Table 11.

Detailed results and statistical analyses related to stereotypies, repetitive behavior, perseveration, and cognitive flexibility

Sterotypies in open field
GenotypeCohortGenotype × cohortPairwise comparisons
TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Grooming, time (s)2wANOVANonnormal67.01 ± 7.0162.6 ± 5.5892.49 ± 10.454.9290.0110.78422.8060.0000.9972.5300.0900.4840.8830.0230.006
Grooming, number2wANOVANormal25.42 ± 1.5122.26 ± 1.527.1 ± 1.932.0000.1460.3941.4020.2420.2131.7450.1850.349
Jumping, time (s)2wANOVANonnormal0.07 ± 0.050 ± 00.18 ± 0.11.6660.1990.3350.0330.8570.0541.1490.3250.242
Jumping, number2wANOVANonnormal0.36 ± 0.230 ± 00.42 ± 0.231.3000.2810.2690.1550.6960.0671.8160.1730.362
Rotation, time (s)2wANOVANonnormal0.39 ± 0.11.49 ± 0.814.21 ± 2.761.5600.2200.3162.0690.1560.2921.0380.3610.222
Rotation, number2wANOVANonnormal1.63 ± 0.392.21 ± 0.466.15 ± 1.825.8830.0050.8563.3010.0750.4303.0220.0570.5610.9200.0100.028
Twitching/shaking, time (s)2wANOVANonnormal0.28 ± 0.070.69 ± 0.330.63 ± 0.11.0890.3440.2310.5400.4660.1110.8790.4220.193
Twitching/shaking, number2wANOVANonnormal1.73 ± 0.422.63 ± 0.883 ± 0.471.1940.3110.2501.4840.2290.2230.5890.5590.143
Repetitive novel object contact task, object preference, time
Object exploration, time (s)TestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- Object rMeasuresSph.viol10.5330.0000.999
- Object × gen. rMeasuresSph.viol2.1500.0690.753
- GenotyperMeasuresSph.viol7.9640.0010.9430.9560.0140.006
- CohortrMeasuresSph.viol24.6540.0000.998
- Object × gen. × coh.rMeasuresSph.viol0.3660.8590.152
- Gen. × coh.rMeasuresSph.viol0.6470.5280.152
GenotypeCohortGenotype × cohortPairwise comparisons
Object explorationTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Dice, time (s)2wANOVANonnormal16.51 ± 2.4914.82 ± 1.6612.14 ± 1.791.7480.1850.3494.6400.0360.5600.8330.4410.185
Jack, time (s)2wANOVANonnormal21.78 ± 3.1827.99 ± 5.115.39 ± 24.0780.0230.69714.1580.0000.9580.5000.6100.1270.3110.4430.025
Lego, time (s)2wANOVANonnormal24.91 ± 3.5928.25 ± 2.9414.97 ± 1.878.6220.0010.95920.9650.0000.9940.7740.4670.1740.5090.0310.001
Pin, time (s)2wANOVANonnormal20.8 ± 4.313.72 ± 1.8210.86 ± 1.73.1990.0500.5854.5320.0380.5500.0570.9440.0580.2440.0670.755
Object exploration, %TestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- Object rMeasuresSph.ass8.3290.0000.985
- Object × gen.rMeasuresSph.ass0.7210.6330.259
- GenotyperMeasuresSph.ass0.7500.4780.170
- CohortrMeasuresSph.ass0.0001.0000.050
- Object × gen. × coh.rMeasuresSph.ass0.6520.6880.236
- Gen. × coh.rMeasuresSph.ass0.0001.0000.050
GenotypeCohortGenotype × cohortPairwise comparisons
Object explorationTestData structureWTHetKOFp valuePowerFpvaluePowerFp valuePowerWT vs HetWT vs KOHet vsKO
Dice, time (%)2wANOVANonnormal20.09 ± 1.7819.13 ± 2.1124.11 ± 3.721.2590.2930.2614.6350.0360.5601.1790.3160.246
