	−	1.878	0.177	0.268	0.771	0.469	0.173
Percentage of responders - P4	Nonnormal	46.15 ± 14.39	58.62 ± 9.3	50 ± 16.66	0.292	0.748	0.093	−	−	−	0.701	0.407	0.130	2.220	0.121	0.429
Percentage of responders - P5	Nonnormal	61.53 ± 14.04	82.75 ± 7.13	60 ± 16.32	1.489	0.237	0.301	−	−	−	3.072	0.087	0.403	0.308	0.736	0.096
Percentage of responders - P6	Nonnormal	92.3 ± 7.69	68.96 ± 8.74	70 ± 15.27	1.499	0.234	0.302	−	−	−	3.120	0.084	0.408	0.395	0.676	0.110
Percentage of responders - P7	Nonnormal	84.61 ± 10.41	68.96 ± 8.74	90 ± 10	1.161	0.323	0.242	−	−	−	0.959	0.333	0.160	1.595	0.214	0.320
Percentage of responders - P8	Nonnormal	84.61 ± 10.41	58.62 ± 9.3	40 ± 16.32	2.196	0.123	0.425	−	−	−	0.618	0.436	0.120	0.193	0.826	0.078
Percentage of responders - P9	Nonnormal	76.92 ± 12.16	31.03 ± 8.74	40 ± 16.32	4.400	0.018	0.730	0.005	0.055	0.687	2.183	0.147	0.304	0.616	0.545	0.146
Percentage of responders - P10	Nonnormal	38.46 ± 14.04	13.79 ± 6.51	20 ± 13.33	1.743	0.187	0.346	−	−	−	0.010	0.919	0.051	1.945	0.155	0.381
Percentage of responders - P11	Nonnormal	0 ± 0	0 ± 0	10 ± 10	2.310	0.111	0.444	−	−	−	2.619	0.113	0.353	2.258	0.117	0.435
Percentage of responders - P12	−	0 ± 0	0 ± 0	0 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Day of first observation	Nonnormal	4.15 ± 0.45	4.31 ± 0.28	5 ± 0.66	0.843	0.437	0.185	−	−	−	1.546	0.220	0.229	0.757	0.475	0.170
Day of last observation	Nonnormal	9.61 ± 0.56	8.58 ± 0.3	9.1 ± 0.48	1.599	0.214	0.320	−	−	−	1.428	0.239	0.215	1.200	0.311	0.249

Grasping reflex
Repeated measures, sphericity assumed		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		28.265	0.000	1.000	−	−	−
Day × genotype effect		1.038	0.415	0.591	−	−	−
Day × gender effect		0.534	0.850	0.208	−	−	−
Day × genotype × gender effect		1.356	0.150	0.725	−	−	−
Genotype effect		3.923	0.027	0.677	0.304	0.116	0.008
Gender effect		0.052	0.821	0.056	−	−	−
Genotype × gender		0.320	0.728	0.098	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Grasping score - P5	Nonnormal	1.3 ± 0.23	1.34 ± 0.16	1.6 ± 0.16	0.197	0.822	0.079	−	−	−	0.877	0.354	0.150	2.268	0.115	0.437
Grasping score - P6	Nonnormal	2.46 ± 0.36	2.93 ± 0.21	2.8 ± 0.38	0.580	0.564	0.140	−	−	−	0.184	0.670	0.070	0.595	0.556	0.143
Grasping score - P7	Nonnormal	2.92 ± 0.28	3.34 ± 0.17	2.9 ± 0.23	2.260	0.116	0.436	−	−	−	0.858	0.359	0.148	1.670	0.200	0.333
Grasping score - P8	Nonnormal	3.38 ± 0.28	3.41 ± 0.15	3.2 ± 0.24	0.316	0.731	0.097	−	−	−	1.283	0.264	0.198	1.183	0.316	0.246
