Table 7.

Detailed results and statistical analyses related to the sensory profile

Reflexes and reactions to simple stimuli
GenotypeCohortGenotype × cohortPairwise comparisons
TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Pinna reflex2wANOVANonnormal0.89 ± 0.070.68 ± 0.10.73 ± 0.11.7900.17730.35713.9880.0000.9561.1550.3230.243
Cornel reflex2wANOVANonnormal1.05 ± 0.050.94 ± 0.051.05 ± 0.051.5180.22890.3080.3890.5360.0941.5180.2290.308
Toe pinch retraction2wANOVANonnormal2.05 ± 0.372.36 ± 0.332.26 ± 0.430.1440.86590.0711.0730.3050.1740.0720.9310.060
Preyer reflex2wANOVANonnormal1.47 ± 0.151.36 ± 0.131.42 ± 0.170.1350.87400.07022.2500.0000.9961.4780.2380.301
Visual placingNANA9 ± 09 ± 09 ± 0
Tail flick
LatencyTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol3.0810.05000.583
- Trial × gen.rMeasuresSph.viol0.1690.95350.085
- GenotyperMeasuresSph.viol1.1180.33470.236
- CohortrMeasuresSph.viol83.4670.00001.000
- Trial × gen. × coh.rMeasuresSph.viol0.4890.74380.162
- Gen. × coh.rMeasuresSph.viol2.1620.12550.422
GenotypeCohortGenotype × cohortPairwise comparisons
Individual trialsTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vsKOHet vs KO
Latency trial 1 (s)2wANOVANormal11.39 ± 0.9711.32 ± 0.7810.28 ± 0.820.3560.70230.10413.6800.0010.9520.1450.8650.071
Latency trial 2 (s)2wANOVANormal10.99 ± 1.089.67 ± 0.988.86 ± 0.820.9610.38920.20836.6140.0001.0002.2860.1120.444
Latency trial 3 (s)2wANOVANormal11.05 ± 1.2310.06 ± 1.019.29 ± 0.940.5260.59420.13291.3290.0001.0001.5780.2160.319
Shortest latency (s)2wANOVANormal8.03 ± 0.827.99 ± 0.877.47 ± 0.780.0640.93790.05942.9640.0001.0001.0390.3610.222
Longest latency (s)2wANOVANonnormal14.17 ± 0.9412.82 ± 0.7511.91 ± 0.722.2130.11980.43164.8150.0001.0001.6590.2000.334
Mean latency (s)2wANOVANonnormal11.14 ± 0.8810.35 ± 0.789.48 ± 0.721.1180.33470.23683.4670.0001.0002.1620.1260.422
Startle response
GenotypeCohortGenotype × cohortPairwise comparisons
TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vsHetWT vs KOHet vs KO
Startle 74 dB2wANOVANonnormal179.96 ± 20.24165.57 ± 17.22158.01 ± 10.362.3760.10770.4489.1230.0050.8362.2550.1200.428
Startle 78 dB2wANOVANonnormal183.48 ± 22157.4 ± 16.93160.69 ± 14.893.1810.05380.5715.7790.0220.6473.6730.0360.6380.0460.0250.770
Startle 82 dB2wANOVANonnormal197.45 ± 26.03160.09 ± 14.07175.58 ± 13.873.2540.05000.5823.9390.0550.4882.6210.0870.4870.0190.0630.591
Startle 86 dB2wANOVANonnormal246.33 ± 35.36162.3 ± 15.61176.15 ± 12.883.2550.05000.5820.0820.7770.0590.2710.7640.0890.0240.0420.812
Startle 92 dB2wANOVANonnormal257.25 ± 40.4192.63 ± 19.03201.41 ± 23.012.1530.13130.4110.0390.8450.0541.3230.2790.267
Startle responseTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- Sound intensityrMeasuresSph.ass2.9000.05100.605
- Sound intensity × gen.rMeasuresSph.ass0.6420.66200.219
- GenotyperMeasuresSph.ass3.6490.03640.6350.0220.0240.989
- CohortrMeasuresSph.ass1.8420.18350.262
- Sound intensity × gen. × coh.rMeasuresSph.ass0.8220.53350.276
- Gen. × coh.rMeasuresSph.ass1.9220.16140.372
GenotypeCohortGenotype × cohortPairwise comparisons
TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Startle 74 dB norm. to weight2wANOVANonnormal7.01 ± 0.826.18 ± 0.646.17 ± 0.322.8430.07180.5225.7490.0220.6452.7800.0760.5120.0450.0420.959