Jack, time (%)2wANOVANonnormal26.86 ± 3.330.29 ± 3.6128.38 ± 3.260.2210.8030.0831.5290.2220.2280.0220.9780.053
Lego, time (%)2wANOVANormal29.85 ± 2.1932.98 ± 2.2827.98 ± 2.51.1740.3180.2450.9860.3260.1640.0720.9310.060
Pin, time (%)2wANOVANonnormal23.18 ± 3.0217.57 ± 2.6519.51 ± 2.320.7010.5010.1610.1860.6690.0711.6840.1960.337
Object exploration ranked by preferenceTestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- ObjectrMeasuresSph.viol110.8870.0001.000
- Object × gen.rMeasuresSph.viol5.4830.0020.996
- GenotyperMeasuresSph.viol8.0540.0010.9460.9480.0140.006
- CohortrMeasuresSph.viol24.5780.0000.998
- Object × gen. × coh.rMeasuresSph.viol1.1870.3210.457
- Gen. × coh.rMeasuresSph.viol0.6430.5300.152
GenotypeCohortGenotype × cohortPairwise comparisons
Object explorationTestData structureWTHetKOFp valuePowerFp valuePowerFpvaluePowerWT vs HetWT vs KOHet vs KO
Object #1, time (s)2wANOVANonnormal31.83 ± 3.5937.45 ± 4.0920.53 ± 1.649.0510.0000.96715.0930.0000.9680.9340.4000.2020.2640.0480.001
Object #2, time (s)2wANOVANonnormal24.5 ± 3.2722.95 ± 2.1415.23 ± 1.786.7090.0030.89922.5290.0000.9960.4560.6370.1200.9410.0160.033
Object #3, time (s)2wANOVANonnormal15.68 ± 2.6915.29 ± 1.5610.48 ± 1.154.2240.0200.71420.0370.0000.9921.3060.2800.2690.9980.0940.102
Object #4, time (s)2wANOVANonnormal12 ± 2.419.34 ± 0.967.12 ± 0.993.7630.0300.66012.1550.0010.9270.6300.5370.1490.4510.0740.533
Object exploration,ranked by preferenceTestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- ObjectrMeasuresSph.viol146.5340.0001.000
- Object × gen.rMeasuresSph.viol0.8320.4900.321
- GenotyperMeasuresSph.viol0.8120.4500.181
- CohortrMeasuresSph.viol0.7830.3810.140
- Object × gen. × coh.rMeasuresSph.viol1.0540.3770.407
- Gen. × coh.rMeasuresSph.viol0.8120.4500.181
GenotypeCohortGenotype × cohortPairwise comparisons
Object explorationTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Object #1, time (%)2wANOVANonnormal39.5 ± 2.1943 ± 1.7140.58 ± 2.741.0290.3650.2191.7450.1930.2540.9630.3890.207
Object #2, time (%)2wANOVANonnormal28.98 ± 1.1627.21 ± 1.227.58 ± 1.390.8050.4530.1801.2310.2730.1932.4000.1020.461
Object #3, time (%)2wANOVANormal17.99 ± 1.1818.73 ± 1.2119.28 ± 1.120.0930.9110.0630.2280.6350.0760.3470.7090.102
Object #4, time (s)2wANOVANormal13.51 ± 0.9211.62 ± 0.9912.54 ± 1.161.0910.3440.2300.4230.5180.0980.7470.4790.169
Repetitive novel object contact task, object preference, number
Object interactions, numberTestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- ObjectrMeasuresSph.viol2.6530.0510.638
- Object × gen.rMeasuresSph.viol0.8580.5280.331
- GenotyperMeasuresSph.viol2.1080.1330.412
- CohortrMeasuresSph.viol73.4750.0001.000
- Object × gen. × coh.rMeasuresSph.viol0.4590.8370.184
- Gen. × coh.rMeasuresSph.viol1.1100.3380.234
GenotypeCohortGenotype × cohortPairwise comparisons
Exploration numbersTestData structureWTHetKOFp valuePowerFpvaluePowerFpvaluePowerWT vs HetWT vs KOHet vsKO