Grasping score - P9	Nonnormal	3.76 ± 0.3	3.79 ± 0.09	3.8 ± 0.41	0.035	0.966	0.055	−	−	−	0.336	0.565	0.088	1.031	0.365	0.218
Grasping score - P10	Nonnormal	4.38 ± 0.24	4.65 ± 0.19	3.9 ± 0.37	2.102	0.134	0.409	−	−	−	0.035	0.852	0.054	1.782	0.180	0.353
Grasping score - P11	Nonnormal	5.3 ± 0.23	5.41 ± 0.15	4.9 ± 0.31	1.591	0.215	0.319	−	−	−	0.016	0.899	0.052	1.314	0.279	0.269
Grasping score - P12	Nonnormal	5.3 ± 0.23	5.37 ± 0.15	5 ± 0.29	0.477	0.624	0.123	−	−	−	0.028	0.868	0.053	0.393	0.678	0.109
Grasping score - P13	Nonnormal	5.69 ± 0.17	5.86 ± 0.09	5.2 ± 0.24	3.789	0.030	0.660	0.399	0.092	0.009	1.328	0.255	0.204	0.287	0.752	0.093
Grasping score - P14	Nonnormal	5.69 ± 0.13	5.68 ± 0.12	4.6 ± 0.26	10.311	0.000	0.982	0.945	0.000	0.000	0.034	0.855	0.054	0.334	0.718	0.100
Grasping score - Average	Nonnormal	4.02 ± 0.13	4.18 ± 0.07	3.79 ± 0.13	3.923	0.027	0.677	0.304	0.116	0.008	0.052	0.821	0.056	0.320	0.728	0.098
First day of two consecutive successes (score 4)	Nonnormal	9.38 ± 0.43	8.75 ± 0.29	10.9 ± 0.37	10.068	0.000	0.979	0.233	0.005	0.000	0.131	0.719	0.065	1.318	0.278	0.270

Day effect		21.337	0.000	1.000	−	−	−
Day × genotype effect		0.988	0.460	0.563	−	−	−
Day × gender effect		0.921	0.478	0.356	−	−	−
Day × genotype × gender effect		0.688	0.758	0.390	−	−	−
Genotype effect		1.593	0.215	0.319	−	−	−
Gender effect		0.857	0.360	0.148	−	−	−
Genotype × gender		0.865	0.428	0.189	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Time to turn (seconds) - P2	Nonnormal	18.15 ± 2.97	19.93 ± 2.09	24.2 ± 2.64	0.933	0.401	0.201	−	−	−	0.382	0.540	0.093	1.161	0.323	0.242
Time to turn (seconds) - P3	Nonnormal	20.76 ± 3.19	18.13 ± 1.98	19.7 ± 3.05	0.398	0.674	0.110	−	−	−	0.020	0.887	0.052	1.146	0.327	0.239
Time to turn (seconds) - P4	Nonnormal	23.61 ± 2.26	18.03 ± 2.12	22.8 ± 3.71	1.506	0.233	0.304	−	−	−	0.078	0.781	0.059	1.392	0.259	0.283
Time to turn (seconds) - P5	Nonnormal	18.61 ± 3.27	16.82 ± 2.19	25.5 ± 2.29	2.431	0.100	0.464	−	−	−	0.284	0.597	0.082	0.129	0.879	0.069
Time to turn (seconds) - P6	Nonnormal	18.69 ± 3.26	14.2 ± 2.28	18.8 ± 3.14	1.242	0.299	0.256	−	−	−	0.163	0.688	0.068	0.079	0.925	0.061
Time to turn (seconds) - P7	Nonnormal	10.38 ± 3.18	8.37 ± 1.72	9 ± 3.16	0.155	0.857	0.072	−	−	−	4.992	0.031	0.589	0.741	0.482	0.168
Time to turn (seconds) - P8	Nonnormal	5 ± 1.91	5.34 ± 1.27	4.5 ± 1.43	0.073	0.930	0.060	−	−	−	3.064	0.087	0.402	0.023	0.978	0.053
Time to turn (seconds) - P9	Nonnormal	3 ± 0.62	4.24 ± 1.09	3.3 ± 0.83	0.397	0.675	0.110	−	−	−	0.371	0.546	0.092	0.413	0.664	0.113
