Outcomes of video screening of acute seizures and hippocampal histology to verify KA injection targeting
| Video screening outcome (acute seizures) | ||||
|---|---|---|---|---|
| >2 seizures | 1 seizure | No seizures or video | Total | |
| Females | 57 | 5 | 7 | 69 |
| Males | 10 | 0 | 5 | 15 |
| Histology for mice with no seizures or video (2 months post-KA injection) | ||||
| Sclerosis | Gliosis | No sclerosis or gliosis | Total | |
| Females | 4 | 2 | 1* | 7 |
| Males | 5 | 0 | 0 | 5 |
Number of seizures refers to behavioral seizures (Racine stage 3 or higher) observed within 5 h of KA injection. Hippocampal tissue from mice that either did not show acute seizures or for whom videos were not available was collected approximately two months after KA injection. Sclerosis was assessed via cresyl violet (Nissl) staining. Sections of hippocampi that did not display signs of sclerosis were further assessed for gliosis via GFAP staining; *, mouse removed from dataset in absence of either video confirmation of acute seizure induction or later development of hippocampal sclerosis/gliosis.