Dice2wANOVANonnormal20.17 ± 1.721.05 ± 1.3920.76 ± 1.690.0540.9480.05815.7070.0000.9730.6200.5420.148
Jack2wANOVANormal20.17 ± 2.4623.42 ± 2.3820.94 ± 1.951.1960.3110.24972.1110.0001.0001.2000.3100.250
Lego2wANOVANormal23.94 ± 2.4626.31 ± 1.7120.82 ± 22.7850.0720.52317.5100.0000.9840.3670.6950.1060.4950.5730.091
Pin2wANOVANormal20.17 ± 2.4623.42 ± 2.3820.94 ± 1.951.1960.3110.24972.1110.0001.0001.2000.3100.250
Object interaction %TestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- ObjectrMeasuresSph.viol4.8120.0220.897
- Object × gen.rMeasuresSph.viol0.3630.7620.151
- GenotyperMeasuresSph.viol0.3280.7220.099
- CohortrMeasuresSph.viol7.3750.0090.758
- Object × gen. × coh.rMeasuresSph.viol0.5600.6270.220
- Gen. × coh.rMeasuresSph.viol0.3280.7220.099
GenotypeCohortGenotype × cohortPairwise comparisons
Object interaction numberTestDatastructureWTHetKOFp valuePowerFpvaluePowerFpvaluePowerWT vs HetWT vs KOHet vsKO
Dice2wANOVANormal24.87 ± 1.4423.13 ± 1.2725.81 ± 1.71.1190.3350.23510.0750.0030.8750.2970.7450.094
Jack2wANOVANormal23.14 ± 1.3524.09 ± 1.0924.69 ± 0.910.1640.8490.07411.9560.0010.9230.5460.5830.135
Lego2wANOVANonnormal28.84 ± 228.66 ± 1.5724.78 ± 1.411.5070.2320.3052.9010.0950.3861.2860.2860.265
Pin2wANOVANonnormal33.55 ± 7.6932.7 ± 6.6430.85 ± 4.830.2350.7920.08510.4760.0020.8870.4670.6300.122
Object interaction number, object ranked by preferenceTestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- ObjectrMeasuresSph.viol74.2240.0001.000
- Object × gen.rMeasuresSph.viol0.8670.4990.335
- GenotyperMeasuresSph.viol2.2280.1190.432
- CohortrMeasuresSph.viol72.2290.0001.000
- Object × gen. × coh.rMeasuresSph.viol0.6530.6490.254
- Gen. × coh.rMeasuresSph.viol1.1420.3280.239
GenotypeCohortGenotype × cohortPairwise comparisons
Object interaction numberTestData structureWTHetKOFp valuePowerFpvaluePowerFp valuePowerWT vs HetWT vs KOHet vsKO
Object #12wANOVANormal26.88 ± 2.329.15 ± 1.8825.58 ± 1.751.6820.1970.33730.5220.0001.0000.1730.8420.075
Object #22wANOVANonnormal23.23 ± 2.0525.15 ± 2.0721.64 ± 1.852.4740.0950.47359.8100.0001.0000.7520.4770.1700.4210.8290.167
Object #32wANOVANonnormal17.94 ± 2.1221.94 ± 1.9218.88 ± 1.72.3070.1100.44666.8440.0001.0001.4600.2420.297
Object #42wANOVANonnormal16.41 ± 1.9117.94 ± 1.3416.94 ± 1.620.4410.6460.11854.9870.0001.0002.3770.1040.457
Object interaction %, object ranked by preferenceTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- ObjectrMeasuresSph.viol86.8850.0001.000
- Object × gen.rMeasuresSph.viol0.7450.5780.288
- GenotyperMeasuresSph.viol1.0100.4110.207
- CohortrMeasuresSph.viol0.9600.3900.163
- Object × gen. × coh.rMeasuresSph.viol0.9790.3270.390
- Gen. × coh.rMeasuresSph.viol0.9600.3900.207
GenotypeCohortGenotype × cohortPairwise comparisons
Object interaction %TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Object #12wANOVANonnormal32.55 ± 1.4131.48 ± 1.0231.19 ± 0.990.0660.9360.05911.3280.0020.9100.9660.3880.208