Time to turn (seconds) - P10	Nonnormal	1.3 ± 0.13	2.06 ± 0.43	1.5 ± 0.16	0.901	0.414	0.196	−	−	−	0.002	0.966	0.050	0.059	0.943	0.058
Time to turn (seconds) - P11	Nonnormal	1 ± 0	1.27 ± 0.15	1.3 ± 0.15	1.320	0.277	0.270	−	−	−	0.034	0.854	0.054	0.314	0.732	0.097
Time to turn (seconds) - P12	Nonnormal	1 ± 0	1.03 ± 0.03	1 ± 0	0.398	0.674	0.110	−	−	−	0.331	0.568	0.087	0.196	0.823	0.079
Time to turn (seconds) - P13	−	1 ± 0	1 ± 0	1 ± 0	−	−	−	−	−	−	−	−	−	−	−	−
Time to turn (seconds) - Mean	Normal	10.21 ± 1.03	9.2 ± 0.59	11.05 ± 0.79	1.593	0.215	0.319	−	−	−	0.857	0.360	0.148	0.865	0.428	0.189
Time to turn (days) - first day of two consecutive successes	Nonnormal	9.61 ± 0.26	9.44 ± 0.34	9.7 ± 0.36	0.139	0.871	0.070	−	−	−	3.774	0.058	0.476	1.174	0.319	0.244

Negative geotaxis
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		12.128	0.000	1.000	−	−	−
Day × genotype effect		1.526	0.086	0.895	−	−	−
Day × gender effect		1.036	0.409	0.488	−	−	−
Day × genotype × gender effect		1.386	0.144	0.855	−	−	−
Genotype effect		2.110	0.133	0.410	−	−	−
Gender effect		0.493	0.486	0.106	−	−	−
Genotype × gender		0.090	0.914	0.063	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Time to turn (seconds) - P2	Nonnormal	-9.66 ± 5.21	-18.82 ± 2.48	-11.6 ± 4.73	1.821	0.174	0.360	−	−	−	3.882	0.055	0.487	1.077	0.350	0.227
Time to turn (seconds) - P3	Nonnormal	-8.69 ± 5.99	-15.58 ± 2.88	-11.8 ± 5.61	0.847	0.436	0.186	−	−	−	0.443	0.509	0.100	0.364	0.697	0.105
Time to turn (seconds) - P4	Nonnormal	-6 ± 5.23	-10.82 ± 1.86	-15.2 ± 3.51	1.537	0.226	0.309	−	−	−	0.656	0.422	0.124	4.540	0.016	0.744
Time to turn (seconds) - P5	Nonnormal	9.69 ± 3.67	-4.2 ± 3.15	-2.2 ± 5.3	4.418	0.018	0.732	0.006	0.033	0.936	0.399	0.531	0.095	0.766	0.471	0.172
Time to turn (seconds) - P6	Nonnormal	6.84 ± 4.38	3.2 ± 2.97	8.5 ± 3.87	0.517	0.600	0.130	−	−	−	0.010	0.920	0.051	0.905	0.412	0.196
Time to turn (seconds) - P7	Nonnormal	6.3 ± 4.2	6.31 ± 3.13	12.4 ± 5.82	0.281	0.756	0.092	−	−	−	0.699	0.408	0.129	0.013	0.987	0.052
Time to turn (seconds) - P8	Nonnormal	8.3 ± 4.42	11.44 ± 2.71	6.8 ± 6.1	0.331	0.720	0.100	−	−	−	0.124	0.726	0.064	0.862	0.429	0.189
Time to turn (seconds) - P9	Nonnormal	12.76 ± 3.75	12.17 ± 2.44	23.1 ± 1.6	3.787	0.030	0.660	0.828	0.035	0.010	0.023	0.880	0.053	1.215	0.307	0.251
Time to turn (seconds) - P10	Nonnormal	9.84 ± 2.94	10.03 ± 2.29	19 ± 3.22	2.707	0.078	0.508	0.959	0.055	0.032	2.235	0.142	0.310	0.497	0.612	0.126
Time to turn (seconds) - P11	Nonnormal	10.92 ± 3.44	13.62 ± 2.17	15 ± 3.88	0.580	0.564	0.140	−	−	−	2.444	0.125	0.334	4.258	0.020	0.715
