Table 10.

Detailed results and statistical analyses related to the hyper-reactivity and escape behavior

Reflexes and reactions to simple stimuli
						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
	Test	Data structure	WT	Het	KO	F	p value	Power	F	p value	Power	F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Touch escape	2wANOVA	Nonnormal	1.26 ± 0.1	1.15 ± 0.11	2 ± 0.15	12.962	0.000	0.996	0.046	0.831	0.055	0.862	0.428	0.190	0.648	0.000	0.000
Positional passivity (sum)	2wANOVA	Nonnormal	2.15 ± 0.25	1.84 ± 0.23	2.84 ± 0.2	11.737	0.000	0.992	106.722	0.000	1.000	1.993	0.147	0.393	0.034	0.011	0.000
Positional passivity (score)	2wANOVA	Nonnormal	1.78 ± 0.24	2.21 ± 0.21	0.94 ± 0.2	14.029	0.000	0.998	44.935	0.000	1.000	3.871	0.027	0.675	0.034	0.004	0.000
Catalepsy (4 trials)	2wANOVA	Nonnormal	2.98 ± 0.57	2.75 ± 0.54	0.56 ± 0.25	7.578	0.001	0.933	4.681	0.035	0.565	1.116	0.336	0.236	0.836	0.001	0.002
Trunk curl	2wANOVA	Nonnormal	1 ± 0	0.89 ± 0.07	1 ± 0	2.547	0.088	0.487	2.537	0.117	0.346	2.547	0.088	0.487	0.057	1.000	0.056
Negative geotaxis, latency to turn	2wANOVA	Nonnormal	6.73 ± 1.06	8.21 ± 1.9	3.22 ± 0.58	4.201	0.020	0.713	2.978	0.090	0.395	0.707	0.498	0.163	0.499	0.042	0.008

Beam walking
						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
	Test	Data structure	WT	Het	KO	F	p value	Power	F	p value	Power	F	p value	Power	WT vsHet	WT vsKO	Het vs KO
Latency to cross (large, s)	2wANOVA	Nonnormal	8.34 ± 2.79	10.8 ± 4.52	3.96 ± 1.75	1.163	0.321	0.244	0.251	0.618	0.078	1.300	0.281	0.269	−	−	−
Latency to cross (medium, s)	2wANOVA	Nonnormal	15.9 ± 4.8	7.17 ± 4.16	7.65 ± 6.13	0.811	0.450	0.181	3.349	0.073	0.435	0.121	0.887	0.068	−	−	−
Latency to cross (small, s)	2wANOVA	Nonnormal	54.71 ± 5.89	46.15 ± 7.59	33.72 ± 7.84	2.204	0.121	0.430	0.235	0.630	0.076	0.437	0.648	0.117	−	−	−

Escape behavior
						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
	Test	Datastructure	WT	Het	KO	F	p value	Power	F	p value	Power	F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Buried food, number of attempts	2wANOVA	Nonnormal	0.63 ± 0.35	0.68 ± 0.32	0.42 ± 0.31	0.666	0.519	0.155	1.738	0.194	0.253	0.220	0.804	0.082
Buried food, % of escapers	2wANOVA	Nonnormal	21.05 ± 9.6	26.31 ± 10.37	15.78 ± 8.59	0.760	0.473	0.172	3.639	0.062	0.464	0.159	0.853	0.073
Four-object, number of attempts	2wANOVA	Nonnormal	0.41 ± 0.21	2.05 ± 0.73	3.88 ± 1.21	5.323	0.008	0.815	5.320	0.025	0.618	3.316	0.045	0.601	0.187	0.002	0.050
Four-object, % of escapers	2wANOVA	Nonnormal	23.52 ± 10.6	36.84 ± 11.36	50 ± 12.12	1.502	0.233	0.305	4.351	0.042	0.534	2.575	0.087	0.490
Marble burying, number of attempts	2wANOVA	Nonnormal	4.32 ± 1.28	10.63 ± 1.98	16.05 ± 2.38	8.063	0.001	0.946	6.649	0.013	0.715	1.239	0.299	0.257	0.034	0.000	0.055
Marble burying, % of escapers	2wANOVA	Nonnormal	47.36 ± 11.76	94.73 ± 5.26	100 ± 0	12.009	0.000	0.993	7.713	0.008	0.777	4.474	0.017	0.740	0.000	0.000	0.598

WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Red font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 10-1. 2wANOVA: 2-way ANOVA.