Object #22wANOVANormal28.09 ± 0.8626.68 ± 0.7425.78 ± 0.81.4330.2490.2920.0090.9260.0510.2560.7750.088
Object #32wANOVANonnormal20.49 ± 0.8322.71 ± 0.8122.52 ± 0.791.5380.2250.3119.1400.0040.8422.0490.1400.402
Object #42wANOVANormal18.85 ± 0.719.11 ± 0.8320.08 ± 0.640.4540.6380.1202.5590.1160.3480.9750.3850.210
Repetitive novel object contact task, pattern of object investigation
GenotypeCohortGenotype × cohortPairwise comparisons
Three-object sequencesTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Total number of 3-object choices2wANOVANormal56.11 ± 3.253.57 ± 4.8253.11 ± 3.290.0770.9260.06112.0530.0010.9250.5730.5670.140
Number of different 3-object sequences2wANOVANormal26.5 ± 0.5725.36 ± 1.2125.47 ± 0.930.4050.6690.1127.5020.0090.7650.8300.4420.184
Number of repetition of top preferred sequence2wANOVANonnormal4.88 ± 0.44.78 ± 0.374.64 ± 0.290.0120.9880.05210.7960.0020.8960.0520.9500.057
Number of repetition of 2nd preferred sequence2wANOVANonnormal4.27 ± 0.274.15 ± 0.334.05 ± 0.260.0170.9830.0528.7480.0050.8260.3120.7330.097
Number of repetition of 3rd preferred sequence2wANOVANonnormal3.83 ± 0.233.68 ± 0.33.7 ± 0.250.0420.9590.0566.5420.0140.7080.3030.7400.095
Number of repetition of top 3 preferred sequences2wANOVANormal13 ± 0.8712.63 ± 0.9812.41 ± 0.780.0080.9920.0519.5450.0030.8570.1460.8650.071
% top preferred sequence choice2wANOVANonnormal8.57 ± 0.329.41 ± 0.618.75 ± 0.191.3240.2760.2720.0000.9940.0501.4360.2480.293
% top 2 preferred sequence choice2wANOVANonnormal16.22 ± 0.5217.48 ± 1.0116.43 ± 0.321.1790.3160.2460.1430.7070.0661.5430.2240.312
% top 3 preferred sequence choice2wANOVANonnormal23.14 ± 0.7124.69 ± 1.4323.44 ± 0.510.8370.4390.1850.5640.4560.1141.0400.3610.221
GenotypeCohortGenotype × cohortPairwise comparisons
Four-object sequencesTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Total number of 4-object choices2wANOVANormal55.55 ± 3.2653.26 ± 4.7952.58 ± 3.240.0670.9350.06010.4000.0020.8850.5280.5930.132
Number of different 4-object sequences2wANOVANormal40.77 ± 1.5939.63 ± 2.7839 ± 2.030.0970.9080.0649.8570.0030.8680.8950.4150.195
Number of repetition of top preferred sequence2wANOVANonnormal3.05 ± 0.23.1 ± 0.23.41 ± 0.171.2970.2830.2674.1440.0470.5140.0500.9510.057
Number of repetition of 2nd preferred sequence2wANOVANonnormal2.83 ± 0.22.84 ± 0.22.76 ± 0.180.0340.9670.0554.3240.0430.5311.2140.3060.253
Number of repetition of 3rd preferred sequence2wANOVANonnormal2.44 ± 0.162.47 ± 0.192.23 ± 0.130.4680.6290.1224.4990.0390.5471.0630.3530.225
Number of repetition of top 3 preferred sequences2wANOVANonnormal8.33 ± 0.538.42 ± 0.558.41 ± 0.420.0870.9160.0635.2670.0260.6140.6340.5350.150
% top preferred sequence choice2wANOVANonnormal5.58 ± 0.36.31 ± 0.476.66 ± 0.272.1870.1230.4252.7590.1030.3701.0560.3560.224