Time to turn (seconds) - P12	Nonnormal	11.92 ± 2.92	15.96 ± 1.74	22.2 ± 1.33	3.269	0.047	0.592	0.379	0.014	0.073	0.205	0.653	0.073	2.337	0.109	0.448
Time to turn (seconds) - P13	Nonnormal	15.23 ± 3.04	18.58 ± 1.5	24.1 ± 0.62	3.112	0.054	0.570	0.219	0.017	0.091	1.431	0.238	0.216	1.112	0.338	0.233
Time to turn (seconds) - P14	Nonnormal	22.69 ± 1.43	21.82 ± 1.17	22.3 ± 2.25	0.135	0.874	0.070	−	−	−	2.267	0.139	0.313	1.471	0.241	0.297
Time to turn (seconds) - Mean	Normal	6.99 ± 1.25	4.9 ± 1.06	8.66 ± 1.1	2.110	0.133	0.410	−	−	−	0.493	0.486	0.106	0.090	0.914	0.063
Falls	Nonnormal	9 ± 0.83	10.31 ± 0.48	9.1 ± 0.45	1.527	0.228	0.307	−	−	−	0.192	0.664	0.071	0.307	0.737	0.096

Air righting
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		21.651	0.000	1.000	−	−	−
Day × genotype effect		3.211	0.001	0.986	−	−	−
Day × gender effect		2.423	0.037	0.760	−	−	−
Day × genotype × gender effect		1.309	0.227	0.664	−	−	−
Genotype effect		3.166	0.052	0.577	0.693	0.070	0.016
Gender effect		0.464	0.499	0.102	−	−	−
Genotype × gender		1.482	0.238	0.299	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Air righting score - P8	Nonnormal	0.61 ± 0.26	0.41 ± 0.13	0.8 ± 0.29	1.160	0.323	0.242	−	−	−	5.791	0.020	0.653	5.562	0.007	0.831
Air righting score - P9	Nonnormal	0.38 ± 0.21	0.93 ± 0.17	0.4 ± 0.22	2.272	0.115	0.438	−	−	−	0.004	0.948	0.050	0.439	0.648	0.117
Air righting score - P10	Nonnormal	1.69 ± 0.2	1.44 ± 0.13	0.7 ± 0.3	5.755	0.006	0.844	0.426	0.003	0.005	1.456	0.234	0.219	0.438	0.648	0.117
Air righting score - P11	Nonnormal	1.3 ± 0.26	1.44 ± 0.16	0.4 ± 0.26	5.407	0.008	0.819	0.703	0.015	0.002	2.641	0.111	0.356	0.639	0.533	0.150
Air righting score - P12	Nonnormal	1.84 ± 0.15	1.89 ± 0.05	1.4 ± 0.22	4.066	0.024	0.693	0.742	0.034	0.007	0.332	0.567	0.087	0.647	0.528	0.152
Air righting score - P13	Nonnormal	1.76 ± 0.16	1.68 ± 0.13	1.7 ± 0.21	0.069	0.934	0.060	−	−	−	2.104	0.154	0.295	0.396	0.675	0.110

Air righting score - P14	Nonnormal	2 ± 0	2 ± 0	2 ± 0	−	−	.	−	−	−	−	−	.	−	−	.
Air righting score - P15	Nonnormal	1.92 ± 0.07	2 ± 0	2 ± 0	1.621	0.209	0.324	−	−	−	1.716	0.197	0.249	1.464	0.242	0.296
Air righting score - P16	Nonnormal	2 ± 0	2 ± 0	2 ± 0	−	−	.	−	−	−	−	−	.			.
Air righting score - P17	Nonnormal	2 ± 0	1.96 ± 0.03	2 ± 0	0.398	0.674	0.110	−	−	−	0.331	0.568	0.087	0.196	0.823	0.079
Air righting score - P18	Nonnormal	2 ± 0	2 ± 0	2 ± 0	−	−	.	−	−	−	−	−	.	−	−	.
Air righting score - P19	Nonnormal	2 ± 0	2 ± 0	2 ± 0	−	−	.	−	−	−	−	−	.	−	−	.
Air righting score - P20	Nonnormal	2 ± 0	2 ± 0	2 ± 0	−	−	.	−	−	−	−	−	.	−	−	.
