Nucleotide sequences of primers used in this study
| Forward primer | Reverse primer | |
|---|---|---|
| Degenerate primers | ||
| egr | 5′-GGA GTN CAR CTN GCH GAR TA-3′ | 5′-GAN CGC ATG CAD ATN CGR CAY TG-3′ |
| 5′-ACS AGN AAR GGN CAY GAR AT-3′ | 5′-TTN AGR TGN ACY TTN GCR TG-3′ | |
| 5′-CCA RTG YCG NAT HTG CAT GCG-3′ | ||
| hr38 | 5′-AAC CGC TGC CAR TTY TGC-3′ | 5′-AGA AGC TCC TGR TCR TNG C-3′ |
| tdc2 | 5′-TCG AGT ACG CSG AYT CKT TCA ACA C-3′ | 5′-GGA TCR CTS ACC ATN CGN ACG AAG AA-3′ |
| Primers for full-length ORF amplification | ||
| fra-A | 5′-CGC GGG AGT AAG GAC GTG-3′ | 5′-CCC CAT TGT CCA AAT CCT CC-3′ |
| fra-B | 5′-GGC GGC TTG TGT GTT TGT G-3′ | 5′-CCC CAT TGT CCA AAT CCT CC-3′ |
| jra | 5′-GAC GGT CGC GGA GAG TC-3′ | 5′-GAT CTC ATA TGT ATA TGC ATG TGT TCA C-3′ |
| egr-B | 5′-TTC ATT CAT AAA AGT GTT GTA GAG CG-3′ | 5′-ATA TAT ACG AAT CGA GGA GAA CAC-3′ |
| tdc1 | 5′-CAT CTG GCG TTC GCT C-3′ | 5′-CGC AGT CCC AGA AGA G-3′ |
| tdc2 | 5′-CGA CGC CCG ACG ACA TTC G-3′ | 5′-CCG GCT CGT ATG TTG TGT GG-3′ |
| Primers for RT-qPCR | ||
| fra total transcripts | 5′-GGA CGG CCT CAA TTC GGG-3′ | 5′-GGA TTC CAC CTC GCA CTG C-3′ |
| fra-A | 5′-CCT GCC TTC ATC TGC GTA CG-3′ | 5′-GTC TCA CTG GGC GAA ACG TG-3′ |
| fra-B | 5′-GGC GGC TTG TGT GTT TGT G-3′ | 5′-GGA TTC CAC CTC GCA CTG C-3′ |
| jra | 5′-GAG CGG ACG GTT GTG TTA GG-3′ | 5′-GCA GTT GCG TAC CAT CTA AAT CC-3′ |
| egr-B (for initial expression analysis) | 5′-GAC CTA GGC GTC GAA CCC-3′ | 5′-GTT CCA AGG ATC CTG TGA TGG G-3′ |
| egr-B | 5′-GTT TGG AAA CGC TGA GCC C-3′ | 5′-CCT GAC GCT GTA GAG GCA C-3′ |
| egr-B pre-mRNA | 5′-GTG ACA CAT GTA ATT GGC GTA AC-3′ | 5′-CAA TTC CTC GGG TTC CAA GG-3′ |
| hr38 | 5′-CCA ACC TCG ACT ATT CAC AGT ATC-3′ | 5′-CCG GAA TCT TAT CAG CAA ACG TG-3′ |
| hr38 pre-mRNA | 5′-GAA GCA TCT ACT CCA GTC TCA TAA TAG-3′ | 5′-GTA GGC TCA CGA TAC TGG AAA TG-3′ |
| EYFPnls:PEST | 5′-CGA GGA GCT GTT CAC CGG-3′ | 5′-GGT GCA GAT GAA CTT CAG GG-3′ |
| β-actin | 5′-CGT AAA CTC AAC TAC TAA CCA TGT GC-3′ | 5′-GCC CTG GGT GCA TCA TCG-3′ |
| ef1α | 5′-CGA CTC CGG TAA ATC TAC GAC C-3′ | 5′-CAC CCA GGC ATA CTT GAA AGA AC-3′ |
| rpl32 | 5′-CGC CCA GTT TAT CGT CCA AC-3′ | 5′-GCC TGC GAA CTC TGT TGT C-3′ |
| Primers used to amplify the core promoter regions of orthopteran egr-B homologs | ||
| 5′-GTT ACG TCA TTT GAC GTC A-3′ | 5′-GTC CCA TAT TTG GAA GTC G-3′ | |
| 5′-GTT ACG TCA TTT TGA CGT CA-3′ | 5′-CAM CAS TTT TAT GAA TGA AG-3′ | |
| Primers used to amplify the genomic DNA fragment upstream to the coding sequence of the orthopteran egr-B homologs | ||
| Gryllus bimaculatus | 5′-CAG GGG TTG TTT ATT CGC CG-3′ | 5′-CTG TGA TGG GAG GCG GTT CAA C-3′ |
| Acheta domesticus | 5′-AAA TTC GAA AGC CTT GAC AGT GG-3′ | 5′-ACG ATG GAC GAG CGT CGT G-3′ |
| Gampsocleis buergeri | 5′-ATG TTC CCC CTC CAT GCC AG-3′ | 5′-ACA TGC TGA CGC GCA ACA C-3′ |
| Locusta migratoria | 5′-CAG TGT TGC CAG CCT CC-3′ | 5′-CCG ACG AGT ACA GGC AGT C-3′ |