% top 2 preferred sequence choice2wANOVANonnormal10.7 ± 0.4411.99 ± 0.7712.09 ± 0.511.7340.1870.3462.7340.1050.3671.1660.3200.244
% top 3 preferred sequence choice2wANOVANonnormal15.14 ± 0.5416.93 ± 1.0216.43 ± 0.671.5570.2210.3143.1810.0810.4161.2370.2990.257
Barnes maze initial training - distance
DistanceTestData structureFp valuePowerWT vs HetWT vs KOHet vsKO
- DayrMeasuresSph.ass13.6950.0001.000
- Day × gen.rMeasuresSph.ass2.0620.0620.684
- GenotyperMeasuresSph.ass2.6630.0800.5030.6590.1450.515
- CohortrMeasuresSph.ass11.8410.0010.920
- Day × gen. × coh.rMeasuresSph.ass1.1730.3240.416
- Gen. × coh.rMeasuresSph.ass1.1140.3370.234
GenotypeCohortGenotype × cohortPairwise comparisons
Individual daysTestData structureWTHetKOFp valuePowerFp valuePowerFpvaluePowerWT vsHetWT vsKOHet vs KO
Day 12wANOVANormal501.24 ± 48.28485.42 ± 47.71484.49 ± 53.070.0030.9970.0504.2830.0440.5260.0840.9190.062
Day 22wANOVANormal427.6 ± 43.5468.59 ± 40.26504.18 ± 47.171.2340.3010.2569.2050.0040.8441.9180.1580.378
Day 32wANOVANormal292.36 ± 29.11340.26 ± 31.24485.74 ± 41.1611.2930.0000.9896.9020.0120.7303.0820.0550.5670.4960.0000.005
Day 42wANOVANormal311.01 ± 34.75370.86 ± 29.61367.31 ± 42.111.4790.2390.3005.4490.0240.6280.6660.5190.155
Barnes maze reversal - distance
DistanceTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- Day effectrMeasuresSph.ass26.4550.0001.000
- Day × gen.rMeasuresSph.ass2.6120.0230.824
- GenotyperMeasuresSph.ass1.8110.1750.359
- CohortrMeasuresSph.ass1.9240.1720.274
- Day × gen. × coh.rMeasuresSph.ass3.1920.0070.902
- Genotype × cohort effectrMeasuresSph.ass0.2900.7500.093
GenotypeCohortGenotype × cohortPairwise comparisons
Individual daysTestData structureWTHetKOFp valuePowerFpvaluePowerFp valuePowerWT vs HetWT vs KOHet vsKO
Day 12wANOVANonnormal420.93 ± 37.75437.03 ± 37.86591.59 ± 38.485.5920.0070.8340.7930.3780.1411.4750.2390.2990.9480.0090.018
Day 22wANOVANormal336.81 ± 35.36413.64 ± 32.4390.91 ± 451.2850.2860.2650.3740.5440.0922.5250.0910.481
Day 32wANOVANormal357.93 ± 35.96421.04 ± 44.36395.85 ± 48.060.6660.5190.1553.3710.0730.4360.1160.8900.067
Day 42wANOVANormal288.54 ± 39.85288.65 ± 37.41337.24 ± 38.590.9650.3890.2078.8490.0050.8291.3730.2640.281
Barnes maze initial training probe test
GenotypeQuadrant pairwise comparisons
All animalsTestData structureFp valuePowerT vs LT vs RT vs OL vsRL vs OR vs O
- QuadrantrMeasuresSph.viol296.6530.0001.0000.0000.0000.0000.5550.2010.628
- CohortrMeasuresSph.viol10.2000.0021.000
- Quadrant × coh.rMeasuresSph.viol11.4350.0000.983
WTTestData structureFp valuePowerT vs LT vs RT vs OL vs RL vs OR vs O
- QuadrantrMeasuresSph.viol58.3180.0001.0000.0000.0000.0000.0570.1680.335
- CohortrMeasuresSph.viol9.3730.0070.820
- Quadrant × coh.rMeasuresSph.viol4.2410.0100.831
HetTestData structureFp valuePowerT vs LT vs RT vs OL vsRL vsOR vs O
- QuadrantrMeasuresSph.viol107.9800.0001.0000.0000.0000.0000.2050.8950.278