Air righting score - Mean	Nonnormal	1.65 ± 0.06	1.67 ± 0.03	1.49 ± 0.06	3.166	0.049	0.577	0.693	0.070	0.016	0.464	0.499	0.102	1.482	0.238	0.299
First day of two consecutive successes	Nonnormal	11.84 ± 0.5	11.37 ± 0.29	12.9 ± 0.56	2.814	0.071	0.525	0.378	0.184	0.023	0.959	0.333	0.160	1.173	0.319	0.244

Wire suspension
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		16.511	0.000	1.000	−	−	−
Day × genotype effect		3.538	0.000	0.994	−	−	−
Day × gender effect		0.497	0.782	0.186	−	−	−
Day × genotype × gender effect		0.635	0.787	0.333	−	−	−
Genotype effect		13.553	0.000	0.997	0.013	0.000	0.001
Gender effect		0.303	0.585	0.084	−	−	−
Genotype × gender		2.871	0.067	0.534	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Suspension time (seconds) - P11	Nonnormal	5.15 ± 1.44	4.37 ± 0.63	2.7 ± 0.47	1.701	0.194	0.338	−	−	−	0.045	0.833	0.055	0.287	0.752	0.093
Suspension time (seconds) - P12	Nonnormal	3.23 ± 1.06	3.13 ± 0.45	3.9 ± 1.65	0.220	0.803	0.082	−	−	−	0.856	0.360	0.148	0.662	0.521	0.154
Suspension time (seconds) - P13	Nonnormal	2.69 ± 0.47	4 ± 0.52	2.8 ± 0.87	1.660	0.202	0.331	−	−	−	2.518	0.120	0.342	0.036	0.965	0.055
Suspension time (seconds) - P14	Nonnormal	7.61 ± 1.97	5.17 ± 0.58	3.7 ± 1.12	2.196	0.123	0.425	−	−	−	0.006	0.938	0.051	1.893	0.163	0.372
Suspension time (seconds) - P15	Nonnormal	9.92 ± 2.29	4.82 ± 0.53	1.7 ± 0.42	10.137	0.000	0.980	0.002	0.000	0.054	0.290	0.593	0.082	0.611	0.547	0.146
Suspension time (seconds) - P16	Nonnormal	13.38 ± 2.18	6.41 ± 0.53	3.7 ± 0.91	15.666	0.000	0.999	0.000	0.000	0.100	0.744	0.393	0.135	1.971	0.151	0.386
Suspension time (seconds) - P17	Nonnormal	18.53 ± 2.34	11.82 ± 1.22	9.3 ± 1.6	6.683	0.003	0.896	0.004	0.002	0.288	0.538	0.467	0.111	1.214	0.307	0.251
Suspension time (seconds) - P18	Nonnormal	16.15 ± 2.22	18.34 ± 1.78	11.1 ± 2.37	2.398	0.103	0.459	−	−	−	0.551	0.462	0.112	0.722	0.491	0.164
Suspension time (seconds) - P19	Nonnormal	18.38 ± 2.11	19.48 ± 1.73	8 ± 1.97	7.474	0.002	0.927	0.921	0.003	0.001	0.000	0.995	0.050	1.609	0.212	0.322
Suspension time (seconds) - P20	Nonnormal	17.07 ± 2.31	12.13 ± 1.54	5.6 ± 0.85	6.858	0.003	0.903	0.053	0.001	0.019	0.646	0.426	0.123	1.283	0.287	0.264
Suspension time (seconds) - Average	Nonnormal	11.21 ± 1	8.97 ± 0.55	5.25 ± 0.54	13.553	0.000	0.997	0.013	0.000	0.001	0.303	0.585	0.084	2.871	0.067	0.534
Suspension time (seconds) - Best score	Nonnormal	24.3 ± 2.06	23.31 ± 1.37	13.7 ± 1.99	7.828	0.001	0.938	0.525	0.001	0.001	0.168	0.684	0.069	1.205	0.309	0.250

Open field
Repeated measures, sphericity violated		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		31.056	0.000	1.000	−	−	−