Startle 78 dB norm. to weight2wANOVANonnormal7.19 ± 0.895.86 ± 0.646.35 ± 0.583.0510.06010.5532.9720.0940.3893.6110.0380.6300.0380.0360.964
Startle 82 dB norm. to weight2wANOVANonnormal7.74 ± 1.065.89 ± 0.416.98 ± 0.633.3780.04560.5991.7000.2010.2452.3650.1090.4460.0150.0890.409
Startle 86 dB norm. to weight2wANOVANonnormal9.86 ± 1.666 ± 0.497.03 ± 0.642.8880.06900.5290.0300.8640.0530.2680.7660.0890.0280.0700.677
Startle 92 dB norm. to weight2wANOVANonnormal10.04 ± 1.777.09 ± 0.588.18 ± 1.151.7580.18730.3430.3230.5730.0861.2910.2880.261
Startle response normalized to weightTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- Sound intensityrMeasuresSph.ass2.5060.04490.248
- Sound intensity × gen.rMeasuresSph.ass0.5800.79330.098
- GenotyperMeasuresSph.ass3.3380.04710.5930.1270.4070.783
- CohortrMeasuresSph.ass0.4550.50460.101
- Sound intensity × gen. × coh.rMeasuresSph.ass0.7160.59220.079
- Gen. × coh.rMeasuresSph.ass1.8120.17830.353
Pre-pulse inhibition (PPI)
GenotypeCohortGenotype × cohortPairwise comparisons
TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
PPI % (74 dB)2wANOVANormal22.47 ± 5.715.1 ± 4.7210.56 ± 6.030.6250.54090.21211.0610.0020.5191.8210.1770.190
PPI % (78 dB)2wANOVANormal31.81 ± 4.4814.84 ± 6.721.18 ± 5.033.5130.04070.54321.8190.0000.7931.0080.3750.3520.0580.3120.656
PPI % (82 dB)2wANOVANormal30.39 ± 5.5121.53 ± 6.0813.34 ± 5.990.1510.86040.42614.8920.0000.4800.0420.9590.348
PPI % (86 dB)2wANOVANonnormal31.73 ± 7.9521.04 ± 7.3727.04 ± 4.631.4110.25750.17637.1390.0000.9090.4630.6330.217
PPI % (92 dB)2wANOVANonnormal43.62 ± 7.0134.35 ± 5.8229.93 ± 6.970.9890.38210.21254.6830.0000.8922.2580.1200.222
PPI % (average)2wANOVANormal32 ± 5.5321.37 ± 5.4820.41 ± 5.030.1550.85690.30537.4890.0000.8670.3100.7350.278
PPITestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- Sound intensityrMeasuresSph.viol15.3960.00001.000
- Sound intensity × gen.rMeasuresSph.viol1.4090.19430.603
- GenotyperMeasuresSph.viol1.5020.23250.305
- CohortrMeasuresSph.viol9.8060.00290.867
- Sound intensity × gen. × coh.rMeasuresSph.viol0.9780.45400.427
- Gen. × coh.rMeasuresSph.viol1.3490.26860.278
Burried food test
GenotypeCohortGenotype × cohortPairwise comparisons
TestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Latency to retrieve and eat food (s)2wANOVANonnormal51.11 ± 9.994.81 ± 23.69500.28 ± 94.9217.8480.00001.0000.0010.9760.0500.0001.0000.0500.8580.0000.000
Olfactory habituation/dishabituation, sniffing only
WaterTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol8.2900.00190.958
- Trial × gen.rMeasuresSph.viol1.1080.35050.337
- GenotyperMeasuresSph.viol2.9730.06020.5530.6600.0270.066
- CohortrMeasuresSph.viol0.0730.78860.058
- Trial × gen. × coh.rMeasuresSph.viol1.7390.16830.515
- Gen. × coh.rMeasuresSph.viol1.0150.36960.217
BananaTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.ass10.1170.00010.983
- Trial × gen.rMeasuresSph.ass3.9080.00540.888
- GenotyperMeasuresSph.ass5.6810.00600.8420.4330.0020.017
- CohortrMeasuresSph.ass11.9330.00110.923
- Trial × gen. × coh.rMeasuresSph.ass0.4860.74610.161
- Gen. × coh.rMeasuresSph.ass0.1340.87520.069
LemonTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol6.6990.00410.908
- Trial × gen.rMeasuresSph.viol0.0470.98900.059
- GenotyperMeasuresSph.viol2.7150.07600.5130.4040.1660.025
- CohortrMeasuresSph.viol15.3270.00030.970
- Trial × gen. × coh.rMeasuresSph.viol0.6670.58280.211
- Gen. × coh.rMeasuresSph.viol0.1590.85340.073
Male scentTestData structureFpvaluePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol27.9030.00001.000
- Trial × gen.rMeasuresSph.viol1.0890.35810.332
- GenotyperMeasuresSph.viol0.7390.48280.168
- CohortrMeasuresSph.viol2.5000.12010.341
- Trial × gen. × coh.rMeasuresSph.viol1.6030.19760.479
- Gen. × coh.rMeasuresSph.viol0.5410.58570.134
Female scentTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol20.9220.00001.000
- Trial × gen.rMeasuresSph.viol0.1310.93210.076
- GenotyperMeasuresSph.viol0.5850.56090.142
- CohortrMeasuresSph.viol14.7710.00030.965
- Trial × gen. × coh.rMeasuresSph.viol0.3480.77650.126
- Gen. × coh.rMeasuresSph.viol1.6510.20220.332
GenotypeCohortGenotype × cohortPairwisecomparisons
Individual trialsTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Water 12wANOVANonnormal1.82 ± 0.371.81 ± 0.420.9 ± 0.162.2050.12080.4290.1990.6580.0721.6970.1940.340
Water 22wANOVANonnormal1.02 ± 0.150.98 ± 0.140.7 ± 0.151.4380.24700.2940.0780.7810.0590.7110.4960.163
Water 32wANOVANonnormal0.97 ± 0.150.82 ± 0.140.7 ± 0.111.7240.18880.3452.9000.0950.3860.3660.6960.106
Banana 12wANOVANonnormal1.34 ± 0.121.04 ± 0.230.44 ± 0.158.7420.00060.9616.2010.0160.6850.4660.6300.1220.3220.0010.052
Banana 22wANOVANonnormal0.65 ± 0.120.65 ± 0.10.44 ± 0.131.6410.20400.3306.6580.0130.7160.2400.7870.086
Banana 32wANOVANonnormal0.52 ± 0.090.72 ± 0.190.44 ± 0.131.1670.31960.2457.7610.0080.7800.0500.9510.057
Lemon 12wANOVANonnormal0.58 ± 0.180.77 ± 0.150.52 ± 0.170.7200.49160.1658.5720.0050.8190.0410.9600.056
Lemon 22wANOVANonnormal0.39 ± 0.110.52 ± 0.10.24 ± 0.072.6790.07850.5079.7840.0030.8660.0370.9640.0550.7090.4110.099
Lemon 32wANOVANonnormal0.35 ± 0.060.48 ± 0.090.25 ± 0.082.5470.08850.4867.4050.0090.7612.4930.0930.4780.5940.5400.106
Male 12wANOVANonnormal6.1 ± 1.124.66 ± 0.884.94 ± 1.070.6590.52190.1541.0950.3000.1771.0910.3440.231
Male 22wANOVANonnormal2.25 ± 0.342.77 ± 0.461.26 ± 0.314.0950.02250.7000.5640.4560.1143.0780.0550.5680.7520.1160.020
Male 32wANOVANonnormal1.54 ± 0.381.25 ± 0.241.72 ± 0.630.3220.72640.0982.4650.1230.3370.3120.7330.097
Female 12wANOVANonnormal5.68 ± 0.986.43 ± 1.346.32 ± 1.930.1290.87900.06911.5090.0010.9140.8790.4220.193
Female 22wANOVANonnormal2.18 ± 0.32.24 ± 0.432.62 ± 0.810.1890.82830.0781.9360.1700.2760.2890.7510.093
Female 32wANOVANonnormal1.42 ± 0.261.82 ± 0.522.76 ± 0.881.6000.21200.3237.6720.0080.7751.7920.1770.357
Olfactory habituation/dishabituation, all interactions
WaterTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol1.8910.16640.385
- Trial × gen.rMeasuresSph.viol0.7180.54780.225
- GenotyperMeasuresSph.viol7.2100.00170.9200.6370.0010.015
- CohortrMeasuresSph.viol2.6390.11040.357
- Trial × gen. × coh.rMeasuresSph.viol0.9200.43710.283
- Gen. × coh.rMeasuresSph.viol1.1330.33000.239
BananaTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol5.2290.01330.821
- Trial × gen.rMeasuresSph.viol1.2820.28660.388