- CohortrMeasuresSph.viol65.3900.0001.000
- Quadrant × coh.rMeasuresSph.viol5.3660.0030.915
KOTestData structureFp valuePowerT vsLT vsRT vsOL vsRL vs OR vs O
- QuadrantrMeasuresSph.viol378.5460.0001.0000.0000.0000.0000.8320.2780.341
- CohortrMeasuresSph.viol890.2260.0001.000
- Quadrant × coh.rMeasuresSph.viol1.6830.1860.406
GenotypeCohortGenotype × cohortPairwise comparisons
Probe test quadrantsTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vsHetWT vsKOHet vsKO
Target2wANOVANonnormal107.29 ± 7.96125.68 ± 7.96142.87 ± 4.535.3420.0080.81612.1440.0010.9270.9350.4000.2020.1120.0010.173
Left2wANOVANonnormal28.57 ± 4.614.87 ± 2.3211.37 ± 2.497.0810.0020.9144.2070.0460.5190.6600.5220.1540.0100.0020.740
Right2wANOVANonnormal16.92 ± 3.8620.57 ± 4.9312.28 ± 2.151.0970.3420.2317.0560.0110.7391.0480.3590.2220.7630.6780.290
Opposite2wANOVANonnormal22.28 ± 4.4914.37 ± 3.479.2 ± 2.092.4380.0990.46610.4430.0020.8861.1490.3260.2400.1990.0240.526
Barnes maze reversal probe test
GenotypeQuadrant pairwise comparisons
All animalsTestData structureFp valuePowerT vs LT vsRT vs OL vsRL vs OR vs O
- QuadrantrMeasuresSph.viol50.8650.0001.0000.0000.0000.0000.2420.0000.000
- CohortrMeasuresSph.viol24.5300.0000.998
- Quadrant × coh.rMeasuresSph.viol4.4430.0050.870
WTTestData structureFp valuePowerT vsLT vsRT vsOL vs RL vs OR vs O
- QuadrantrMeasuresSph.viol32.2790.0001.0000.0000.0000.0000.0050.0240.003
- CohortrMeasuresSph.viol159.3770.0001.000
- Quadrant × coh.rMeasuresSph.viol0.0070.9560.051
HetTestData structureFp valuePowerT vs LT vs RT vs OL vsRL vs OR vs O
- QuadrantrMeasuresSph.viol28.1980.0001.0000.0000.0000.0010.2350.0010.086
- CohortrMeasuresSph.viol6.4120.0210.666
- Quadrant × coh.rMeasuresSph.viol10.3150.0000.998
KOTestData structureFp valuePowerT vsLT vs RT vs OL vs RL vs OR vs O
- QuadrantrMeasuresSph.viol12.0260.0000.9990.0000.0100.6460.0700.0000.000
- CohortrMeasuresSph.viol397.2500.0001.000
- Quadrant × coh.rMeasuresSph.viol2.2730.0950.531
GenotypeCohortGenotype × cohortPairwise comparisons
Probe test quadrantsTestData structureWTHetKOFp valuePowerFp valuePowerFpvaluePowerWT vs HetWT vs KOHet vsKO
Target2wANOVANonnormal115.33 ± 11.27105.35 ± 12.5567.82 ± 11.655.4300.0080.8228.1830.0060.8003.4690.0400.6210.7730.0100.046
Left2wANOVANonnormal18.44 ± 3.768.03 ± 2.159.68 ± 2.793.3430.0440.6042.0790.1560.2920.1720.8420.0750.0390.1230.923
Right2wANOVANonnormal9.8 ± 2.4618.48 ± 7.823.17 ± 5.321.3670.2650.2801.8730.1780.2682.9800.0610.5510.4890.2290.826
Opposite2wANOVANonnormal32.67 ± 6.8742.82 ± 1075.15 ± 10.656.6320.0030.8947.2100.0100.7482.0970.1340.4090.6620.0040.030
  • WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Bold font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 11-1. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, Norm: normal, No-norm: non-normal, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort.