Detailed results and statistical analyses related to general health, physical factors, gross appearance, and spontaneous activity
| Physical factors and gross appearance | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| 3-months weight (g) | 2wANOVA | Normal | 26.33 ± 0.87 | 27.18 ± 0.57 | 26.01 ± 0.54 | 1.241 | 0.298 | 0.258 | 2.546 | 0.117 | 0.347 | 2.000 | 0.146 | 0.394 | − | − | − |
| 15-months weight (g) | 2wANOVA | Normal | 33.41 ± 2 | 31.06 ± 1.21 | 29.36 ± 1.73 | 1.578 | 0.222 | 0.310 | 0.269 | 0.608 | 0.079 | 0.491 | 0.617 | 0.123 | − | − | − |
| 20-months weight (g) | 2wANOVA | Normal | 32.9 ± 1.86 | 31.43 ± 1.2 | 28.84 ± 1.5 | 0.982 | 0.390 | 0.199 | 0.034 | 0.856 | 0.054 | 0.018 | 0.982 | 0.052 | − | − | − |
| Length | 2wANOVA | Nonnormal | 16.45 ± 0.24 | 16.84 ± 0.24 | 16.73 ± 0.25 | 1.062 | 0.353 | 0.226 | 91.207 | 0.000 | 1.000 | 0.471 | 0.627 | 0.123 | − | − | − |
| Coat appearance | 2wANOVA | Nonnormal | 2.63 ± 0.13 | 2.89 ± 0.07 | 2.94 ± 0.05 | 2.558 | 0.087 | 0.489 | 2.615 | 0.112 | 0.355 | 1.424 | 0.250 | 0.291 | 0.119 | 0.050 | 0.915 |
| Skin color | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Whisker barbering | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Missing fur on face | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Missing fur on body | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Wounding | 2wANOVA | Nonnormal | 0.15 ± 0.11 | 0 ± 0 | 0.05 ± 0.05 | 1.078 | 0.348 | 0.229 | 0.028 | 0.869 | 0.053 | 0.279 | 0.758 | 0.092 | − | − | − |
| Body tone | 2wANOVA | Nonnormal | 1.1 ± 0.07 | 1.1 ± 0.07 | 1 ± 0.07 | 0.563 | 0.573 | 0.138 | 2.225 | 0.142 | 0.310 | 0.563 | 0.573 | 0.138 | − | − | − |
| Palpebral closure | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Piloerection | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Jar observation | |||||||||||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vsKO | |
| Body position | 2wANOVA | Nonnormal | 4.15 ± 0.08 | 4.1 ± 0.07 | 4.26 ± 0.1 | 0.702 | 0.500 | 0.162 | 4.949 | 0.031 | 0.588 | 0.139 | 0.871 | 0.070 | − | − | − |
| Spontaneous activity | 2wANOVA | Nonnormal | 1.68 ± 0.1 | 1.57 ± 0.11 | 1.63 ± 0.11 | 0.223 | 0.801 | 0.083 | 0.282 | 0.598 | 0.082 | 0.279 | 0.758 | 0.092 | − | − | − |
| Latency to sit/stand (s) | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | − | − | − |
| Latency to rear (s) | 2wANOVA | Nonnormal | 8.94 ± 1.36 | 8.05 ± 1.03 | 5.63 ± 1.02 | 2.137 | 0.128 | 0.418 | 0.036 | 0.850 | 0.054 | 0.046 | 0.955 | 0.057 | − | − | − |
| Repeated jumps (%) | 2wANOVA | Nonnormal | 15.78 ± 8.59 | 10.52 ± 7.23 | 26.31 ± 10.37 | 0.702 | 0.500 | 0.162 | 4.949 | 0.031 | 0.588 | 0.139 | 0.871 | 0.070 | − | − | − |
| Circling (%) | 2wANOVA | Nonnormal | 5.26 ± 5.26 | 10.52 ± 7.23 | 10.52 ± 7.23 | 0.289 | 0.750 | 0.093 | 5.177 | 0.027 | 0.607 | 0.289 | 0.750 | 0.093 | − | − | − |
| Urination | 2wANOVA | Nonnormal | 0.47 ± 0.19 | 0.1 ± 0.1 | 0.15 ± 0.11 | 1.540 | 0.224 | 0.312 | 0.023 | 0.881 | 0.052 | 1.213 | 0.306 | 0.253 | − | − | − |
| Defecation (number) | 2wANOVA | Nonnormal | 1.57 ± 0.35 | 0.94 ± 0.29 | 0.94 ± 0.27 | 1.065 | 0.352 | 0.226 | 0.003 | 0.958 | 0.050 | 2.592 | 0.085 | 0.494 | − | − | − |
| Respiration | − | − | 2 ± 0 | 2 ± 0 | 2 ± 0 | − | − | − | − | − | − | − | − | − | |||
| Tremor | − | − | 0 ± 0 | 0 ± 0 | 0 ± 0 | − | − | − | − | − | − | − | − | − | |||
| Cage transfer | |||||||||||||||||
| Genotype | Cohort | Genotype × cohort | Pairwise comparisons | ||||||||||||||
| Test | Data structure | WT | Het | KO | F | p value | Power | F | p value | Power | F | p value | Power | WT vs Het | WT vs KO | Het vs KO | |
| Transfer arousal | 2wANOVA | Nonnormal | 3.21 ± 0.22 | 3.21 ± 0.22 | 3.15 ± 0.2 | 0.037 | 0.964 | 0.055 | 1.470 | 0.231 | 0.221 | 2.055 | 0.139 | 0.404 | − | − | − |
| Gait | 2wANOVA | Nonnormal | 0 ± 0 | 0.15 ± 0.08 | 0.05 ± 0.05 | 1.783 | 0.178 | 0.356 | 0.003 | 0.957 | 0.050 | 0.637 | 0.533 | 0.151 | − | − | − |
| Pelvic elevation | 2wANOVA | Nonnormal | 2 ± 0 | 2.15 ± 0.08 | 2 ± 0.07 | 1.730 | 0.188 | 0.347 | 0.141 | 0.709 | 0.066 | 0.141 | 0.869 | 0.071 | − | − | − |
| Tail elevation | 2wANOVA | Nonnormal | 1.89 ± 0.15 | 1.73 ± 0.18 | 1.21 ± 0.18 | 4.003 | 0.024 | 0.691 | 4.469 | 0.039 | 0.545 | 0.204 | 0.816 | 0.080 | 0.530 | 0.009 | 0.043 |
WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Bold font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 5-1. 2wANOVA: 2-way ANOVA.