Day × genotype effect		0.874	0.572	0.501	−	−	−
Day × gender effect		0.630	0.702	0.247	−	−	−
Day × genotype × gender effect		1.857	0.042	0.887	−	−	−
Genotype effect		0.117	0.890	0.067	−	−	−
Gender effect		0.046	0.831	0.055	−	−	−
Genotype × gender		1.755	0.185	0.348	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Time to escape (seconds) - P8	Nonnormal	26.07 ± 2.09	27.55 ± 1.19	28.9 ± 0.99	0.473	0.626	0.122	−	−	−	0.928	0.341	0.156	0.010	0.990	0.051
Time to escape (seconds) - P9	Nonnormal	23.3 ± 2.82	21.34 ± 1.83	24.2 ± 2.64	0.305	0.739	0.095	−	−	−	0.774	0.384	0.138	0.929	0.403	0.201
Time to escape (seconds) - P10	Nonnormal	20.46 ± 2.55	19.96 ± 1.72	22.1 ± 2.34	0.286	0.753	0.093	−	−	−	0.010	0.922	0.051	1.615	0.210	0.323
Time to escape (seconds) - P11	Nonnormal	22.07 ± 2.18	17.55 ± 1.94	20.2 ± 3.04	0.938	0.399	0.202	−	−	−	1.030	0.316	0.168	3.307	0.046	0.597
Time to escape (seconds) - P12	Nonnormal	17 ± 2.34	19.27 ± 1.49	16.2 ± 2.09	0.660	0.522	0.154	−	−	−	0.718	0.401	0.132	1.010	0.372	0.215
Time to escape (seconds) - P13	Nonnormal	15.61 ± 2.38	18.2 ± 1.72	15.6 ± 2.25	0.573	0.568	0.139	−	−	−	0.015	0.902	0.052	0.685	0.509	0.158
Time to escape (seconds) - P14	Nonnormal	17.23 ± 2.78	12.72 ± 1.6	10.6 ± 1.14	2.349	0.107	0.450	−	−	−	0.421	0.520	0.097	5.349	0.008	0.815
Time to escape (seconds) - P15	Nonnormal	4.92 ± 0.38	5.65 ± 0.39	7 ± 1.46	1.768	0.183	0.350	−	−	−	0.215	0.645	0.074	0.019	0.981	0.053
Time to escape (seconds) - P16	Nonnormal	3.61 ± 0.28	3.65 ± 0.25	4.5 ± 0.87	1.119	0.336	0.234	−	−	−	2.068	0.158	0.290	0.043	0.958	0.056
Time to escape (seconds) - P17	Nonnormal	2.46 ± 0.24	3.48 ± 0.26	3.6 ± 0.37	3.840	0.029	0.667	0.014	0.026	0.745	1.782	0.189	0.257	1.179	0.317	0.245
Time to escape (seconds) - P18	Nonnormal	2.23 ± 0.32	2.1 ± 0.21	2.1 ± 0.4	0.028	0.972	0.054	−	−	−	0.121	0.730	0.063	0.313	0.733	0.097
Time to escape (seconds) - P19	Nonnormal	2.38 ± 0.33	2.17 ± 0.29	2 ± 0.36	0.011	0.989	0.051	−	−	−	1.947	0.170	0.276	2.592	0.086	0.490
Time to escape (seconds) - P20	Nonnormal	1.92 ± 0.21	2.13 ± 0.16	1.9 ± 0.17	0.632	0.536	0.149	−	−	−	0.044	0.834	0.055	0.205	0.816	0.080
Time to escape (seconds) - average	Normal	12.25 ± 0.59	11.98 ± 0.49	12.22 ± 0.53	0.117	0.890	0.067	−	−	−	0.046	0.831	0.055	1.755	0.185	0.348
First day of two consecutive successes (30-s cutoff)	Nonnormal	11.92 ± 0.58	11.24 ± 0.32	11.5 ± 0.4	0.589	0.559	0.142	−	−	−	0.701	0.407	0.130	0.297	0.745	0.094

Ultrasonic vocalizations
Number of calls - repeated measures, sphericity assumed		F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Day effect		4.600	0.012	0.767	−	−	−
Day × genotype effect		0.991	0.416	0.303	−	−	−
Day × gender effect		1.430	0.244	0.300	−	−	−
Day × genotype × gender effect		0.305	0.874	0.116	−	−	−

Genotype effect		0.533	0.590	0.133	−	−	−
Gender effect		1.697	0.199	0.248	−	−	−
Genotype × gender		0.869	0.426	0.191	−	−	−
											Gender effect			Gender × genotype effect
Multifactiorial ANCOVA		WT	Het	KO	F	p value	Power	WT vs Het	WT vs KO	Het vs KO	F	p value	Power	F	p value	Power
Number of calls - minute 1	Nonnormal	13.81 ± 4.24	11.46 ± 2.89	15.88 ± 5.31	0.586	0.562	0.139	−	−	−	0.163	0.689	0.068	0.090	0.914	0.063
Number of calls - minute 2	Nonnormal	18.68 ± 5.15	11.56 ± 2.73	13.22 ± 4.78	0.567	0.572	0.136	−	−	−	0.327	0.571	0.086	0.068	0.935	0.059
Number of calls - minute 3	Nonnormal	15.75 ± 4.72	13.96 ± 3.72	8.33 ± 4.2	0.172	0.843	0.074	−	−	−	3.481	0.071	0.442	0.097	0.908	0.064
Number of calls - total	Nonnormal	48.43 ± 13.28	37.06 ± 7.85	37.44 ± 12.08	0.156	0.856	0.072	−	−	−	1.323	0.258	0.201	0.052	0.949	0.057
Calling time - minute 1	Nonnormal	0.93 ± 0.29	0.75 ± 0.19	1.08 ± 0.38	0.738	0.485	0.165	−	−	−	0.106	0.746	0.062	0.083	0.920	0.062
Calling time - minute 2	Nonnormal	1.24 ± 0.39	0.76 ± 0.19	0.85 ± 0.3	0.368	0.695	0.104	−	−	−	0.231	0.634	0.075	0.059	0.943	0.058
Calling time - minute 3	Nonnormal	1.16 ± 0.4	0.96 ± 0.25	0.53 ± 0.27	0.162	0.851	0.073	−	−	−	4.123	0.050	0.505	0.006	0.994	0.051
Calling time - total	Nonnormal	3.41 ± 1.05	2.48 ± 0.52	2.47 ± 0.81	0.189	0.828	0.077	−	−	−	1.431	0.240	0.213	0.023	0.977	0.053
Average duration - min 1	Nonnormal	0.05 ± 0	0.05 ± 0	0.03 ± 0.01	0.138	0.872	0.069	−	−	−	0.056	0.815	0.056	0.950	0.400	0.197
Average duration - min 2	Nonnormal	0.06 ± 0	0.06 ± 0	0.03 ± 0.01	0.319	0.730	0.095	−	−	−	1.879	0.182	0.262	1.561	0.229	0.301
Average duration - min 3	Nonnormal	0.06 ± 0	0.06 ± 0	0.02 ± 0.01	6.759	0.004	0.883	−	−	−	23.838	0.000	0.997	7.350	0.003	0.909
Average duration - total	Nonnormal	0.08 ± 0.01	0.06 ± 0	0.03 ± 0.01	0.515	0.604	0.125	−	−	−	1.663	0.209	0.237	0.847	0.440	0.179
Latency to first call	Nonnormal	77.28 ± 17.98	80.04 ± 12.54	75.57 ± 27.65	0.411	0.665	0.113	−	−	−	0.155	0.696	0.067	3.311	0.045	0.602
Mean frequency - total	Nonnormal	71337.77 ± 1902.04	73128.73 ± 3879.33	75883.81 ± 3957.75	0.024	0.976	0.053	−	−	−	0.036	0.851	0.054	0.029	0.971	0.054
Mean amplitude - total	Nonnormal	78.43 ± 29.11	66.33 ± 20.80	37.15 ± 17.64	0.189	0.829	0.076	−	−	−	2.440	0.130	0.325	0.442	0.648	0.114
Percentage of noncaller	Nonnormal	18.75 ± 10.08	28.13 ± 8.08	33.33 ± 16.67	0.401	0.672	0.111	−	−	−	0.587	0.447	0.117	1.933	0.155	0.382

WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Bold font indicates significant results (p < 0.05). P: postnatal day.