Table 3.

Quantification of GFP staining intensity as a proxy for calcium signalling and neuronal activity levels

GenotypeTH GAL4 > UAS CaLexAPdfr5304; TH GAL4 > UAS CaLexA
Time pointZT4ZT14ZT4ZT14
n (hemispheres)14161312
PAL30.4 ± 4.832.3 ± 5.750.3 ± 5.936.8 ± 6
PAM27.3 ± 13.855.2 ± 19.236.7 ± 10.60
PPM16.4 ± 0.3000
PPM223.4 ± 5.241.3 ± 5.936.9 ± 6.723.8 ± 4.3
PPM317.2 ± 372.5 ± 12.175.8 ± 8.769.6 ± 7.6
PPL122 ± 9.917.4 ± 3.559.2 ± 41.322.6 ± 4.6
PPL2ab15.420.2 ± 2.849.5 ± 10.936.7 ± 5.9
PPL2c0018.733.6
PPL36 ± 1.523.6 ± 8.611.4 ± 1.952.2 ± 0.6
PPL4015.800
  • GFP+ fluorescence intensity (mean ± SEM) in different subsets of dopaminergic neurons.