- GenotyperMeasuresSph.viol5.7370.00570.8460.7440.0600.008
- CohortrMeasuresSph.viol7.9220.00700.788
- Trial × gen. × coh.rMeasuresSph.viol0.9360.42840.287
- Gen. × coh.rMeasuresSph.viol0.9900.37870.213
LemonTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol1.3030.27280.276
- Trial × gen.rMeasuresSph.viol0.7030.55970.221
- GenotyperMeasuresSph.viol4.8930.01150.7810.2950.0480.003
- CohortrMeasuresSph.viol5.1520.02760.605
- Trial × gen. × coh.rMeasuresSph.viol0.5270.67380.172
- Gen. × coh.rMeasuresSph.viol2.4050.10060.463
Male scentTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.viol28.6520.00001.000
- Trial × gen.rMeasuresSph.viol0.7900.49930.246
- GenotyperMeasuresSph.viol5.7220.00570.8450.8390.0050.022
- CohortrMeasuresSph.viol2.9530.09180.392
- Trial × gen. × coh.rMeasuresSph.viol0.3670.76970.131
- Gen. × coh.rMeasuresSph.viol0.0090.99060.051
Female scentTestData structureFp valuePowerWT vs HetWT vs KOHet vs KO
- TrialrMeasuresSph.ass25.0440.00001.000
- Trial × gen.rMeasuresSph.ass0.8840.47620.264
- GenotyperMeasuresSph.ass1.1190.33460.236
- CohortrMeasuresSph.ass6.4000.01450.699
- Trial × gen. × coh.rMeasuresSph.ass1.9110.11430.541
- Gen. × coh.rMeasuresSph.ass0.4310.65210.116
GenotypeCohortGenotype × cohortPairwise comparisons
Individual trialsTestData structureWTHetKOFp valuePowerFp valuePowerFp valuePowerWT vs HetWT vs KOHet vs KO
Water 12wANOVANonnormal3.52 ± 0.93.88 ± 11.35 ± 0.272.3570.10530.4553.3020.0750.4291.1160.3360.235
Water 22wANOVANonnormal3.45 ± 0.711.78 ± 0.480.95 ± 0.234.9640.01090.7860.5880.4470.1171.2950.2830.2670.1610.0060.364
Water 32wANOVANonnormal2.53 ± 0.542.3 ± 0.60.95 ± 0.22.9970.05920.5560.0330.8560.0540.3730.6900.1070.9490.0780.147
Banana 12wANOVANonnormal2.8 ± 0.423.38 ± 1.160.72 ± 0.433.8540.02790.6726.6030.0130.7121.0800.3480.229
Banana 22wANOVANonnormal1.46 ± 0.491.06 ± 0.350.57 ± 0.181.9020.16010.3764.9910.0300.5911.0560.3550.224
Banana 32wANOVANonnormal1.38 ± 0.552 ± 0.630.4 ± 0.13.9270.02620.6801.9200.1720.2741.0970.3420.2320.3340.3800.020
Lemon 12wANOVANonnormal1.47 ± 0.372.21 ± 0.740.45 ± 0.193.7070.03170.6532.7100.1060.3650.8780.4220.1930.4120.3390.024
Lemon 22wANOVANonnormal1.21 ± 0.350.98 ± 0.360.28 ± 0.092.5360.08950.4843.6090.0630.4611.0120.3710.2160.7810.0910.315
Lemon 32wANOVANonnormal0.8 ± 0.180.61 ± 0.150.2 ± 0.081.3620.26560.2801.6150.2100.2381.3340.2730.275
Male 12wANOVANonnormal55.55 ± 6.7348.98 ± 7.2227.15 ± 6.113.3410.04360.6052.4190.1260.3320.0540.9480.0580.6550.0300.199
Male 22wANOVANonnormal27.09 ± 6.0129.19 ± 5.337.48 ± 4.344.7530.01300.7681.1960.2800.1890.0120.9880.0520.9860.0200.030
Male 32wANOVANonnormal17.57 ± 5.514.39 ± 4.435.26 ± 1.632.3230.10870.4491.3290.2550.2040.9780.3830.210
Female 12wANOVANonnormal53.65 ± 5.9750.59 ± 5.4831.16 ± 7.211.7810.17920.3556.1180.0170.6791.5650.2190.316
Female 22wANOVANonnormal23.26 ± 6.3718.85 ± 6.7714.61 ± 4.30.4270.65510.1152.1160.1520.2970.3500.7070.103
Female 32wANOVANonnormal14.57 ± 3.8511.5 ± 3.6914.21 ± 4.610.2690.76500.0900.5750.4520.1151.7030.1930.341
  • WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Bold font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 7-1. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort.